Alfalfa mosaic virus
mRNAs containing the unstructured 5' leader sequence of alfalfa mosaic virus RNA 4 translate inefficiently in lysates from poliovirus-infected HeLa cells.
... capped alfalfa mosaic virus coat protein mRNA (AlMV CP RNA), which contains an unstructured 5 leader sequence, is unusual in being functionally active in extracts prepared from poliovirus-infected ...
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Coat Protein Activation of Alfalfa Mosaic Virus Replication Is Concentration Dependent
... Alfalfa mosaic virus (AMV) and ilarvirus RNAs are infectious only in the presence of the viral coat protein; therefore, an understanding of coat protein’s function is important for defining viral ...
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Functional equivalence of common and unique sequences in the 3' untranslated regions of alfalfa mosaic virus RNAs 1, 2, and 3.
... of alfalfa mosaic virus (AMV) RNAs 1, 2, and 3 consist of a common 3 * -terminal sequence of 145 nucleotides (nt) and upstream sequences of 18 to 34 nt that are unique for each ...
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Alfalfa Mosaic Virus Coat Protein Bridges Rna and Rna-dependent Rna Polymerase in Vitro
... Available evidence suggests that replication of alfalfa mosaic virus (AMV) and ilarvirus RNAs is initiated de novo at the RNA 3' termini. AMV and ilarvirus RNAs lack the canonical tRNA 3'-terminal ...
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In Vitro Transcription by the Turnip Yellow Mosaic Virus RNA Polymerase: a Comparison with the Alfalfa Mosaic Virus and Brome Mosaic Virus Replicases
... yellow mosaic virus RNA to initiate minus-strand synthesis in vitro is the 3 ⴕ ACCA ...brome mosaic virus (BMV) and alfalfa mosaic virus ...
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Mutations in coat protein binding sites of alfalfa mosaic virus RNA 3 affect subgenomic RNA 4 accumulation and encapsidation of viral RNAs.
... of alfalfa mosaic virus (AMV) contain a specific binding site for coat protein (CP) and act as a promoter for minus-strand RNA synthesis by the purified AMV RNA-dependent RNA polymerase (RdRp) in an ...
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Evaluation of the Conformational Switch Model for Alfalfa Mosaic Virus RNA Replication
... Key elements of the conformational switch model describing regulation of alfalfa mosaic virus (AMV) replication (R. C. Olsthoorn, S. Mertens, F. T. Brederode, and J. F. Bol, EMBO J. 18:4856–4864, ...
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The structure of alfalfa mosaic virus capsid protein assembled as a T=1 icosahedral particle at 4.0-A resolution.
... K. Fukuyama, S. S. Abdel-Meguid, J. E. Johnson, and M. G. Rossmann (J. Mol. Biol. 167:873–984, 1983) reported the structure of alfalfa mosaic virus assembled from the capsid protein as a T 5 1 ...
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In vitro genetic selection analysis of alfalfa mosaic virus coat protein binding to 3'-terminal AUGC repeats in the viral RNAs.
... RNA-protein interactions in alfalfa mosaic virus (AMV) and the related ilarviruses are essential for virion assembly and stability (18, 19). In addition, a defining characteristic of AMV and the ...
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Viral Coat Protein Peptides with Limited Sequence Homology Bind Similar Domains of Alfalfa Mosaic Virus and Tobacco Streak Virus RNAs
... of alfalfa mosaic virus (AMV) and the ilarviruses (type member, tobacco streak virus) bind to the 39 termini of the genomic viral RNAs (23, 28, ...of virus replication (9, ...initiating ...
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Alfalfa Mosaic Virus Replicase Proteins P1 and P2 Interact and Colocalize at the Vacuolar Membrane
... The alphavirus-like superfamily comprises a large number of animal and plant viruses sharing the properties of a capped, positive-stranded RNA genome and homologies in their RNA replication proteins. Despite homologies ...
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Role of the Alfalfa Mosaic Virus Methyltransferase-Like Domain in Negative-Strand RNA Synthesis
... Alfalfa mosaic virus (AMV) is a positive-strand RNA virus belonging to the family Bromoviridae. It has a tripartite ge- nome, with RNAs 1 and 2 encoding the replicase proteins P1 and P2, ...
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Role of the 3′-Untranslated Regions of Alfalfa Mosaic Virus RNAs in the Formation of a Transiently Expressed Replicase in Plants and in the Assembly of Virions
... Alfalfa mosaic virus (AMV) RNAs 1 and 2 encode the replicase proteins P1 and P2, respectively, whereas RNA 3 encodes the movement protein and the coat protein ...
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Accumulation of alfalfa mosaic virus RNAs 1 and 2 requires the encoded proteins in cis.
... The genome of alfalfa mosaic virus (AlMV) consists of three plus-strand RNAs. RNAs 1 and 2 encode the replicase proteins P1 and P2, respectively, whereas RNA 3 encodes the putative viral movement ...
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Role of an Essential Triloop Hairpin and Flanking Structures in the 3′ Untranslated Region of Alfalfa Mosaic Virus RNA in In Vitro Transcription
... of Alfalfa mosaic virus (AMV), a tripartite plant virus belonging to the family Bromoviridae, is located within the 3 ⴕ -terminal 145 nucleotides (nt), which can adopt a tRNA-like structure ...
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In Vitro and In Vivo Studies of the RNA Conformational Switch in Alfalfa Mosaic Virus
... The role of AMV CP has been the subject of extensive research in the past four decades. Initially, it was found that, in contrast to RNAs of the Bromo-, Cucumo-, and Oleavirus gen- era, the genomic RNAs of AMV and the ...
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A new mechanism to confer resistance to Alfalfa mosaic virus infection in white clover and tobacco using mutations to the ATP binding motif
... It would seem that the low level of gene expression as observed in the northern blots from the transgenic plants might be sufficient to confer resistance to virus infection and to attenu[r] ...
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Susceptibility of ten red clover (Trifolium pratense) cultivars to six viruses after artificial inoculation
... (4n). alfalfa mosaic virus (AMV, family: Bromoviridae, genus: alfamovirus), Clover yellow mosaic virus (ClYMV, alphaflexiviridae, Potexvirus), Clover yel- low vein virus (ClYVV, ...
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Aspects of the molecular biology of potato virus Y
... Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic plants.. Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus c[r] ...
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Panicum Mosaic Virus and Its Satellites Acquire RNA Modifications Associated with Host Mediated Antiviral Degradation
... Currently, it remains unclear whether the modified viral RNAs enhance or attenuate PMV pathogenicity. Paradoxically, PMV is frequently associated with both SPMV and the satellite RNAs (25–28, 30), which have opposing ...
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