Allele specific effects on expression
Allele Specific Effects on Extracellular Superoxide Dismutase Synthesis and Secretion
... sponsible for observed differences in the ecSOD phenotype in mice. Moreover, one of the amino acids substitutions (N21D) is positioned at the site of signal peptide cleavage and may thus produce changes in processing ...
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Advances in the identification and analysis of allele-specific expression
... Although numerous methods have been developed to assay ASE, they usually require RNA to be available and are dependent upon genetic polymorphisms (such as single nucleotide poly- morphisms (SNPs)) to differentiate ...
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Loss of RNA expression and allele-specific expression associated with congenital heart disease
... gene expression was observed only in CHD subjects (9/37 CHD subjects; 0/57 GTEx subjects; P ¼ ...downregulated expression could phenocopy genes harbouring loss-of-function (LOF) mutations in one ...
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High-throughput allele-specific expression across 250 environmental conditions
... genotypic effects across individuals that have different genetic backgrounds, allele-specific expression (ASE) approaches compare allelic effects within individuals, thereby controlling ...
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Novel Method for Analysis of Allele Specific Expression in Triploid Oryzias latipes Reveals Consistent Pattern of Allele Exclusion
... Assessing allele-specific gene expression (ASE) on a large scale continues to be a technically challenging ...the expression of a whole set of alleles. Other more subtle effects on ASE ...
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Data analysis issues for allele-specific expression using Illumina's GoldenGate assay
... Recent examinations of two-color data from Illumina ’s Infinium platform have revealed that normalization can reduce dye-bias [19]. In this paper, we focus on the data analysis issues that arise when Illumina GoldenGate ...
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SCALE: modeling allele specific gene expression by single cell RNA sequencing
... overall expression levels between the two ...their effects cancel out when looking at the mean ...more specific model SCALE has higher power to detect it compared to an analysis based on mean allelic ...
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Profiling allele-specific gene expression in brains from individuals with autism spectrum disorder reveals preferential minor allele usage.
... gene expression 19 , varied patterns are observed across genes in the region impacted by dup15q due to complex local regulatory mechanisms 4,19–21 ...monoallelic expression (MAE) is random MAE (RMAE), which ...
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Regulatory Divergence in Drosophila melanogaster and D. simulans, a Genomewide Analysis of Allele-Specific Expression
... trans effects, which are dependent on experimental design and there are also differences between designs in the interpretation of cis/ trans ...trans effects on expression level can be ...of ...
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Allele-specific expression analysis does not support sex chromosome inactivation on the chicken Z chromosome
... assess allele-specific expression across a far larger number of Z-linked loci than previously assayed (Zimmer et ...compared expression of these genes with the parental expression ...
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Expression Changes Confirm Genomic Variants Predicted to Result in Allele-Specific, Alternative mRNA Splicing
... the effects of such variants has been shown to be accurate using information theory-based ...gene expression microarrays and custom designed q-RT-PCR or TaqMan ...gene expression microarray and ...
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Allele-specific expression of mutated in colorectal cancer (MCC) gene and alternative susceptibility to colorectal cancer in schizophrenia
... downstream effects on the rate of translation, folding and post-translational modifications of nascent polypeptides 24,25 ...gene expression, the differences in the gene’s expression caused by ASE ...
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Engineering heat tolerance in potato by temperature dependent expression of a specific allele of HEAT SHOCK COGNATE 70
... A2 allele To account for the different expression characteristics of the A2 allele of HSc70, the promoter sequences of the four different alleles in the parents of the 06H1 population were ...
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Validating discovered Cis-acting regulatory genetic variants: application of an allele specific expression approach to HapMap populations.
... An important feature of the ASE approach is its potential to detect cis-acting effects, as this approach is robust to environmental confounders and trans-acting effects. It is possible to map cis-acting ...
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Visualizing Allele Specific Expression In Single Cells
... With this approach, the SNP oligonucleotides will not cross-hybridize, giving us the ability to discriminate between a single base pair di↵erence, but there remains another specificity challenge. Since they are comprised ...
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Global Analysis of Allele-Specific Expression in Arabidopsis thaliana
... Gene expression is a complex trait determined by various genetic and nongenetic ...with allele-specific expression, and 1688 genes controlled by composite trans-regulatory ...of ...
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ALLELE-SPECIFIC MODIFICATION OF THE EXPRESSION OF THE Adh2 GENE IN MAIZE BY ETHYLENE
... Because of the low activity of the ADH-2 enzyme and the poor resolution of the ADH-2.ADH-2 dimer bands i n electrophoretograms, the relative expressions of the Ad[r] ...
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Allele-specific transcriptional elongation regulates monoallelic expression of the IGF2BP1 gene
... to allele-speci- fic expression, it should be detectable through allele-spe- cific association of CTCF and ...monoallelic expression in a pedigree of LCLs correlates with mono- allelic binding ...
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MBASED: allele specific expression detection in cancer tissues and cell lines
... an allele with a larger read count at each SNV to the ‘major’ haplotype, with the im- plicit assumption that ASE is consistent in one direction along the length of the ...major allele (haplotype) frequency ...
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Identifying Potential cis-Regulatory Variants Associated with Allele-Specific Expression
... Eukaryotic cellular programs are context dependent. Furthermore, gene regulation exerts this control through layers of interacting molecules. Therefore, genetic variation could interfere in the way these molecules behave ...
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