Amplification and quantitation using real time PCR:
Real-time PCR for mrna quantitation
... a real-time PCR experiment, from experimental planning and setup through data analysis and graphical presentation ...errors using two different mathematical models, both correcting for ...
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Comparison of real time PCR and hemagglutination assay for quantitation of human polyomavirus JC
... that real-time PCR is a sensitive and reliable method for in vitro quantitation of ...and real-time PCR was more reliable than the HA assay for in vitro calculation of ...
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Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR
... TaqMan Real time PCR reaction Real time PCR reactions were performed using Roche Lightcycler TaqMan master ...performed using a Roche Lightcycler 480 thermal cycler ...
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Comparison and Evaluation of Real Time PCR, Real Time Nucleic Acid Sequence Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae
... duplex real-time PCR assay for detection of ...control real-time PCR is described. In addition, real-time nucleic acid sequence-based amplification (NASBA) ...
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The sensitivity of real time PCR amplification targeting invasive Salmonellaserovars in biological specimens
... PCR amplification, both in the presence or absence of antimicrobials (Table ...However, PCR is not commonly reported for the routine identification of invasive Salmonellae , this is in spite of a ...
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Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites
... RNA amplification methods for detection of Leishmania parasites have advantages regarding sensitivity and potential quantitative characteristics in comparison with conventional diagnostic methods but are often ...
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Calibrated Real Time PCR Assay for Quantitation of Human Herpesvirus 8 DNA in Biological Fluids
... several real-time PCR methods are available for quantification of HHV-8 DNA (17, 42, 43, 47), but none allows controlling both the efficiency of the extraction procedure and the presence of ...
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Comparison of a loop mediated isothermal amplification for orf virus withquantitative real time PCR
... isothermal amplification (LAMP) assay based on the identification of the F1L gene was developed for the specific detection of ORFV ...of real-time ...and real-time PCR assays ...
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Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and. murine cytomegalovirus internal control
... novel real-time PCR assay described in this paper offers several important advantages for HBV DNA ...on’ time due to robotic nucleic acid extraction and real-time amplicon ...
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Comparison of Different Methods of Isolation of DNA of Commonly Encountered Candida Species and Its Quantitation by Using a Real Time PCR Based Assay
... genomic amplification methods such as real-time PCR assay has been reported as an alternative to conventional culture for early detection of invasive ...that PCR detection of free ...
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Development of real time PCR for detection and quantitation of Dengue Viruses
... Results: The 3'UTR of thirteen Indian strains of DENV was sequenced and aligned with 41 representative sequences from GenBank. A region conserved in all four serotypes was used to target primers and probes for the ...
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A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... A real-time PCR assay for ...search using the Pk probe sequence did not identify any potential cross-reactivity with other patho- gens or human ...
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Event-specific method for the quantitation of maize line DAS using real-time PCR. Validation Report
... For relative quantification of event 59122 DNA, a maize-specific reference system amplifies a 79-bp fragment of Hmg (High mobility group), a maize endogenous gene, using a pair of Hmg gene-specific primers and an ...
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Identification and Quantitation of Cashmere (Pashmina) Fiber and Wool Using Novel Microchip Based Real Time PCR Technology
... the PCR reagent mix. It is sub- stantially faster than traditional real-time PCR systems for being carried as miniature reaction volume in metal ...
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Real time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
... This can be proved by the values of variation co- efficients of intra- and inter-assays and by linear course of both parallel standard curves BVDV/RNA and BVDV/cDNA. High specificity of the reac- tion was confirmed by ...
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Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
... Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected ...copies ...
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Simultaneous Detection, Subgrouping, and Quantitation of Respiratory Syncytial Virus A and B by Real Time PCR
... Clinical Virology Department, Applied Immunology and Microbiology Division, Wyeth Vaccines, Pearl River, New York 1 ; Wellesley College, Wellesley, Massachusetts 2 ; and Department of Microbiology, Chinese University of ...
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Development of a Rapid Real Time PCR Assay for Quantitation of Pneumocystis carinii f sp carinii
... QTD PCR assay is able to detect fewer than 5 copies of the rat-derived ...specimen. Using cloned templates ...compared quantitation by light microscopy with that by QTD PCR but found that ...
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Comparison between Two Amplification Sets for Molecular Diagnosis of Toxoplasmosis by Real Time PCR
... patients. PCR has clearly improved the diagnosis of toxoplas- mosis and is today an inescapable technique for revealing the presence of the parasite in clinical ...
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Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR
... labor, time and the amount of reagents are signif- icantly reduced using minimum mixtures, we compared the results from nested qPCR mixtures prepared with 1/ 50 volume samples from preamplified mixtures ...
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