2D electrophoresis
2D-electrophoresis and multiplex immunoassay proteomic analysis of different body fluids and cellular components reveal known and novel markers for extended fasting
... The platelets and saliva were analyzed in laboratory 1, PBMC and urine in laboratory 2 and depleted plasma in laboratory 3. Each of the three laboratories ran one gel per sampling time point, the 4 overnight fasting time ...
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Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry
... The fractionation of proteins by two-dimensional electrophoresis O'Farrell using isoelectric focusing in pH gradient (IPG- PAGE). When IPG-PAGE using a modification of the two-dimensional electrophoretic ...
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Global Proteomics of the Extremophile Black Fungus Cryomyces antarcticus Using 2D Electrophoresis
... All 2DE experiments were carried out in triplicates. For the analysis 13 cm strips non-linear pH 3 - 10 were used (GE-Healthcare Immobiline DryStrip pH 3 - 10 NL, 13 cm, Sweden). Using protein concentration value we ...
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Two Dimensional Electrophoresis pdf
... quantities. 2D elec- trophoresis represents the core methodology of the new, technology-driven science ...of 2D electrophoresis for the detection of variable protein levels between diseased and ...
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Comparative proteomic analysis of metabolically labelled proteins from Plasmodium falciparum isolates with different adhesion properties
... brane extraction reagent 2 (Sigma) 5 M urea, 2 M thiou- rea, 40 mM Tris base, 2% CHAPS, 2% SB3-10 and 25 μ l/ ml of tributylphosphine (TBP) were added to help solubi- lize the erythrocyte membranes, and then solubilized ...
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2D-DIGE proteomic analysis identifies new potential therapeutic targets for adrenocortical carcinoma
... a 2D-DIGE approach to the analysis of ACC compared with normal adrenal, resulting in identification of a protein pattern specifically overexpressed in ...applied 2D-DIGE, but rather narrow range isoelectric ...
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Application of proteomics to identify the target molecules involved in Lonicera japonica induced photokilling in human lung cancer CH27 cells
... of 2D electrophoresis, most of protein spots are more intense in control cell-loaded gels than in the photoactivated Lonicera japonica-treated cell-loaded gels in this ...
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The C-terminal intact forms of periostin (iPTN) are surrogate markers for osteolytic lesions in experimental breast cancer bone metastasis
... Proteins extracted from the serum, and bone and soft tissues lung and heart of wild- 190 type and Postn-/- mice were then subjected to 2D electrophoresis, transferred to nitrocellulose m[r] ...
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Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry
... the 2D-gels compared to the unlabelled ...following 2D-electrophoresis with another agent such as silver nitrate or SYPRO Ruby is necessary for spot picking and cutting, especially for lower ...
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Three Isoforms of Cyclophilin A Associated with Human Immunodeficiency Virus Type 1 Were Found by Proteomics by Using Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
... stained. 2D SDS-PAGE standards (Nippon Bio-Rad Laboratories) were used for the reproducibility of 2D ...of 2D electrophoresis patterns of different samples. The 2D gel pattern shows the ...
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Recent advances in the compound-oriented and pattern-oriented approaches to the quality control of herbal medicines
... under Matlab [85]. Gong et al. developed a series of che- mometric methods to extract information from finger- prints [24,86,87]. Xu et al. used target peak alignment (TPA) to correct the retention time shifts and ...
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Increase in local protein concentration by field-inversion gel electrophoresis
... gel electrophoresis (PFGE) is an elegantly simple and universally accepted technique for the separa- tion of large DNA molecules ...gel electrophoresis (OFAGE) technique ...gel electrophoresis ...
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Secretome analysis of rice suspension-cultured cells infected by Xanthomonas oryzae pv.oryza (Xoo)
... gel electrophoresis (2D-DIGE) coupled with MALDI-TOF/TOF mass spectrometry (MS), to investigate secreted proteins in Nipponbare embryo cell suspension culture infected by ...
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Contributions of mass spectrometry based proteomics to defining cellular mechanisms and diagnostic markers for systemic lupus erythematosus
... two-dimensional electrophoresis and LC- MS/MS with an ESI-LCQ instrument to obtain higher sensitivity for low abundance ...two-dimensional electrophoresis rat cerebral lysate ...
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Systematic evaluation of immune regulation and modulation
... gel electrophoresis; ACK: Ammonium chloride potassium; AE: Adverse event(s); ALC: Absolute lymphocyte count; CAR: Chimeric antigen receptor(s); CRC: Colorectal cancer; CRP: C-reactive protein; CRS: Cytokine ...
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Original Article Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach
... Healthcare). This imager is a charge-coupled device-based instrument that enables scan- ning at different wavelengths for Cy2-, Cy3-, and Cy5-labeled samples. Gel analysis was performed using DeCyder 2D ...
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Interactions by 2D Gel Electrophoresis Overlap (iGEO): a novel high fidelity approach to identify constituents of protein complexes
... the native form. Hence, the altered characteristics of the tagged protein may lead to false discovery of spurious interactions, or to the exclusion of bona fide interactions [28,29]. Given these considerations, when we ...
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Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease
... in-gel electrophoresis; 2D PAGE: Two dimensional polyacrylamide gel electrophoresis; ANOVA: Analysis of variance analysis; CHAPS: 3-((3-Cholamidopropyl) dimethylammonio)-1- propanesulfonate; CID: ...
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ELECTROPHORESIS pdf
... power supply can be as simple as a 12 V battery, but is usually a mains electricity transformer delivering 100 } 500 V DC. The required voltage is selected by a digital potentiostat combined with a polarity switch, the ...
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METAL SEPARATION FROM WASTEWATER BY ELECTRO-KINETICS METHOD- A REVIEW
... The the referred papers shows that maximum separation is achieved by capillary electrophoresis technique. Without chemicals and reagents separation metal separation is not possible. High voltage is necessary foe ...
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