Acanthamoeba spp.
Apoptosis of Primary-Culture Rat Microglial Cells Induced by Pathogenic Acanthamoeba spp.
... It is well known that pathogenic Acanthamoeba spp. have CPE on various cells in vitro and in vivo. With the destruction of rat neuroblastoma cells by Acanthamoeba spp., the exten- sion of a ...
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Isolation and genotyping of Acanthamoeba spp from Acanthamoeba meningitis/ meningoencephalitis (AME) patients in India
... Previously Acanthamoeba spp. was divided into three groups based on morphological characteristics i.e. cyst size, shape and growth temperature conditions which were confusing [12]. However with the ...
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Occurrence of Potentially Pathogenic Bacterial-Endosymbionts in Acanthamoeba Spp.
... DNA extraction was performed using modi- fied phenol-chloroform method according to our previous study (14). For genotyping of Acanthamoeba spp. PCR analysis was done us- ing genus-specific primer pairs ...
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Molecular Identification of Free-Living Amoebae (Naegleria spp., Acanthamoeba spp. and Vermamoeba spp.) Isolated from Unimproved Hot Springs, Guilan Province, Northern Iran
... Acanthamoeba spp., Naegleria fowleri and Bal- amuthia mandrillaris are the most commonly reported causes in the world (2). Other genera in this group, including Sappinia diploidea, Ver- mamoeba and ...
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Multiplex Real Time PCR Assay for Simultaneous Detection of Acanthamoeba spp , Balamuthia mandrillaris, and Naegleria fowleri
... Triplex real-time PCR. For the detection of Acanthamoeba spp., primers AcantF900 (5⬘-CCC AGA TCG TTT ACC GTG AA-3⬘) and AcantR1100 (5 ⬘ -TAA ATA TTA ATG CCC CCA ACT ATC C-3 ⬘ ) were used to amplify ...
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Molecular detection of Acanthamoeba spp , Naegleria fowleri and Vermamoeba (Hartmannella) vermiformis as vectors for Legionella spp in untreated and solar pasteurized harvested rainwater
... EMA-qPCR assays were then performed for all unpasteurized and tank water samples pasteurized at 68 °C, 74 °C, 84 °C and 93 °C to determine whether Le- gionella and the FLA’s were viable. Although viable V. vermiformis ...
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Occurrence and genetic characterisation of Acanthamoeba spp from environmental and domestic water sources in Queen Elizabeth Protected Area, Uganda
... species. Acanthamoeba sequence types have been previously grouped from T1 to ...partial Acanthamoeba sequences belonging to sequence T-genotype groups T1, T2, T4, T5, T6 and T11, with only two identical ...
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Combating Acanthamoeba spp cysts: what are the options?
... soil. Acanthamoeba exist in two forms: (i) an active trophozoite stage and (ii) a resistant double-walled cyst stage ...of Acanthamoeba to harsh conditions such as change in pH, lack of nutrients, treat- ...
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Structural and functional studies of histidine biosynthesis in Acanthamoeba spp. demonstrates a novel molecular arrangement and target for antimicrobials
... that Acanthamoeba is a histidine autotroph with the ability to scavenge this amino acid from the ...of Acanthamoeba and we determine the structure of the complex between Acanthamoeba IGPD and the ...
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Glycogen Phosphorylase in Acanthamoeba spp.: Determining the Role of the Enzyme during the Encystment Process Using RNA Interference
... Cultures of Acanthamoeba. Two Acanthamoeba strains from the American Type Culture Collection, A. castellanii (Neff strain) ATCC 30010, genotype T4, and A. astronyxis ATCC 30137, genotype T7 (5, 47), and two ...
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Histological studies of selected organs of mice experimentally infected with Acanthamoeba spp.
... The present studies provide evidence of the di- versified pathogenic properties of free-living amoe- bae. The most virulent was AD 16, since in the mice infected with this strain all the organs studied were affected. ...
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Genotypic diversity of Acanthamoeba strains isolated from Chilean patients with Acanthamoeba keratitis
... of Acanthamoeba isolates collected at the Parasitology Laboratory of the Public Health Institute of Chile, the only laboratory in the country where Acanthamoeba screening is ...of Acanthamoeba in ...
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A Study of the Lhires III Spectrograph on the Hard Labor Creek Observatory 20 inch Telescope
... Free-living amebae are ubiquitous in the environment and occasionally invade and parasitize host tissues causing illness in humans. 1,2 Although human infections are rare, when illness does occur, it is often fatal. ...
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OLIGODYNAMIC ACTION OF COPPER AND SILVER ON WATERBORNE PATHOGENS
... Mycobacterium, Flavobacterium, Naegleriafowleri, Acanthamoeba spp.) To avoid this contamination, the Municipal Corporation uses many methods such as filtration, settlin[r] ...
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Species Identification of Acanthamoeba Strains Isolated from Patients Referring to Farabi Eye Reference Center Using PCR-RFLP Method
... of Acanthamoeba isolated from infected eyes, such as Acanthamoeba castellani, Acanthamoeba palestinensis, Acanthamoeba griffini, Acanthamoeba culbertsoni, Acanthamoeba polyphaga, ...
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Genotyping of Acanthamoeba Species Isolated from Keratitis Patients by PCR Sequencing Methods in Tehran, Iran
... In this study, all positive samples belonged to T4 genotype the results of which are consistent with the findings of previous studies [11, 26- 28]. T4 has been confirmed as a predominant Acanthamoeba genotype in ...
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Acanthamoeba mediated cytopathic effect correlates with MBP and AhLBP mRNA expression
... In this study, all the Malaysian clinical isolates from corneal scrapings were identified as Rns genotype T4. Currently, there were 35 T4 subgenotypes with deposited GenBank data and published in the articles, which were ...
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Biochemical and molecular characterization of grape rhizospheric microorganisms in Vani and Nandur Naka regions of Maharashtra, India
... The same protocol (above mentioned) was followed for isolation of fungi, instead of media the Potato dextrose Agar for Trichoderma spp and Sabouraud Dextrose Agar for Aspergillus spp was used with 10 day ...
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Algal assay studies of the Chowan River, North Carolina
... OsciZZatoria spp. in N-enriched samples. Other nutrients were not considered.. and Anacystis spp. The diatom Nitzschia spp. A1 though Nitzschia spp.. Numbers of the gree[r] ...
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BACTERIOLOGICAL EXAMINATION OF ABATTOIR WITH REFERENCE TO ESCHERICHIA COLI AND STAPHYLOCOCCUS SPECIES
... Escherichia spp with 39cfu, Staphylococcus spp 36cfu and Bacillus spp ...Staphylococcus spp had the highest average bacteria count, followed by Escherichia coli, Klebsiella spp, ...
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