2A Proteinase
Encapsidation and serial passage of a poliovirus replicon which expresses an inactive 2A proteinase.
... of 2A in ...the 2A proteinase, in which a cysteine amino acid at position 109 was changed to a serine (C109S), resulted in inactivation of the viral protease (8, 31, ...of 2A in viral ...
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Determinants of substrate recognition by poliovirus 2A proteinase.
... The mechanism which limits proteolytic processing to specific sites has not been fully characterized, but by analogy with other viral proteinases 6, 7, 23, it is likely that substrate re[r] ...
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Characterization of poliovirus 2A proteinase by mutational analysis: residues required for autocatalytic activity are essential for induction of cleavage of eukaryotic initiation factor 4F polypeptide p220.
... A second function of 2APro in poliovirus infection of HeLa cells is in inducing cleavage of the 220-kDa polypeptide p220 component of eukaryotic translation initiation factor eIF-4F 8, 9[r] ...
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Conformational Plasticity of the 2A Proteinase from Enterovirus 71
... the 2A pro interaction with the substrate, however, in which the binding of a side chain from the substrate into the cleft would alter the dynamics of the struc- ture to favor the open conformation and to make the ...
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Structure and Dynamics of Coxsackievirus B4 2A Proteinase, an Enyzme Involved in the Etiology of Heart Disease
... Members of the picornavirus family include several common and important pathogens of humans, e.g., poliovirus, hepatitis A virus, rhinovirus (common cold), and coxsackievirus. Infec- tion of heart muscle myocytes with ...
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A poliovirus minireplicon containing an inactive 2A proteinase is expressed in vaccinia virus-infected cells.
... The transfection of the chimeric HIV-2-gag-poliovirus genome containing the 2A mutation into poliovirus-vaccinia virus coinfected cells resulted in the expression and partial processing [r] ...
9
The eIF4G-eIF4E complex is the target for direct cleavage by the rhinovirus 2A proteinase.
... termediates, the cap-binding protein complex eIF4F. Because previous studies were performed with eIF4F purified from rabbit reticulocyte lysate, it could be argued that this complex contained some trace amounts of other ...
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Relationship of p220 cleavage during picornavirus infection to 2A proteinase sequencing.
... Thus, despite the absence of the proposed proteinase sequence that is conserved in the 2A genes of the other picornaviruses that induce p220 cleavage in their infected host cells, FMDV d[r] ...
8
Foot-and-mouth disease virus Lb proteinase can stimulate rhinovirus and enterovirus IRES-driven translation and cleave several proteins of cellular and viral origin.
... Lb proteinase, as outlined ...Lb proteinase has a much broader specificity than do the rhino- virus and enterovirus 2A ...Lb proteinase but not with the 2A proteinases ...CVB4 2A ...
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Antiviral Effects of Pyrrolidine Dithiocarbamate on Human Rhinoviruses
... Most of the inhibitors of picornavirus infection act at an early stage of infection, i.e., during virus binding, internaliza- tion, and/or uncoating. Only very few of the known inhibitors are effective late in the viral ...
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Increased expression of Candida albicans secretory proteinase, a putative virulence factor, in isolates from human immunodeficiency virus positive patients
... for proteinase activity on agar plates containing bovine hemoglobin (Sigma, Deisenhofen, Germany) as the incorporated substrate ...aspartyl proteinase was quantitated essentially as described previously ...
7
Mutational analysis of tobacco etch virus polyprotein processing: cis and trans proteolytic activities of polyproteins containing the 49-kilodalton proteinase.
... The locations of the 49-kDa' proteinase-mediated cleavage sites flanking the 71-kDa cytoplasmic pinwheel inclusion protein, 6-kDa protein, 49-kDa proteinase, and 58-kDa putative polymera[r] ...
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Characterization of the Epstein-Barr virus proteinase and comparison with the human cytomegalovirus proteinase.
... the proteinase domain following cleavage from the polyprotein yielded a product which has the expected size, suggesting that the predicted release site was ...
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Proteinase 3 A distinct human polymorphonuclear leukocyte proteinase that produces emphysema in hamsters
... dramatic bullous emphysema developed in animals treated with pool 4. The esterase from pool 4, designated proteinase 3 (PR-3) was purified, characterized in vitro, and tested for its ability to cause emphysema. ...
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Evidence for antimicrobial activity associated with common house spider silk
... Our study of T. domestica silk shows that the anti- microbial activity against B. subtilis is reduced or negated after digestion with Proteinase K. The reduction of antimicrobial properties after treatment with ...
6
Characterization of a Torovirus Main Proteinase
... two proteinase domains were found: a cysteine pro- teinase within the N-terminal half of pp1a and a more C-termi- nally located serine proteinase ...
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Deubiquitinating Function of Adenovirus Proteinase
... Both LaggH and nonbiotinylated Ubal were used to com- pete for the retrieval of proteins from Ad2-infected cells on biot-Ubal column. Pretreatment of the cell lysate with nonbi- otinylated Ubal or LaggH before incubation ...
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Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites.
... To further refine the map of the HCV nonstructural proteins and to determine the sequence at the four potential NS3 proteinase cleavage sites, the N-terminal sequences of NS4A, NS4B, NS5[r] ...
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2A induced ribosome stalling
... for 2A would be ...no 2A activity with mutated [NPG], supports the theory that a ribosome residue within the vicinity of the PTC is responsible for the stalling mechanism, and through a relay to PTC ...
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A study of the 2A region of aphthoviruses
... Plasmid constructs, in which the codons encoding the -KLAG- motif were replaced by those for -IH-, -HI- and -RI-, were made by using the PCR to amplify a mutated 2A region to replace the wild-type 2A region ...
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