alanine scanning
Alanine-Scanning Mutagenesis of Protein Phosphatase Type 1 in the Yeast Saccharomyces cerevisiae
... If most of the defects caused by the alanine-scanning alleles result from im- paired interactions between the mutant products and Glc7p binding proteins, then the fact[r] ...
12
Alanine Scanning of Poliovirus 2CATPase Reveals New Genetic Evidence that Capsid Protein/2CATPase Interactions Are Essential for Morphogenesis
... RNA transcription, transfection, and in vitro translation. Wt and mutant plasmids of pT7PVM were linearized at a unique EcoRI restric- tion site and used as templates for in vitro RNA synthesis using T7 RNA polymerase. ...
12
Alanine Scanning of the Hepatitis C Virus Core Protein Reveals Numerous Residues Essential for Production of Infectious Virus
... the alanine substitutions in this region did not affect the efficiency of SPP ...with alanine, rather than with the bulkier leucine or valine, did not disrupt SPP ...to alanine led to the production ...
12
Charged-to-Alanine Scanning Mutagenesis of the N-Terminal Half of Adeno-Associated Virus Type 2 Rep78 Protein
... The adeno-associated virus (AAV) Rep78 and Rep68 proteins are required for site-specific integration of the AAV genome into the AAVS1 locus (19q13.3-qter) as well as for viral DNA replication. Rep78 and Rep68 bind to the ...
12
Identification and characterization of a temperature-sensitive mutant of human immunodeficiency virus type 1 by alanine scanning mutagenesis of the integrase gene.
... charge-cluster-to-alanine scanning mutagenesis (3, 4, 10, 12, 25). It substitutes alanine residues for any two charged amino acids within a 5-amino-acid scan ...
5
Structural and Functional Analysis of Interhelical Interactions in the Human Immunodeficiency Virus Type 1 gp41 Envelope Glycoprotein by Alanine-Scanning Mutagenesis
... used alanine-scanning mutagenesis to investigate the importance of individual side chains in determining the biophysical properties of the gp41 core and the membrane fusion activity of the gp120-gp41 ...
11
Charge Cluster-to-Alanine Scanning of UL128 for Fine Tuning of the Endothelial Cell Tropism of Human Cytomegalovirus
... Markerless mutagenesis of HCMV genomes. The highly endotheliotropic HCMV TB40/E-derived BAC TB40-BAC4 (26) was used for the generation of mutant strains. The mutant BACs were generated with the markerless mutagen- esis ...
8
On the effect of mutations in bovine or camel chymosin on the thermodynamics of binding κ-caseins
... computational alanine scanning, we show that similar results are obtained using the PSE-3, PC and PC+ functionals because the differences between these functionals partially cancel ...
36
Directional Transneuronal Infection by Pseudorabies Virus Is Dependent on an Acidic Internalization Motif in the Us9 Cytoplasmic Tail
... the alanine scanning Us9 proteins not only aided in the visualization of the various proteins in transfected cells but was also necessary for subsequent endocytosis assays (see ...Us9–EGFP alanine ...
13
Genetic Analysis of the Carboxy-Terminal Region of the Hepatitis C Virus Core Protein
... Comprehensive alanine-scanning mutagenesis of core amino acids 170 to 191 was performed to identify residues that play important roles in the C-terminal processing of the protein and/or in the production of ...
8
Structure/Function Analysis of the Saccharomyces cerevisiae Trf4/Pol σ DNA Polymerase
... “surface-targeted” alanine scanning mutations in TRF4 have identified those regions required for Pol ’s essential function, for its role in DNA double-strand break repair, and for its association with ...
11
Determinants in the Maturation of Rubella Virus P200 Replicase Polyprotein Precursor
... eral alanine-scanning mutations in the N-terminal alpha helix, and packaged replicon assays showed that rescue could be medi- ated by CP in the virus ...
13
Fine Mapping of the Interaction of Neutralizing and Nonneutralizing Monoclonal Antibodies with the CD4 Binding Site of Human Immunodeficiency Virus Type 1 gp120
... Alanine scanning mutagenesis was performed on monomeric gp120 of human immunodeficiency virus type 1 to systematically identify residues important for gp120 recognition by neutralizing and nonneutralizing ...
17
Cross-Reactive HIV-1-Neutralizing Human Monoclonal Antibodies Identified from a Patient with 2F5-Like Antibodies
... CA). Alanine-scanning mutant peptides for surface plasmon resonance (SPR) analysis were based on a wild-type MPER peptide encompassing gp41 residues 657 to 670 followed by a C9 tag ...
9
Role of the Transmembrane Domains of prM and E Proteins in the Formation of Yellow Fever Virus Envelope
... We investigated here the role of the TM domains of prM and E in the formation of the envelope of the flavivirus yellow fever virus (YFV). We used alanine scanning insertion mu- tagenesis (4, 25) in these ...
8
The Autographa californica Multicapsid Nucleopolyhedrovirus GP64 Protein: Analysis of Transmembrane Domain Length and Sequence Requirements
... generated alanine substi- tution mutations in single or multiple positions to examine the potential function of the glycine or phenylalanine residues in GP64 function ...the alanine scanning ...
15
Bromodomain Protein 4 Mediates the Papillomavirus E2 Transcriptional Activation Function
... of alanine-scanning substitution mutants of the human papillomavirus type 16 E2 N-terminal transactivation domain, we found that amino acids required for Brd4 binding were also required for transcriptional ...
10
N Acetyl L alanine
... acid alanine has, during the last 15 years, been developed to be the standard material for radiation dosimetry using electron paramagnetic resonance (EPR) spectrometry as the readout technique (Regulla & ...
6
L Alanine hydrochloride monohydrate
... The distances and angles of the present L-alanine molecule are consistent in the typical values reported in the literature of L-alanine molecules (See Table 1 and Figure 1). The atoms N, C2, C3, O1, and O2 ...
6
N Acryloyl L alanine
... substitution reaction of acryloyl chloride with l -alanine. In the crystal structure, intermolecular N—H O and O—H O hydrogen bonds link the molecules into a three-dimensional network, in which they may be ...
5