adeno-associated viral virus
Intracellular Viral Processing, Not Single-Stranded DNA Accumulation, Is Crucial for Recombinant Adeno-Associated Virus Transduction
... Adeno-associated virus (AAV) is a unique gene transfer vector which takes approximately 4 to 6 weeks to reach its expression ...of viral DNA and virions revealed that ss AAV DNA was protected ...
9
The Adeno-Associated Virus Type 2 Regulatory Proteins Rep78 and Rep68 Interact with the Transcriptional Coactivator PC4
... The adeno-associated virus type 2 (AAV-2) Rep78/Rep68 regulatory proteins are pleiotropic effectors of viral and cellular DNA replication, of cellular transformation by viral and ...
10
Mutants at the 2-Fold Interface of Adeno-associated Virus Type 2 (AAV2) Structural Proteins Suggest a Role in Viral Transcription for AAV Capsids
... on viral entry, nuclear entry, uncoating, or second-strand ...the virus capsid normally has a mechanism to bypass the inhibition of the snRNP complex, which is defective in the mu- ...
9
In Vitro Packaging of Adeno-Associated Virus DNA
... Adeno-associated virus (AAV) is a parvovirus and is com- posed of three structural proteins and a linear, single-stranded DNA (ssDNA) genome of approximately ...two viral open reading frames, ...
7
Structural Insights into Adeno-Associated Virus Serotype 5
... The adeno-associated viruses (AAVs) display differential cell binding, transduction, and antigenic characteristics specified by their capsid viral protein (VP) ...are associated with ...
13
Upstream AP1- and CREB-Binding Sites Confer High Basal Activity on the Adeno-Associated Virus Type 5 Capsid Gene Promoter
... during viral infection, and thus capsid gene expression during infection by wild-type viral genomes that contain two ITRs may be ...minute virus of mice and AAV2 are expressed in a temporal manner ...
9
Transcriptional Analysis of the Adeno-Associated Virus Integration Site
... of viral la- ...the viral and cellular promot- ...the viral Rep protein might be able to influence the transcriptional activity of the target locus, which in turn could aid in the maintenance of ...
14
Virus-Mediated Transduction of Murine Retina with Adeno-Associated Virus: Effects of Viral Capsid and Genome Size
... Six AAV serotypes have been described (AAV1 to -6). Their genomes consist of a single strand of DNA approximately 4.7 kb in length, with inverted terminal repeats (ITRs) flanking Rep- and Cap-encoding regions (7, 30). ...
10
Adeno-Associated Virus Type 2 Contains an Integrin α5β1 Binding Domain Essential for Viral Cell Entry
... Plasmids, viral vectors, and biochemical ...(47). Viral genome titers were determined using a dot blot hybridization method with a radiolabeled luciferase transgene probe ...
9
Incorporation of Antigens into Viral Capsids Augments Immunogenicity of Adeno-Associated Virus Vector-Based Vaccines
... Three viral capsid proteins, VP1, VP2, and VP3, build up the nonenveloped AAV capsid ...for viral infectivity ...compromising viral infectivity or affecting viral tropism (10, ...
5
Isolation of the viral DNA replication complex from adeno-associated virus type 1-infected cells.
... The reaction mixture 100 pi contained 50 mM Trishydrochloride, pH 7.9, 2.5 mM MgC12, 2 mM DTT, 444 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The replication compl[r] ...
7
Herpes Simplex Virus Type 1/Adeno-Associated Virus Hybrid Vectors Mediate Site-Specific Integration at the Adeno-Associated Virus Preintegration Site, AAVS1, on Human Chromosome 19
... Helper virus-free HSV-1 amplicons can accommodate for- eign DNA of any size between 1 and 150 kbp (40). As HSV-1 amplicons give room to almost unlimited combinations of ge- netic elements, they belong to the most ...
11
Structure of Adeno-Associated Virus Type 4
... FIG. 2. Parvovirus structures at low resolution. A comparison of surface-shaded low-resolution maps of AAV2 (in red), AAV4 (in blue), AAV5 (in dark green), ADV (in orange), B19 (in pink), CPV (in cyan), DNV (in purple), ...
12
Interaction of the adeno-associated virus Rep protein with a sequence within the A palindrome of the viral terminal repeat.
... Since the pattern of Rep-DNA complexes was not significantly different when the A stem or TR was used as a substrate, it is likely that the 10:1 ratio found for the A stem reflects the s[r] ...
9
Infectious Entry Pathway of Adeno-Associated Virus and Adeno-Associated Virus Vectors
... helper virus of AAV infection, we observed, using double labeling (Cy3Ad and Cy2AAV-3), little if any interac- tion or cocompartmentalization of these particles after the infection process was initiated, whereas ...
9
A cis-Acting Element That Directs Circular Adeno-Associated Virus Replication and Packaging
... AAV lytic replication appears to proceed along two different pathways marked by the assembly of linear or circular duplex intermediates. While the origin of the conventional pathway has been well characterized, ...
11
Adeno associated virus: from defective virus to effective vector
... helper virus such as Ad or her- pes simplex virus (HSV) whereas the latter is established in host cells in the absence of a helper ...the virus genome into a region of roughly 2-kb on the long arm ...
17
Expression Profiles of Bovine Adeno-Associated Virus and Avian Adeno-Associated Virus Display Significant Similarity to That of Adeno-Associated Virus Type 5
... (ORF) in the left-hand end of the genome and a large Cap protein-encoding ORF in the right-hand end, which are sepa- rated by a small intron. Both utilize three similarly placed promoters to express their genomes; ...
12
Cloning and Characterization of Adeno-Associated Virus Type 5
... the viral DNA with T4 DNA polymerase and then digesting it with ...simian virus 40 origin (SV40ori) fragment adjacent to the p5 promoter at the 59 end of the AAV5 RepCap fragment, and 5RepCapB has the ...
11
Adeno-associated virus type 2 DNA replication in vivo: mutation analyses of the D sequence in viral inverted terminal repeats.
... Correlation between rescue and replication functions. Ter- minal resolution is a key step during AAV DNA replication. Several studies have suggested that rescue without replication or replication without rescue can occur ...
6