Alexa Fluor 488
Inflammatory pre-conditioning restricts the seeded induction of α-synuclein pathology in wild type mice
... e – f , Co-immunofluorescence staining with GFAP (astrocyte marker, Alexa Fluor 594) or NeuN (neuronal marker, Alexa Fluor 594) with 81A antibody (Alexa Fluor 488) shows α Syn inclusions[r] ...
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Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging
... AF488: Alexa Fluor 488; ATCC: American type culture collection; BSA: bovine serum albumin; CDI: N,N'-carbonyldiimidazole; DAPI: 4',6-diamidino-2- phenylindole; DLS: dynamic light scattering; DMEM/ ...
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Endosomal Localization of the Serum Resistance-Associated Protein in African Trypanosomes Confers Human Infectivity
... Human infectious T. brucei rhodesiense is able to resist TLF-1 killing through the expression of the VSG-like protein SRA. The molecular basis of resistance remains unclear. How- ever, it is evident that the ...
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Altered expression of two zinc transporters, SLC30A5 and SLC30A6, underlies a mammary gland disorder of reduced zinc secretion into milk
... antibody Alexa Fluor 488 anti-mouse (Chemicon, Melbourne, Australia) diluted at 1:2000 was added and samples were incubated for 2 h at room temperature following by PBS ...
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Preparation of stable tau oligomers for cellular and biochemical studies
... VQIXXK 311 and 337 VEVKSE 342 ) and non-covalent bonding [22–25]. Although either mechanism would in principle be sufficient, aggregation efficiency is increased in the presence of both [21]. In this study, it was ...
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Changes in calcitonin gene-related peptide (CGRP) receptor component and nitric oxide receptor (sGC) immunoreactivity in rat trigeminal ganglion following glyceroltrinitrate pretreatment
... The sources, characteristics, and dilutions of the primary and secondary antibodies used in this study are listed in Tables 1 and 2. After rinsing in phosphate-buffered saline (PBS; 0.01 M, pH 7.4), the mounted sections ...
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A rapid sensitive, flow cytometry based method for the detection of Plasmodium vivax infected blood cells
... (MRA28) Alexa Fluor 660 at a 1:100 di- lution alone and in combination with anti-DBP (3D10) Alexa Fluor 488 at 1:100 dilution or with anti-PvMSP1- 19 Alexa Fluor555 at 1:100 ...
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Protocol: an improved and universal procedure for whole-mount immunolocalization in plants
... plus Alexa Fluor ® 488 goat anti-mouse IgG as secondary antibody diluted 1:800 (shown in green color) and H + -ATPase (AS07 260) rabbit primary antibody plus Alexa Fluor ® 555 goat ...
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Development of Norwalk Virus-Specific Monoclonal Antibodies with Therapeutic Potential for the Treatment of Norwalk Virus Gastroenteritis
... of Alexa Fluor 488, which is directed to the c-myc tag at the C terminus of VP1 (display fluorescence), and DyLight 649, which is directed to the MAb/Fabs (Ab binding ...
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The effect of blood coagulation factor XIII on fibrin clot structure and fibrinolysis
... Human fibrinogen, human glu-plasminogen, horse radish peroxidase conjugated (HRP) antibodies to factor XIII-A (SAF-13 HRP) and 2 -antiplasmin (A2AP HRP) were purchased from Enzyme Research Laboratories (Swansea, UK). ...
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α Synuclein propagates from mouse brain to grafted dopaminergic neurons and seeds aggregation in cultured human cells
... A 3 hours after addition of 1 μM of Alexa Fluor 488–labeled α-syn proteins in the culture medium, HEK cells expressing α-syn–DsRed have taken up tagged α-syn monomers left, oligomers mid[r] ...
12
Tunable Plasmonic Nanoprobes for Theranostics of Prostate Cancer
... Microscopy images of living cells: bright field (A,C,E,G), time-resolved optical scattering (F,H) and fluorescent (Alexa Fluor 488 conjugated to PSMA antibody, B,D) images of prostate c[r] ...
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The Human Papillomavirus Type 16 E5 Oncoprotein Inhibits Epidermal Growth Factor Trafficking Independently of Endosome Acidification
... Immunofluorescence microscopy. Procedures for the transfection, paraformal- dehyde fixation, saponin permeabilization, and immunolabeling of COS cells grown on 22-by-22-mm glass coverslips have been described in detail ...
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Inhibition of Middle East Respiratory Syndrome Coronavirus Infection by Anti-CD26 Monoclonal Antibody
... of Alexa Fluor 488-labeled ADA or ADA2 (CECR1) as a fluorescence ...with Alexa Fluor 488-labeled ADA in the presence of various concentrations of the anti-CD26 MAb 2F9 (dark ...
8
Increased frequency of circulating IL 21 producing Th cells in patients with granulomatosis with polyangiitis (GPA)
... Abbreviations ANCA: anti-neutrophil cytoplasmic autoantibodies; A488: Alexa Fluor®® 488; APC: allophycocyanin; BAFF: B-cell activating factor; BVAS: Birmingham Vasculitis Activity Score;[r] ...
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Anti-c-Met antibodies recognising a temperature sensitive epitope, inhibit cell growth
... Binding of monoclonal antibody to native c-Met expressed on the cell surface of live SNU-5 cells was detected using anti-mouse secondary antibody conjugated with Alexa Fluor®488 dye and [r] ...
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Improving vascular maturation using noncoding RNAs increases antitumor effect of chemotherapy
... or Alexa 488–conjugated siRNA packaged into CH/Endo28-NPs and tumors were harvested 6 hours after NP ...with Alexa 488–con- jugated siRNA were noted in more than 70% of the fields and examined ...
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Antibodies to the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in cerebellar ataxia
... (Euroimmun), Alexa Fluor® (AF) 488 (Invitrogen, Karlsruhe, Germany) or AF568 (Invitrogen), polyclonal donkey anti-human IgG antibodies labeled with Rhodamin Red-X (Dianova, Hamburg, Germany), and ...
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The Human Polyomavirus, JCV, Uses Serotonin.pdf
... receptors but do not express 5HT 2A or 5HT 2c serotonergic receptors. HeLa cells were incubated with irrelevant antibody (solid histograms), with monoclonal anti- bodies to the 5HT 2A R and 5HT 2C R (top panels, open ...
5
PubMedCentral-PMC4852977.pdf
... S3), Alexa Fluor 680 conjugated BSA loaded particles (Table S1) were intra-articularly delivered to the knee joint of ...the Alexa Fluor 680 conjugated BSA particles in the joint 12 hr post ...
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