Alamar blue Assay
ANTI TUBERCULOSIS ACTIVITY OF MURRYA KOENIGII USING TH MICRO PLATE ALAMAR BLUE ASSAY
... The present study describes the Anti-tuberculosis (anti-TB) activity of oil obtained by hydro-distilation from the leaves of Murraya koengii by Micro-plate Alamar blue assay (MABA). The oil of ...
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Rapid, Low Technology MIC Determination with Clinical Mycobacterium tuberculosis Isolates by Using the Microplate Alamar Blue Assay
... 103 Alamar Blue (Accumed International, Westlake, Ohio) reagent and 10% Tween 80 was added to well ...remained blue, the reagent mixture would be added to another control well and the result would be ...
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Evaluating the anti Mycobacterium tuberculosis activity of Alpinia galanga (L ) Willd axenically under reducing oxygen conditions and in intracellular assays
... There has been no anti-TB drug introduced in the past 30 years and the rapid acquisition of drug resistance to the existing drugs necessitates development of new, ef- fective and affordable anti-TB drugs [24]. ...
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ANTI-TUBERCULAR ACTIVITY OF THE RHIZOME OF CURCUMA PSEUDOMONTANA J. GRAHAM
... Tuberculosis is a highly infectious disease, with approximately one third of the world’s population including 26 % from India estimated to be infected it. However, this problem has become serious as Mycobacterium ...
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A REVIEW ON ANTIPROLIFERATIVE ACTIVITY OF PLANT EXTRACTS AGAINST BREAST CANCER CELL LINES
... 3.3. Assay Reported: The in-vitro tetrazolium and resazurin-based reduction assays employed by authors were MTT (3-(4,5-Dimethylthiazol-2-Yl)- 2,5-Diphenyltetrazolium Bromide); CCK-8 (Cell counting kit-8); MTS ...
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Impact of riboflavin UVA photodynamic inactivation (PDI) (collagen crosslinking technique) on viability, cell cycle phase, apoptosis and proliferation of human corneal endothelial cells
... the Alamar blue assay as follows: HCECs were seeded in 24-well cell culture plates at a concentration of ...riboflavin-UVA-PDI, Alamar blue solution was diluted with culture medium for ...
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Formulation development and evaluation of Rifampicin niosomes for anti-tubercular treatment with isoniazid.
... Abbreviations Abbreviation Expansion MABA Microplate Alamar Blue Assay MAI Mycobacterium avium-intracellulare MDR – TB Multidrug Resistant – Tuberculosis MIC Minimum Inhibitory Concen[r] ...
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PHYTOCHEMICAL, CYTOTOXICITY AND DOCKING STUDIES OF SAMANEA SAMAN
... a greater percentage of methanol is added to purify the ethyl acetate fraction (10%), and a single compound was obtained. The bioactive active compound was found to be Pithecolobine having the spectral data of the IR ...
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Design, Synthesis and Biological Evaluation of Novel Antitubercular Agents by Combining Pyrazoline and Benzoxazole Pharmacophores
... screening assay, espe- cially like low cost, high throughput, and time ...Microplate Alamar Blue Assay (MABA) has emerged as an excellent technology ...screening assay, MABA is ...
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Cytosolic Ca2+ shifts as early markers of cytotoxicity
... In order to see whether Ca 2+ shifts are generally predict- ive of cytotoxicity, we investigated here a wide spectrum of toxins in cell lines from different species origin. The toxicity of arsenic trioxide, hydrogen ...
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DESIGN, SYNTHESIS AND EVALUATION OF ANTITUBERCULAR ACTIVITY OF AMINO AZETIDINONES FROM ISONIAZID
... ABSTRACT: Azetidin-2-one analogues are reported to exhibit various pharmacological activities like cholesterol absorption inhibitory activity, human tryptase, thrombin and chymase inhibitory activity, vasopressin V1a ...
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Evaluation of anticancer compounds from suspension cultures of Holy Basil (Ocimum sanctum L.)
... The assay is based on the fact that the chromophore is negatively charged and does not interact with the cell unless the membrane is ...this assay, the cells are washed with Hank’s buffered salt solution ...
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Synthesis of Novel 2-Butyl-1H-Benzo [4, 5] Imidazo [1, 2-A] Imidazo [4, 5-E] Pyridine-5-Carbonitrile Derivatives and Evaluation of Their Anticancer Activity
... dispensed in a microfuge tube, to the same tube 20 µL of 0.4% trypan blue solution was added. The solution was mixed gently and transferred into a haemocytometer using micropipette where 10µL of cell culture was ...
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<p>Inhibition of EGFR Signaling and Activation of Mitochondrial Apoptosis Contribute to Tanshinone IIA-Mediated Tumor Suppression in Non-Small Cell Lung Cancer Cells</p>
... MTS assay and trypan blue exclusion assay showed that ectopic overexpression of Mcl-1 rescued the reduction of cell viability (Figure 5B), and increased the population of live cells (Figure 5C) in ...
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Ethyl Acetate Extract of Senna alata (L) Roxb Increases Cytotoxicity in the Human Breast, Prostate and Colorectal Cancer Cells
... The clonogenic cell survival assay determines the ability of a cell to proliferate indefinitely, thereby retaining its reproductive ability to form a large colony or a clone. This cell is then said to be ...
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Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array
... Therefore, the red channel gives a code of “0”, whereas the blue and green channels give a code of “1”. Although DC2 does not show a visible color change, computer image analysis finds color changes in the green ...
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Physical Characteristics of Ofloxacin Tablets Sold in Uyo and Quantitative Assay by Novel Ion Pair Complexation Technique
... Quantitative assay was by a spectrophotometric measurement of the ion-pair complex of the drug with bromothymol blue ...spectrophotometric assay method used is a simple, sensitive, economical and ...
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Simple visual assay for determination of Pasteurella haemolytica cytotoxin neutralizing antibody titers in cattle sera
... Cytotoxin neutralization titers obtained by the visual assay were comparable to those determined by the trypan blue exclusion and 51Cr-release methods.. The visual assay was used to meas[r] ...
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The Effect of Growth Differentiation Factor-5, 6, 7 in Chondrogenic Cell Differentiation of ATDC-5
... alcian blue staining, which is indicative of extracellular matrix formation, without affecting aggrecan gene expression in the mouse pluripotent mesenchymal cell line C3H10T1/2 ...
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Evaluation of Polyherbal Anticancer Tablets: A Review
... Caspase-9, alamar blue, LDH assay, XTT assay, sulforhodamine-B assay, MTT assay, DNA fragmentation assay, neutral red uptake cytotoxic assay, tryphan blue ...
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