28S RDNA
The ribosomal transcription units of Haplorchis pumilio and H taichui and the use of 28S rDNA sequences for phylogenetic identification of common heterophyids in Vietnam
... Partial 28S rDNA sequences were obtained from 14 samples of Vietnamese heterophyids representing six species: Centrocestus formosanus, Haplorchis pumilio, ...
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Phylogenetic Location of the Spirometra Sparganum Isolates from China, Based on Sequences of 28S rDNA D1.
... aligned 28S rDNA D1 sequences were analyzed by using three methods of Neighbor- Joining (NJ), maximum parsimony (MP) and Bayesian inference (BI), ...
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Targeted gene knockin in zebrafish using the 28S rDNA-specific non-LTR-retrotransposon R2Ol
... To create R2Ol in vitro transcription template vector pR2Olr4, a full-length R2Ol fragment from pGEM-R2Ol-F digested with EcoRI and SpeI, was inserted into EcoRI and SpeI sites and an SV40 poly(A) signal was inserted ...
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The ribosomal transcription units of Haplorchis pumilio and H. taichui and the use of 28S rDNA sequences for phylogenetic identification of common heterophyids in Vietnam
... Partial 28S rDNA sequences were obtained from 14 samples of Vietnamese heterophyids representing six species: Centrocestus formosanus, Haplorchis pumilio, ...
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Molecular Analysis, Based on 28s rDNA Dctylogyroides Species, Parasitizing Puntius Species
... Traditional study was based, to a large extent, on the morphology of the sclerotized components of the haptoral parts. In recent times, molecular techniques are being increasingly used in taxonomy and phylogenetic ...
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Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... gion. For example, many closely related species of Aspergillus and Penicillium are not able to be discriminated by the 28S rDNA sequence, and sequencing analysis initially ascertains only the genus of the ...
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Genetic characterization of far eastern species of the genus Crepidostomum (Trematoda: Allocreadiidae) by means of 28S ribosomal DNA sequences
... The 28S rRNA gene was amplified using the polyme- rase chain reaction (PCR) with the following primers: DIGl2 (5’-AAG CAT ATC ACT AAG CGG-3’) and 1500R (5’-GCT ATC CTG AGG GAA ACT TCG-3’) ...of 28S ...
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Vargas Ramirez, Sergio (2012): Evolution and ecology of antarctic sponges. Dissertation, LMU München: Fakultät für Geowissenschaften
... (i.e. 28S rDNA and COI) for all non-monotypic cladorhizid genera and a new species of Phelloderma collected in the Southern Ocean, and assess alternative hypotheses on the position of Abyssocladia advanced ...
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Vol 69, No 3 (2017)
... agarose gels. Negative control reactions without DNA were included in all sets of amplifications to monitor for potential contamination by exogenous DNA. PCR products were purified using the NucleoSpin® Extract II PCR ...
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First evidence of Besnoitia bennetti infection (Protozoa: Apicomplexa) in donkeys (Equus asinus) in Belgium
... Ribosomal DNA ITS1 sequences are only available for B. bennetti isolated from donkeys in the USA. No ITS1 sequence variation was found between Belgian and USA samples with the same T insertion at position 148 (Add- ...
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Phylogenetic Utility of Two New Nuclear Genes, Opsin and CAD, within the stiletto flies (Diptera: Therevidae)
... Phylogenetic relationships based on combined analysis of 28S rDNA, EF-1 α , opsin and CAD gene sequences of 26 taxa in 24 genera, excluding third nucleotide positions. The tree was estim[r] ...
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Identification and species-typing of wood rotting fungi using melting curve analysis
... three rDNA, the 18S, 28S, and 5.8S rDNA; inter transcribed spacer (ITS) regions; and a large intergenic spacer (IGS) region ...another rDNA, the 5S, may be present within the IGS. The ...
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Identification of Dermatophyte Species by 28S Ribosomal DNA Sequencing with a Commercial Kit
... The 28S rDNA is the target of choice in the development of a method for rapid identification of dermatophytes with high specificity and ...with rDNA sequences from the EMBL GenBank ...essentially ...
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Candida inconspicua and Candida norvegensis: New Insights into Identification in Relation to Sexual Reproduction and Genome Organization
... the 28S rDNA, and the elongation factor 1 ␣ (EF-1 ␣ ) gene, either directly or following cloning, of 13 clinical isolates and 9 reference strains belonging to the 5 species included in the Pichia cactophila ...
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Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain
... Based on data collected thus far, S. lutrae shows intra- specific diversity only within ITS1, while S. arctica is characterized by some intraspecific variability within 28S rDNA, ITS1, cox1 and rpoB. The ...
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Heligmosomoides neopolygyrus Asakawa & Ohbayashi, 1986, a cryptic Asian nematode infecting the striped field mouse Apodemus agrarius in Central Europe
... Heligmosomoides collected from A. agrarius in Poland appeared at first sight to belong to H. polygyrus, the common species recorded from Apodemus throughout Europe. The worms were red, slender and strongly coiled, with ...
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Isolation and Characterization of Two Rare Mucoralean Species with Specific Habitats
... Phylogenetic tree based on neighbor-joining analysis of 28S rDNA sequences for Gilbertella.. persicaria CNUFC-GWD3-9, G.[r] ...
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Intermediate hosts of the trematode Collyriclum faba (Plagiochiida: Collyriclidae) identified by an integrated morphological and genetic approach
... Only limited information can be retrieved from studies of species closely related to C. faba. Some classification systems suggest the two Cortrema spp. as members of the family Collyriclidae but the recently published ...
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The application of DNA sequence data for the identification of benthic nematodes from the North Sea
... and 28S rRNA genes ...the 28S rDNA represents one of the most suitable and promising markers for a valid nematode identification, which can be used to delimitate even closely related species (De Ley ...
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Karyotype Characterization of Nine Periwinkle Species (Gastropoda, Littorinidae)
... 5S rDNA probes were labeled by supplementing the PCR mixtures either with 20 µM biotin-16-dUTP (Roche Applied Science, Penzberg, Germany) or 5 µM digoxigenin-11-dUTP (10 × DIG Labeling Mix, Roche Applied Science), ...
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