Agrobacterium-mediated transient expression
AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analyses in Arabidopsis seedlings
... that Agrobacterium-mediated transformation effi- ciency may be compromised when plants recognize Agro- bacterium MAMPs by corresponding pattern-recognition receptors (PRRs) to trigger PTI and block ...
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An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leaves
... the Agrobacterium strain appeared critical be- cause of specific host-bacteria interactions (Tzfira and Citovsky ...In Agrobacterium–mediated in planta transformation, the genotype is generally found ...
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Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay
... protoplast-based transient transformation assay was recently developed to validate the function of pre- dicted enhancers/promoters in maize ...research. Agrobacterium-mediated transient gene ...
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The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species
... Agrobacterium-mediated transient assays harness the natu- ral capability of Agrobacterium to transfer foreign DNA into plant cells with an intact cell wall ...[6] mediated by Agrobacte- ...
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Agrobacterium Mediated Transformation of Maize (Zea mays L.)
... of transient GUS expression in ...the Agrobacterium efficiently infect the tissues by increasing in exposed surface, and stably transformed maize embryogenic tissue ...
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Agrobacterium-mediated transformation systems of Primula vulgaris
... biolistic transient expression experiments, we assessed different 35S-UidA cassettes, and expres- sion of the UidA reporter gene was visualised by a β-glucuronidase (GUS) ...
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A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)
... a transient transformation system for gene characterization in upland ...for Agrobacterium‑mediated transformation assays, we evaluated multiple transforma‑ tion parameters such as ...
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An efficient transient expression system for gene function analysis in rose
... of Agrobacterium into rose petal for silencing specific genes [12, ...leaves, Agrobacterium infiltra- tion is extremely difficult because of the stratum corneum and wax coat present on the outermost layer ...
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Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat
... higher transient expression levels but lower regeneration frequencies [21] than smaller ones ...for Agrobacterium transformation of ...during Agrobacterium co-cul- tivation increased ...
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13. Establishing Agrobacterium mediated transient gene expression assay in Nicotiana tabacum
... transient expression could be thousand times more powerful than in stable ...gene expression studies in plants namely electroporation or, polyethylene glycol (PEG) based transformation of ...
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Transformation Studies in Solanaceous Plants
... Agrobacterium-mediated transient gene expression system in intact plant leaves is a rapid and useful method for analysis of gene ...of Agrobacterium-mediated transformation ...
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Functional Aspects of Silencing and Transient Expression of psbS in Nicotiana benthamiana
... with transient gene expression systems in ...achieve Agrobacterium-mediated transient gene expression in Arabidopsis have been un- successful consistent with a previous report of ...
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A simple and efficient transient transformation for hybrid aspen (Populus tremula × P. tremuloides)
... demonstrated transient gene expression via biolistic bombardment, protoplast transfection, and Agrobacterium co-cultivation ...[12]. Transient transfec- tion of Populus protoplasts isolated ...
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Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID)
... The SPAID removes a central issue in syringe-mediated agroinfiltration—the perpendicular introduction of bac- terial suspensions to the leaf surface. When performed by hand, imperfect seals or uneven pressure on ...
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High-throughput Agrobacterium-mediated barley transformation
... the Agrobacterium-mediated barley transformation system [5], however, our intermediate medium was based on regeneration medium not on the callus induction medium as used by Cho et ...
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PEG mediated and Agrobacterium mediated transformation in the mycopathogen Verticillium fungicola
... the Agrobacterium-mediated ...five Agrobacterium-mediated transformants selected at random ; Lane ...five Agrobacterium-mediated transformants selected at random ; Lane ...
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Influence of Agrobacterium rhizogenes strains on hairy roots induction in Trigonella foenum-graecum L. and secondary metabolites production
... by Agrobacterium rhizogenes are a suitable tissue for the production of secondary metabolites, due to the stability and high production of roots without phytohormone in a short ...of Agrobacterium ...
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Standarizing in Planta Agrobacterium Tumefacience Mediated Genetic Transformation Protocol to Develop New Events by Transforming G. Hirsutum Cotton based on Cry1Ac-Cry1Ec Genes
... Plant transformation mediated by the soil plant pathogen is simple method for plant transformation. There are two tumorgenic species i.e. Agrobacterium rhizogene. gram negative soil bacterium, causes crown ...
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An improved protocol for rapid and efficient agrobacterium mediated transformation of tomato for salt tolerance (solanum lycopersicum l )
... the Agrobacterium cell suspension for 15 min with three intermittent ...the Agrobacterium cells for 45 min, blot dried on tissue paper to remove excess bacteria and plated on co- cultivation media (CCM, ...
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Genome as a Tool of Genetic Engineering: Application in Plant and Plant Derived Medicine
... (GMO), Agrobacterium mediated recombination (T- DNA) and genetic engineering using molecular Biotechnology in plant, medicine and biomedicine have been highlighted from technology based different research ...
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