23S rRNA
Development of a reference data set for assigning Streptococcus and Enterococcus species based on next generation sequencing of the 16S–23S rRNA region
... 16S rRNA (1284- bp), sodA (430-bp), tuf (830-bp) and rpoB (740-bp) gene frag- ments was considered as unambiguous for sequences differ- ent in at least 3, 2, 3 and 3 nucleotides, ...16S–23S rRNA ...
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Use of 16S rRNA, 23S rRNA, and gyrB Gene Sequence Analysis To Determine Phylogenetic Relationships of Bacillus cereus Group Microorganisms
... 16S rRNA and partial (more then 60% of the whole gene length) gyrB sequences for ...16S rRNA sequences (Fig. 1 and Table 2). Additional information about the 23S rRNA obtained from ...
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16 23S rRNA Spacer Region Polymorphism in Gangetic River Water Isolates of Salmonella
... 16-23S rRNA spacer re- gion has been reported to be polymorphic at serovar level in ...16-23S rRNA spacer region ...16-23S rRNA spacer region is not spe- cific at serovar level, ...
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Analysis of the 16S–23S rRNA Gene Internal Transcribed Spacer Region in Klebsiella Species
... Amplification of ITS regions. Primers wl-5793 (5⬘-TGT ACA CAC CGC CCG TC-3 ⬘ ) and wl-5794 (5 ⬘ -GGT ACT TAG ATG TTT CAG TTC-3 ⬘ ), designed based on the most conserved sequences at the end of the 16S rRNA gene ...
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Identification of novel insertion elements, restriction fragment length polymorphism patterns, and discontinuous 23S rRNA in Lyme disease spirochetes: phylogenetic analyses of rRNA genes and their intergenic spacers in Borrelia japonica sp nov and genomic group 21038 (Borrelia andersonii sp nov ) isolates
... the 23S rRNA gene copies are fragmented, in 19857 and 21038 only the rrlB genes are ...the 23S rRNA ...the 23S rRNA molecule, while the 1,600-base fragment carries the 3 9 ...
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Identification of Acinetobacter isolates in the A calcoaceticus A baumannii complex by restriction analysis of the 16S 23S rRNA intergenic spacer sequences
... one of the genospecies (A. calcoaceticus) to be recognized on this basis. However, because the similarities of the amplified DNA fragments hindered their electrophoretic separation, a restriction analysis was performed ...
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Evaluation of a fluorescence labelled oligonucleotide probe targeting 23S rRNA for in situ detection of Salmonella serovars in paraffin embedded tissue sections and their rapid identification in bacterial smears
... A method for the detection of Salmonella based on fluorescence in situ hybridization (FISH) has been developed and applied for the direct detection of Salmonella in pure cultures and in formalin-fixed, paraffin- embedded ...
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Potential Limitations of the 16S 23S rRNA Intergenic Region for Molecular Detection of Bartonella Species
... plants, and water (11, 32). Therefore, the potential detection of Mesorhizobium DNA in non-molecular-grade water is not a surprising finding. As illustrated in this study, contamination of some sources of molecular-grade ...
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Mutation at the position 2058 of the 23S rRNA as a cause of macrolide resistance in Streptococcus pyogenes
... The strain ni4277 had elevated MICs for ketolides, when compared to the control strain ATCC 700294. Telithro- mycin MIC 16 µg/ml was so high, that although there are no breakpoints available for telithromycin, this ...
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Molecular Epidemiologic Analysis and Antimicrobial Resistance of Helicobacter cinaedi Isolated from Seven Hospitals in Japan
... cinaedi 23S rRNA sequence, corresponds to mutations at posi- tions 2143 in ...the 23S rRNA gene of erythromycin-susceptible and erythromycin-resistant ...
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Novel base triples in RNA structures revealed by graph theoretical searching methods
... four 23S rRNA struc- tures used showed that several triple locations have vari- able base content, although the equivalent base positions superpose well and occupy a similar structural space (Figure ...
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Mycoplasma genitalium Macrolide and Fluoroquinolone Resistance Detection and Clinical Implications in a Selected Cohort in New Zealand
... In total, 157 M. genitalium DNA-positive samples were collected. Specimen sites included 114 (72.6%) urine samples and 43 (27.4%) swabs (vaginal, cervical, and rectal). Only 115 (73.3%) samples from 104 patients produced ...
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A 5′ Nuclease Genotyping Assay for Identification of Macrolide Resistant Mycoplasma genitalium in Clinical Specimens
... Sanger sequencing of 23S rRNA. Using Sanger sequencing, we were able to amplify and sequence the 23S rRNA gene in 253/259 (97.7%; 95% confidence interval [CI], 95.9% to 99.5%) samples. Of the ...
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Antibiotic Resistance of Helicobacter pylori Strains in Japanese Children
... Detection of gene mutations. On the basis of an analysis with BsaI digestion, the A2144G mutation in the 23S rRNA gene were detected in 11 of 12 (92%) clarithromycin-resistant strains tested (Fig. 2). In ...
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<p>Phenotype and Molecular Detection of Clarithromycin and Levofloxacin Resistance in <em>Helicobacter pylori</em> Clinical Isolates in Beijing</p>
... Results: From the 176 clinical samples, 112 (112/176, 63.6%) were con fi rmed with H. pylori infection and 65 (65/176, 36.9%) active H. pylori strains were obtained and further con fi rmed by MIC assay. Overall, the rates ...
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Genotypic Identification of Erythromycin Resistant Campylobacter Isolates as Helicobacter Species and Analysis of Resistance Mechanism
... 16S rRNA genes of 35 ...the 23S rRNA gene of ...the 23S rRNA at the erythromycin-binding site (28, ...the 23S rRNA genes from 22 erythromycin-resistant Campy- lobacter ...
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Complete genome sequence of the extremely acidophilic methanotroph isolate V4, Methylacidiphilum infernorum, a representative of the bacterial phylum Verrucomicrobia
... of 23S rRNA sequences [17]. Recent phylogenetic analyses of 16S rRNA and ribosomal protein sequences have led to the proposal that four bac- terial phyla; namely Planctomycetes, Verrucomicrobia, ...
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Direct and Rapid Detection by PCR ofErysipelothrix sp DNAs Prepared from Bacterial Strains and Animal Tissues
... 16S rRNA sequences of all four Erysipelothrix ...the rRNA gene cluster, including 16S rRNA, 23S rRNA, and the noncoding region downstream of 5S rRNA, were determined in order to ...
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Non-contiguous finished genome sequence and description of Paenibacillus senegalensis sp. nov.
... is 5,581,254 bp long (1 chromosome but no plas- mid) with a 48.2% G + C content of (Figure 5 and Table 3). Of the 5,059 predicted genes, 5,008 were protein-coding genes, and 51 were RNAs. Nine rRNA genes (three ...
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Species Identification and Subtyping ofUreaplasma parvum and Ureaplasma urealyticumUsing PCR Based Assays
... 16S rRNA gene and 16S rRNA-23S rRNA intergenic spacer regions, the urease gene subunits, and the 5 ⴕ ends of the multiple-banded antigen (MBA) genes, to identify and subtype these Ureaplasma ...
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