absolute quantification
Absolute Quantification of Enzymes in the Monolignol Biosynthetic Pathway under Transgenic Effect by Mass Spectrometry.
... We calculated the percentage difference for all of the target proteins within all the enzyme families. We can see that CAD1, as its target gene was knocked down in level 2, decreased about 99% compared to the wild type. ...
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Recent developments in quantitative SERS : moving towards absolute quantification
... In all areas of science there is a need for absolute quantification of analytes of interest. This may be within the context of environmental science where the level of a toxicant polluting an ecological ...
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Absolute Quantification of Individual Biomass Concentrations in a Methanogenic Coculture
... the absolute quantification of individual biomass concentrations in methanogenic microbial ecosystems which has so far impaired the calculation of biomass specific conversion rates and thus model ...
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Absolute quantification of the pretreatment PML-RARA transcript defines the relapse risk in acute promyelocytic leukemia
... the quantification by RT-qPCR of the PML- RARA transcript during the postconsolidation phase is a valuable prognostic tool to predict APL relapse [11, 16], the PML-RARA molecular burden at diagnosis has not been ...
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A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance
... the absolute quantification of even low abundance proteins from the folate metabolic pathway within unfrac- tionated cell ...to absolute quantification of malarial ...Such ...
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Absolute quantification of cell-free microRNAs in cancer patients
... accurate absolute quantification of specific cell-free miRNAs, working in every step with constant ...miRNA quantification, because of its higher sensitivity and reduced variability in low- abundance ...
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Exploring Fundamental Aspects of Proteomic Measurements: Increasing Mass Measurement Accuracy, Streamlining Absolute Quantification, and Increasing Electrospray Response
... The absolute quantification of a protein from its digestion products dates back to Barr ...for absolute quantification of a European Community Bureau of Reference (BCR) certified ...
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Simultaneous and absolute quantification of nucleoside triphosphates using liquid chromatography–triple quadrupole tandem mass spectrometry
... and absolute quantification method for eight nucleoside triphosphates using liquid chromatography – triple quadrupole tandem mass spectrometry and hydrophilic interaction ...
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Absolute quantification of endogenous angiotensin II levels in human plasma using ESI-LC-MS/MS
... for absolute quantification of the native angiotensin II in human plasma, we decided to isolate and fragment both the native and stable isotope labeled peptide in the same ...
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Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells
... For quantification of genomic DNA copy number in the mutant plants relative to wild-type plants, 1 μl of protein extract of wild-type plants (diluted 1:10, 1:20, 1:40, 1:80 or 1:160) was added to the PCR ...
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Fast Absolute Quantification of In Vivo Water and Fat Content with Magnetic Resonance Imaging
... To avoid the limitations mentioned above in absolute quantification of water and fat content, we propose an extension of the works of Shah et al. and Hu et al. In this proposed method, the IDEAL sequence is ...
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Absolute Quantification of Proteins and Metabolites by Mass Spectrometry: Fundamental and Novel Applications towards Development of a Systems Level Assay for Lignin Biosynthesis.
... from quantification if the relative peak areas (measured as ratios) of the three transitions deviated by more than 20% from that of the standard compounds (see Table ...
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Absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis: a cohort study
... In summary, absolute ascites MP levels represent a novel independent prognostic factor in patients with decom- pensated liver cirrhosis. Note that the detection of abso- lute MP levels from ascites samples was ...
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Absolute quantification of somatic DNA alterations in human cancer
... Author Contributions: M.M. posed the concept of using allelic copy number analysis to assess tumor genomic purity. G.G. and S.L.C. conceived and designed the analysis. S.L.C. designed and implemented the ABSOLUTE ...
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A detailed analysis of next generation sequencing reads of microRNA expression in Barrett’s Esophagus: absolute versus relative quantification
... at absolute quantification and second, there was a high degree of correlation between NGS and PCR in fold changes for differentially expressed miRNAs across the GERD and BE ...for absolute versus ...
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Investigation of strategies to protect against harmful bacteria – mucosa interaction in crohn’s disease and other diarrhoeal diseases
... accurate quantification could not be achieved, particularly in the tissues with high bacterial ...using absolute quantification, and are categorical ...an absolute quantification assay ...
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Novel Approach for Quantitative Measurement of Matrix Metalloprotease 1 (MMP1) in Human Breast Cancer Cells Using Mass Spectrometry
... and quantification of low abundance growth factors and regulators in complex biological samples still present a challenging task in analytical ...MS. Absolute quantification of MMP1 was conducted ...
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Mll partial tandem duplication induces aberrant Hox expression in vivo via specific epigenetic alterations
... Absolute quantification by SYBR similar histone modifications at the Hoxa10 green real-time PCR shows a significant increase at the Hoxa9 promoter in histone H3/H4 promoter, suggesting i[r] ...
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dpcr for the absolute diagnostic quantification of mirnas in human colon cancer stool
... an absolute quantitative digital PCR on the above fifteen stool samples from stages 0-4, using total small RNA that was extracted by immunocapture, followed by reverse transcription (RT), and finally using an ...
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PubMedCentral-PMC4931884.pdf
... achieve absolute quantification using an LC-MRM-based targeted proteomic approach, two types of standards are typically employed, recombinant proteins of known concentration or synthetic signature peptides ...
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