96-Well plate
HIGH THROUGHPUT LC MS/MSMETHOD FOR THE QUANTITATION OF LINAGLIPTIN IN HUMAN PLASMA BY SOLID PHASE EXTRACTION USING 96 WELL PLATE FORMAT
... ABSTRACT: High-throughput Liquid chromatography–mass spectrometry method has been developed and validated for the quantification of Linagliptin in human plasma using Linagliptin D4 as an internal standard (ISTD). ...
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Original Article Regulatory effect of Nemo-like kinase on nasopharyngeal carcinoma
... into 96-well plate at 3000 cells per well and were randomly divided into scramble RNA, NLK siRNA and control group (N=5 ...each well for vortex until complete resolving of violet crys- ...
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Original Article Interleukin-1 receptor-associated kinase 4 overexpression attenuates angiotensin II-induced cardiomyoblast hypertrophy
... per well were added to H9c2 cardiomyoblasts in 96-well ...each well of the 96-well plate and then incubated for an additional 7 ...
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Drug resistance profile and biofilm forming potential of Pseudomonas aeruginosa isolated from contact lenses in Karachi-Pakistan
... microtiter plate (non-tissue culture treated, ...the well were aspirated out and the wells were washed thoroughly with ...each well. After- wards, the stain was removed and the plate was ...
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Effect of plant hormones on the production of biomass and lipid in microalgae
... Dimethyl sulfoxide (DMSO) is one of the organic solvents which is able to impose stress on test organisms (El Jay, 1996). For establishing a technique to detect the inhibitory effect of DMSO on the growth kinetics of ...
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DPPH antioxidant activity, total phenolic and total flavonoid content of different part of Drumstic tree (Moringa oleifera Lam )
... modification.96-well plate was used, for the assay, where by 60 µL of Moringa extract diluted in DMSO was mixed with 200 µL of DPPH in methanol ...per well. The plate was placed in the ...
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← Return to Article Details SYNTHESIS OF TETRASUBSTITUTD IMIDAZOLES CONTAINING INDOLE AND THEIR ANTIUREASE AND ANTIOXIDANT ACTIVITIES
... each well in the 96-well plate followed by the addition of 10 µl of sample solution and 25 µl of enzyme solution ...each well and incubation continued at 37ºC for further 10 min and ...
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Visible Thrombolysis Acceleration of a Nanomachine Powered by Light Driving F0F1 ATPase Motor
... the 96-well plate at a density of 5000 cells per well in 100 uL ...each well, and the plate was left standing for an additional 2 h at 37 ...
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A Functional Interaction between Herpes Simplex Virus 1 Glycoprotein gH/gL Domains I and II and gD Is Defined by Using Alphaherpesvirus gH and gL Chimeras
... a 96-well plate were transfected over- night with plasmids encoding FLAG (F)-tagged gH from HSV-1 or SaHV-1, FLAG-tagged gH chimeras, or empty vector, together with wild-type HSV-1 (black bars) or ...
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Determination of aflatoxins in food products by the ELISA method
... each well of 96 well plate coated with antibodies to aflatoxin M 1 and incubated for 60 min at a room ...the plate was emp- tied and washed five times, 100 µl of the aflatoxin M 1 ...
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... to 96-well plate and incubated for 24 hour at 37°C, then treated with different concentration of pomegranate seed oil (100-1200 μg/mL) and the volume of the each well was 100 ...each ...
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Isolation of Cell Lines That Show Novel, Murine Leukemia Virus-Specific Blocks to Early Steps of Retroviral Replication
... B Cells 1 ⫻ 104/well of a 96-well plate of WT CHO-K1 or the indicated cell lines engineered to express TVA800 were infected with either 5 ⫻ 104 IU LTU of the MLV vectors MMp-nls-LacZ[VSV[r] ...
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High throughput biodegradation screening test to prioritize and evaluate chemical biodegradability
... previous 96-well plate biodegradation work. 27 A whole plate was used for each inoculum dilution ...(i.e., 96 replicates ...a plate centrifuge (CL30, ThermoFisher Scienti fi c, ...
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LicT Modulates Biofilm Formation of Streptococcus pyogenes
... Streptococcus pyogenes frequently causes purulent infections in humans. Biofilm formation is an important virulence property of S. pyogenes because of decreased susceptibility of bacteria to an- tibiotic treatment. ...
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Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes
... The lipidic cubic mesophase has been used to crystallize important membrane proteins for high-resolution structure determination. To date, however, no integral membrane enzymes have yielded to this method, the in meso. ...
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Combinatorial microfluidic droplet engineering for biomimetic material synthesis
... four plate wells ...right well). The fourth well (Fig. 1, B and D, bottom left well) includes a metal tube, which is introduced to the plate by first puncturing the bottom of the ...
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Modification of Membrane Fluidity by Gravity
... is well accepted since long for a wide range of biological sys- ...as well as that of cell membranes, is gravity dependent, using a multipurpose 96-well plate reader in the fluorescence ...
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Development of a high throughput cell based 384 well influenza A quantification assay for interpandemic and highly pathogenic avian strains
... the plate format. Both 96-well and 384-well plate formats tested were found to be repeatable, precise, and ...384-well plate appears to be more accurate, precise and ...
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Transport of Solid Bodies Through Fluid Interfaces: From Flying Submarines to Cancer Spheroids.
... the plate, however some possible explanations include surface tension effects, surface adsorption of biomolecules, friction, and electrostatic ...off-the-shelf 96-well plates which would not have ...
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Respiration activity monitoring system for any individual well of a 48-well microtiter plate
... total 96 pneumatic valves and 48 optical fibers, providing two valves and one optical fiber for each ...and well. The well-to-well deviation is investigated by 48 identical Escherichia coli ...
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