293T cells
Incorporation of podoplanin into HIV released from HEK 293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC 2
... knock-down cells. For this, the cells were transfected with env-deficient HIV-1 proviral DNA (for augmented bio- safety), the supernatants concentrated by size-exclusion filtration and virions pelleted by ...
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Development of a Model System for Tick Borne Flavivirus Persistence in HEK 293T Cells
... kidney) cells differed from our LGTV system in that cells surviving the initial lytic crisis reattained con- fluence but underwent successive lytic crises ...HEK 293T cells, observation of the ...
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Transcriptome Analysis Reveals a Signature Profile for Tick Borne Flavivirus Persistence in HEK 293T Cells
... (HEK) 293T cells in vitro results in the death of >95% of infected cells by day ...surviving cells to repopulate and become persistently infected for extended peri- ods of ...HEK ...
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S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T Cells
... Human Reelin containing 5th and 6th repeats (R5-R6), a part of 8 th repeat and the CTR which were amplified from cDNA of K562 and HEPG2 cells and cloned into a mammalian expressional plasmid (pVP22/myc-His). ...
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Apolipoprotein E Codetermines Tissue Tropism of Hepatitis C Virus and Is Crucial for Viral Cell-to-Cell Transmission by Contributing to a Postenvelopment Step of Assembly
... those cells and tissues that express ...nonliver cells as well as nonhuman cells permissive to HCV cell entry ...liver cells, has emerged as an important cell type-specific enhancer of ...
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The Sheep Tetherin Paralog oBST2B Blocks Envelope Glycoprotein Incorporation into Nascent Retroviral Virions
... in 293T cells constitutively expressing ...in 293T-cont and 293T/B-HA cells were observed when a small amount ...in 293T cells stably expressing oBST2B-HA protein whose ...
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Characterisation of the mouse glycosyl phosphatidylinositol-phospholipase D (GPI-PLD) gene
... transfected cells with antibodies recognising proteins exclusively localised in ...BHK cells (Luetterforst et ...of 293T cells with a monoclonal antibody against galactosyltransferase-1 ...
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A human embryonic kidney 293T cell line mutated at the Golgi mannosidase II locus
... HEK 293T Lec36 ...Lec36 cells. s19A expressed alone in HEK 293T cells exhibits some Endo H sensitivity ...HEK 293T Lec36 cells is largely Endo H sensitive ...HEK 293T ...
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NHEJ pathway is involved in post integrational DNA repair due to Ku70 binding to HIV 1 integrase
... co-transfect cells with CRISPR/Cas9 ...co-transfecting 293T cells with the spCas9 expression plasmid (Addgene), the gRNA coding plasmid and a respective ...+ cells were sorted out after ...
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Borna Disease Virus P Protein Affects Neural Transmission through Interactions with Gamma-Aminobutyric Acid Receptor-Associated Protein
... assay. 293T cells were cotransfected with plasmids pCMV-GABARAP expressing Flag-tagged GABARAP and pCDNA-P express- ing P ...transfected cells were lysed by freeze-thaw cycling in a buffer containing ...
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H5-Type Influenza Virus Hemagglutinin Is Functionally Recognized by the Natural Killer-Activating Receptor NKp44
... CHO cells. Yet 293T cell-produced NKp44-Ig also bound recombinant ...treated 293T cell-produced NKp44-Ig with neuraminidase as described in reference ...CHO cells and the enhanced affinity of ...
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Human and murine APOBEC3s restrict replication of koala retrovirus by different mechanisms
... in cells as well as media in dose-dependent manners ...in 293T cells co-transfected with the pKoRV522 and M-MuLV expression vector p63-2 ...co-transfected cells was largely impaired by mA3 ∆ ...
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Functional Differences between Human and Bovine Immunodeficiency Virus Tat Transcription Factors
... Human 293T cells were cotransfected with either the pHIV/bTAR/CAT or pHIV/SLIIB/CAT indicator construct together with an effector plasmid encoding bTat or the Rev-bTat or Rev-C38S fusion ...in 293T ...
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Heterogeneous susceptibility of circulating SIV isolate capsids to HIV interacting factors
... HEK-293T cells, VSV-G pseudotyped eGFP-expressing chimeric HIV MACA or CA virions were produced and we verified the correct p55Gag pro- teolytic cleavage by detecting the presence of CAp24 in virus pellets ...
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The effect of simvastatin on lipid droplets accumulation in human embryonic kidney cells and pancreatic cancer cells
... MiaPaCa-2 cells was in general higher as compared to that in HEK-293T cells (Figure 2a ...MiaPaCa-2 cells there were not detectable changes in LDs amounts after 24 hours of ...MiaPaCa-2 ...
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The Interferon Antagonist ML Protein of Thogoto Virus Targets General Transcription Factor IIB
... case, an excess of TFIIB might abrogate the IFN antagonism of ML. Therefore, we tested the effect of transfected ML on IRF3-dependent promoter activity in the presence and ab- sence of overexpressed TFIIB with the help ...
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HIV 1 replication in cell lines harboring INI1/hSNF5 mutations
... for 293T and p = ...and 293T cells in the presence of INI1 (Figure ...MON cells resulted in further increase (about 10 fold *p = ...in 293T cells (p = ...MON cells, in ...
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Distinct Regulation of Hepatitis B Virus Biosynthesis by Peroxisome Proliferator-Activated Receptor γ Coactivator 1α and Small Heterodimer Partner in Human Hepatoma Cell Lines
... kidney 293T cells in the presence of nuclear recep- tors indicated that neither RXR ␣ /FXR ␣ nor LRH1 alone might be controlling the majority of viral RNA and DNA synthesis in the HepG2 cells ...
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ORF3 Protein of Hepatitis E Virus Is Not Required for Replication, Virion Assembly, or Infection of Hepatoma Cells In Vitro
... (A549) cells could also be infected with HEV, albeit with a very low efficiency ...nonhepatic cells, we transfected 10-3 cells and CaCo-2 cells (human intestinal cells) in one case or ...
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Fundamental Contribution and Host Range Determination of ANP32A and ANP32B in Influenza A Virus Polymerase Activity
... Briefly, 293T cells in 6-well plates were transfected with ...(GFP)-positive cells were sorting by fluorescence-activated cell sorting (FACS) at 48 h posttransfection, and then monoclonal knockout cell ...
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