2-dimensional gel electrophoresis
Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis
... 2-DE: 2-dimensional electrophoresis; Apo A1: Apolipoprotein A1; ATS: American Thoracic Society; BAL: bronchoalveolar lavage; BCA: bicinchoninic acid; BO: bronchiolitis obliterans; CBB: ...
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Proteomic studies of pseudomonas putida KT2440 in carcinogenicity screening via 2-dimensional gel electrophoresis
... apply 2-DE for the comparison of protein patterns expressed by ...one gel was clearer than that of another ...convert gel images to digital file are of ...
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Proteome analysis of vaccinia virus IHD W infected HEK 293 cells with 2 dimensional gel electrophoresis and MALDI PSD TOF MS of on solid phase support N terminally sulfonated peptides
... Despite remarkable progress in the control and treat- ment of infectious diseases, the problem of emerging and re-emerging pathogens is likely to be one of the main issues of medical science and public health in the ...
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The impact of growth hormone on proteomic profiles: a review of mouse and adult human studies
... difference gel electrophoresis; 2DE: 2‑dimensional gel electrophoresis; 2DE + MS/MS: 2‑dimensional gel electro‑ phoresis plus tandem mass spectrometry; A2M: ...
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Optimizing Human Synovial Fluid Preparation for Two-Dimensional Gel Electrophoresis
... II electrophoresis equipment and Tris-glycine buffer (25 mM Tris, 192 mM glycine) containing ...per gel and with the temperature at ...Protein Gel Stain (Molecular Probes, ...SF 2-DE ...
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Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach
... Xenopus egg extract preparation, chromatin reconstitution, and elution of chromosomal proteins was carried out as previously described [27,28,20]. For IEF, methanol/chlo- roform precipitated samples containing 50 µg ...
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Antiproliferative heparan sulfate inhibiting hyaluronan and transforming growth factor-β expression in human lung fibroblast cells
... two-dimensional gel electrophoresis (2-DE) on heparan sulfate (HS)-stimulated human fetal lung (HFL)-1 ...urea, 2 M thiourea, 2% CHAPS). The lysed cells were separated by ...
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Biomarkers of HIV-1 associated dementia: proteomic investigation of sera
... used 2-dimensional electro- phoresis (2DE) with Differential Gel Electrophoresis (DIGE) profiling method of immunodepleted CSF or sera from HIV-1 infected individuals with or without HAD ...
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Preterm intraamniotic infection and inflammation: Search for protein biomarkers via proteomics approach
... two-dimensional gel electrophoresis (2-DE) method separates proteins using two different physiochemical ...polyacrylamide gel electrophoresis (SDS_PAGE) [130]. Finally, the ...
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Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis
... to 2-DE Protein sample prefractionation before 2-DE has been implemented to reduce sample ...the 2-DE gel and leads to better resolution, visualization and ...
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Identification of Immunologically Relevant Proteins of Chlamydophila abortus Using Sera from Experimentally Infected Pregnant Ewes
... with 2 ⴛ 10 6 inclusion forming units (IFU) of ...and 2-D gel electrophoresis; reactive antigens identified by mass spectrometry included the major outer membrane protein (MOMP), polymor- phic ...
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Estimation of mutation rates based on the analysis of polypeptide constituents of cultured human lymphoblastoid cells.
... In the present study, we estimate human somatic mutation rates at multiple, unselected loci by applying two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) for ma[r] ...
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Two–Dimensional Gel Electrophoresis of Ceolomic Fluid of Eisenia foetida Earthworm
... (8), anti-oxidant (9), and antibacterial (10) properties. The function of coelomocytes, cells that are presented within the coelomic fluid, remains unclear. A detailed knowledge about their protein contents is required ...
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Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis
... 2. Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Col- lins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang ...
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Clustering of Clinical Strains ofHelicobacter pylori Analyzed by Two-Dimensional Gel Electrophoresis
... for 2-D PAGE and immu- noblotting were obtained from Amersham Pharmacia Biotech, Uppsala, ...II electrophoresis system according to the manufacturers’ ...
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Increase in local protein concentration by field-inversion gel electrophoresis
... Mouse liver protein lysate was prepared as described pre- viously [28]. Protein concentrations were measured using the MicroBCA method (Pierce, Rockford, IL) with bovine serum albumin as a standard. Typically 100 μg of ...
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The identification of human aldo-keto reductase AKR7A2 as a novel cytoglobin-binding partner
... Whole cell extracts of transfected LX-2 cells were prepared 48 h post-transient trans- fection in a mild lysis buffer consisting of 20 mM Tris, 150 mM NaCl, 2 % glycerol, 1.6 mM EDTA, 0.5 % Triton X-100 and ...
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Comparison of staining methods for two dimensional electrophoresis gel resolved with Puntius javanicus liver proteome
... slab gel and electrophoresis was performed at 16mA for 30 minutes and 24 mA for 2 hours and 30 ...the electrophoresis was completed, the gel was washed with water for 10 minutes and ...
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Comparison of Proteome Profiling of Two Sensitive and Resistant Field Iranian Isolates of Leishmania major to Glucantime® by 2- Dimensional Electrophoresis.
... Bio- Rad GS- 800 densitometer and analyzed by Image Master 2D Melanie- 6 software. Each set of gel replicates for both the sensi- tive and resistant L. major isolates was com- bined into the average gels, which ...
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Genetic definition of a new bovine papillomavirus type 1 open reading frame, E5B, that encodes a hydrophobic protein involved in altering host-cell protein processing.
... We have previously utilized ultra-high-resolution "giant" two-dimensional 2-D gel electrophoresis of metabolically labeled proteins to identify the specific changes in host cell protein [r] ...
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