Agrobacterium Tumefaciens
Characterization of Avirulent TnphoA Mutants in Agrobacterium tumefaciens to Enhance Transformation Efficiency
... in Agrobacterium tumefaciens to identify additional genes critical for ...A. tumefaciens expresses endogenous alkaline phosphatase activity that would com- pletely mask the mutant phenotype ...
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Host-Bacteriophage Interaction in Agrobacterium tumefaciens I. Characterization of Bacteriophage R4 and Physiological Changes in the Infected Host Cell
... Characterization of Bacteriophage R4 and Physiological Changes in the Infected Host Cell Received for publication 14 June 1972 Infection of Agrobacterium tumefaciens B6, a tumor-producin[r] ...
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Membrane and Core Periplasmic Agrobacterium tumefaciens Virulence Type IV Secretion System Components Localize to Multiple Sites around the Bacterial Perimeter during Lateral Attachment to Plant Cells
... ABSTRACT Type IV secretion systems (T4SS) transfer DNA and/or proteins into recipient cells. Here we performed immunofluo- rescence deconvolution microscopy to localize the assembled T4SS by detection of its native ...
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COMPARISON OF TRANSFORMATION EFFICIENCY OF FIVE AGROBACTERIUM TUMEFACIENS STRAINS IN NICOTIANA TABACUM L.
... various Agrobacterium strains have been used to accomplish the ...of Agrobacterium tumefaciens strains GV2260, LBA4404, C58C1, AGL1 and EHA105 harboring the plasmid pBin19 to transform ...different ...
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Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis
... adopting Agrobacterium tumefaciens-mediated transformation (AMT) for insertional mutagenesis; this method has been widely used as a genetic engineering technique for plant cells (Feldmann 1991,; Koncz et ...
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Low Co Cultivation Temperature at 20°C Resulted in the Reproducible Maximum Increase in Both the Fresh Weight Yield and Stable Expression of GUS Activity after Agrobacterium tumefaciens Mediated Transformation of Tobacco Leaf Disks
... The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of ...
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VirD2 of Agrobacterium tumefaciens : functional domains and biotechnological applications
... In this study, novel eff ector proteins for use in plant transformation were created by fusion of several biologically interesting protein domains to the relaxase VirD2 of Agrobacterium tumefaciens. All eff ...
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Monomethylamine as a nitrogen source for a nonmethylotrophic bacterium, Agrobacterium tumefaciens
... Colin Murrell Article Title: Monomethylamine as a Nitrogen Source for a Nonmethylotrophic Bacterium, Agrobacterium tumefaciens Year of publication: 2010 Link to published article: http:/[r] ...
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A Pterin Dependent Signaling Pathway Regulates a Dual Function Diguanylate Cyclase Phosphodiesterase Controlling Surface Attachment in Agrobacterium tumefaciens
... pathogen Agrobacterium tumefaciens, c-di-GMP controls attachment and biofilm formation via regulation of a unipolar polysaccharide (UPP) ...A. tumefaciens are controlled in part by the dual-function ...
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Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33
... [9–14]. Agrobacterium tumefaciens S33, previously isolated from the tobacco rhizosphere, has a strong ability to degrade nicotine via a hybrid of the pyridine and pyrrolidine pathways ...
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Insertional mutagenesis in yeasts using T-DNA from Agrobacterium tumefaciens
... bacterium Agrobacterium tumefaciens, which mediates transfer not only to plants but also to yeasts and fungi, could be exploited to generate collections of yeasts containing insertional mutations if there ...
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Thermostabilization of the uronate dehydrogenase from Agrobacterium tumefaciens by semi-rational design
... from Agrobacterium tumefaciens (UdhAt) features the highest cata- lytic efficiency of all characterized Udhs using glucuronic acid as substrate (829 s − 1 mM − 1 ...
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SUGARCANE GENETIC TRANSFORMATION MEDIATED BY AGROBACTERIUM TUMEFACIENS WITH MANNOSE AS SELECTION MARKER
... Different mannose concentration grades were designed to detect the tolerance of sugarcane varieties ROC22 and GT21 during stages of callus induction, differentiation and rooting. The results showed that the two sugarcane ...
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Loss of PopZAt activity in Agrobacterium tumefaciens by Deletion or Depletion Leads to Multiple Growth Poles, Minicells, and Growth Defects
... Chen L, Wood GE, Almeida NF, Woo L, Chen Y, Paulsen IT, Eisen JA, Karp PD, Bovee D, Chapman P, Clendenning J, Deatherage G, Gillet W, Grant C, Kutyavin T, Levy R, Li MJ, McClelland E, Palmieri A, Raymond C, Rouse G, ...
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Peptidoglycan Synthesis Machinery in Agrobacterium tumefaciens During Unipolar Growth and Cell Division
... Phylogenetic analyses. Phylogenetic trees of the PBP proteins were constructed by first gathering homologs to the E. coli K-12 PBP3 or PBP1b proteins within the desired species using Delta-BLAST (NCBI). Signifi- cant ...
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Fundamental discoveries and simple recombination between circular plasmid DNAs led to widespread use of Agrobacterium tumefaciens as a generalized vector for plant genetic engineering
... The Agrobacterium strain carrying the deletion of the internal T- DNA sequences of the Ti plasmid was named pGV3850 (Zambryski et ...with Agrobacterium carrying the wild-type Ti ...
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Transfection in Agrobacterium tumefaciens
... Transfection frequencies in our study were optimal with a concentration of 22.5 Ag of phenol-extracted LR-4 phage DNA/ml.. Low DNA concentrations 1 and 10 ,ug/ml were not sufficient to p[r] ...
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Host-phage interaction on Agrobacterium tumefaciens. IV. Phage-directed protein synthesis.
... Three classes of protein were detected: early proteins, class I, which include a protein capable of shutting off host protein synthesis; class II, proteins which are detected after 30 mi[r] ...
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Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation
... Our first attempt toward ATM-transformation in T. borchii was based on the pBGgHg binary vector (Grimaldi et al. 2005). Although the T-DNA entered host cells, transformation was transient and the organization of T-DNA ...
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Ψ, a Temperate Phage of Agrobacterium tumefaciens, Is Mutagenic
... The same proportion of lysogenic transconjugants was obtained when we used a recipient strain that was not able to adsorb the phage strain VBC010... 288 EXPERT AND TOURNEUR J.[r] ...
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