3D cell culture models
Cellular effects of paclitaxel-loaded iron oxide nanoparticles on breast cancer using different 2D and 3D cell culture models
... controls containing the respective amount of drug-free SPIONs. The cellular confluency within the samples was calculated from the images taken hourly for 7 days. Notably, due to experimental prerequisites, the long-time ...
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The fibroblast Tiam1 osteopontin pathway modulates breast cancer invasion and metastasis
... tissue models with a range of technical complexity and biologically relevant tissue ...of 3D spheroid co-cultures containing mammary fibroblasts. Co-culture of human breast cancer cells with ...
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Development of an in vitro cell-sheet cancer model for chemotherapeutic screening
... vitro 3D culture models that mimic the epithelial tumour microenvironment in vivo are lacking; therefore, we developed a new 3D epithelial cancer model using in vitro cell-sheet ...
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Automated tracking of tumor-stroma morphology in microtissues identifies functional targets within the tumor microenvironment for therapeutic intervention
... co-culture models requires distinction of the two ...traceable cell lines that stably expressed fluorescent markers (GFP; fibroblast and dsRed; tumor cells) were ...the 3D co-cultures, we ...
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Gill cell culture systems as models for aquatic environmental monitoring
... the culture of a RTgill-W1 cell line and showed using a cell viability assay that this system could be used for toxicity ...in 3D to better mimic organ structure and ...for cell ...
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Generating an in vitro 3D cell culture model from zebrafish larvae for heart research
... vitro models are widely used in biomedical, toxicological and pharmacological research as they provide a simplified test system whilst aligning with the principles of reduction in animal ...stem cell ...
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The 3D Cell Culture System in the Study of Tumor Applications and Prospects
... and 3D culture on some as- pects of cell growth, it is clear that 2D culture has an inevitable limitation in ap- plication, especially in the study of microenvironment and cell ...
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3D culture models of Alzheimer’s disease: a road map to a “cure-in-a-dish”
... achieve 3D structures that can facili- tate interstitial compartments for Aβ deposition ...of 3D brain orga- noid cultures using AD patient-derived ...generate 3D brain organoids/neuro-spheroids that ...
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Epigallocatechin-3-gallate (EGCG) inhibits the migratory behavior of tumor bronchial epithelial cells
... inhibit cell migration or inva- sion in liver cancer cells [5], glioblastoma cells [3], vascu- lar smooth muscle cells [35,36], pancreatic stellate cells [37] or during angiogenesis ...vitro models similar ...
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Use of hydrogel scaffolds to develop an in vitro 3D culture model of human intestinal epithelium
... The human intestinal cell lines: Caco-2 and HT29-MTX cells have been used.. extensively in 2D and 3D cell cultures as simple models of the small intestinal.[r] ...
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3D skin models for 3R research: The potential of 3D reconstructed skin models to study skin barrier function
... of 3D skin models in skin barrier research, we should be aware that experi- mental heterogeneity and the wide variety in types of skin models based on cell sources, cell types, dermal ...
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Cell Dynamics in Three-dimensional (3D) Culture Environments
... of cell-containing aqueous droplets can be deposited on substrate via using thermal or acoustic forces to fabricate construct [105, 106, 193], but rapid post solidification of bio-ink is required and resolution ...
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Microfluidic co-culture of pancreatic tumor spheroids with stellate cells as a novel 3D model for investigation of stroma-mediated cell motility and drug resistance
... cancer models [59], providing a rationale for therapeutic targeting of ...greater cell motility along with significantly higher vimentin and lower E-cadherin expression ...
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Neuronal and astrocytic interactions modulate brain endothelial properties during metabolic stresses of in vitro cerebral ischemia
... a 3D co-culture model of brain endothelium with neurons and astrocytes in vitro reflecting an intimate neurovascular and gliovascular interactions in ...the 3D co-culture models in ...
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Three dimensional cell culture: The missing link in drug discovery
... the 3D scenario as the core of the spheroids are hypoxic due to the limited ability of oxygen to diffuse to the centre of the ...in 3D cells due to the higher levels of hypoxic cells than in 2D, resulting ...
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2193-1801-3-80.pdf
... on 3D gel substrates. In 1985, Sertoli cell and germ cell survival and differentiation were accomplished by using a 3D ...using 3D systems (Benton et al. 2009). 3D systems ...
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SAHA induced TRAIL sensitisation of Multiple Myeloma cells is enhanced in 3D cell culture
... Biomimetic culture systems may allow for in vitro tumour modelling to exploit cancer cells' dependency on these conditions ...used 3D tumor models based on alginate gel to in-part mimic the hypoxic ...
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Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles
... 3-dimensional cell cultures modeling avascular tissue when compared to standard poly(ethylene glycol) (PEG)-coated ...tumor cell cultures were evaluated using silver-enhancement staining and hyperspectral ...
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Phenotypic screening with 3D cell-based assays
... ing and this inhibition was presented graphically as a heat map from yellow (no inhibi- tion) to dark blue (potent inhibition) (figure 4A). Our validation screen showed that the activity of most of the active compounds ...
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3D Bioprinting of mature bacterial biofilms for antimicrobial resistance drug testing
... 2D culture (fi gure 4 ( a )) ...in 3D bioprinted MRSA and MSSA biofilm cul- ture models (figure ...of culture wells and correlated with measurements of absorbance of light through treated ...
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