Amplification technique
Establishment of the 1st World Health Organization International Standard for Plasmodium falciparum DNA for nucleic acid amplification technique (NAT) based assays
... Malaria diagnosis has relied upon microscopic analysis of Wright's or Wright-Giemsa stained blood smears to detect infection and determine parasite loads [7]. Slide interpre- tation requires considerable expertise and is ...
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Adaptation of a visualized loop mediated isothermal amplification technique for field detection of Plasmodium vivax infection
... A visualized closed-tube LAMP method was established utilising microcrystalline wax-dye capsules, which can deliver highly sensitive DNA fluorescence dye to the reaction mixture following amplification by melting ...
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Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis
... To confirm the fidelity of amplification, some LAMP positive products were cross-validated by sequencing [29] with modifications. For this, 1 μl of the LAMP product was included in a PCR with two outer primers, F3 ...
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A Multicenter Study Evaluation of the Digene Hybrid Capture II Signal Amplification Technique for Detection of Hepatitis B Virus DNA in Serum Samples and Testing of EUROHEP Standards
... The lack of standardization has been a problem for nucleic acid detection assays, although in theory, molecular biology- based assays are ideal for standardization purposes (3, 6). With standard hybridization-based ...
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Three Isothermal Amplification Techniques for Rapid Identification of Cladophialophora carrionii, an Agent of Human Chromoblastomycosis
... isothermal amplification of DNA (LAMP), a recently developed detection technique (12), may offer a cheaper alternative without compromising sensitivity and ...autocycling amplification of DNA using ...
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Biomolecules Detection Using a Silver Enhanced Gold Nanoparticle Based Biochip
... silver amplification technique; (b) characterization of the extent to which the nanoparticle adsorption can be quantified using silver enhancement; (c) characterization of two distinct biomolecular ...
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Results: Smear positive cases CBNAAT positivity was 95.91% and smear negative cases 10.48%.
... In the era of rapid diagnosis of TB and Drug resistance Cartridge Based Nucleic Acid Amplification Technique (CBNAAT) came with a future of early and easy diagnosis for TB. However Microscopic examination ...
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Clinical Profile and outcome of Tuberculosis in Patients with Diabetes
... 2 ATT and 1 patient was put on CAT 4 ATT. Out of 61 patients put on CAT I, 53 patients got cured (88%), 2 patients failed treatment (3%), 6 patient defaulted treatment (9%). Those who failed and defaulted treatment were ...
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Trichomoniasis and associated co-infections of the genital tract among pregnant women presenting at two hospitals in Ghana
... Acid Amplification Technique; pH: Power of hydrogen; PID: Pelvic Inflammatory Disease; RTIs: Reproductive Tract Infections; SBAHS: School of Biomedical and Allied Health Sciences; ...
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Diagnosing Herpesvirus Infections by Real Time Amplification and Rapid Culture
... real-time technique were developed to demonstrate the presence of herpes simplex virus type 1 (HSV-1) and HSV-2, varicella zoster virus (VZV), and cytomegalovirus (CMV) in miscellaneous clinical ...The ...
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Human immunodeficiency virus 1 infection of macrophages in vitro neither induces tumor necrosis factor (TNF)/cachectin gene expression nor alters TNF/cachectin induction by lipopolysaccharide
... The synthesis of tumor necrosis factor (TNF)/cachectin was assessed in primary monocyte- derived macrophage (MDM) cultures after in vitro infection with a macrophage-tropic strain of HIV-1 (HTLV-IIIBa-L/85). Productive ...
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Development and evaluation of a Loop Mediated Isothermal Amplification (LAMP) technique for the detection of hookworm (Necator americanus) infection in fecal samples
... isothermal amplification technique (LAMP) is a technique developed by Notomi et ...acid amplification technique uses only one enzyme ( Bst DNA polymerase) and is able to amplify large ...
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Progression Toward an Improved DNA Amplification Based Typing Technique in the Study of Mycobacterium tuberculosis Epidemiology
... VNTR technique within a population (n ⫽ 125) where the majority of isolates present were shown by RFLP, spoligotyp- ing, and available contact tracing data to be ...VNTR technique was performed blinded to ...
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Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients
... extraction technique for the extraction of Mu DNA in clinical samples using a modified GenoLyse ® DNA extraction ...extraction technique with our already developed RPA assay for the effective diagnosis of ...
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Isothermal Amplification and Molecular Typing of the Obligate Intracellular Pathogen Mycobacterium leprae Isolated from Tissues of Unknown Origins
... serial amplification of nucleic acids with whole- genome ...PCR-based amplification, several methods have been developed, including balanced PCR ...isothermal amplification has provided a means to ...
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Designing, Optimization and Validation of Tetra Primer ARMS PCR Protocol for Genotyping Single Nucleotide Polymorphism rs4731702 (C/T) of KLF14 Gene Associated with Type 2 Diabetes Mellitus: A Study in San Luis, Argentina
... morphism (PCR-RFLP), Single Strand Conformation Polymorphism (SSCP), direct DNA sequencing, and tetra-primer Amplification Refractory Mutation System-Poly- merase Chain Reaction (ARMS-PCR) for genotyping of ...
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Rapid identification of low level hepatitis B related viral genome in serum
... the amplification of viral DNA by the polymerase chain reaction ...The amplification is initiated using "generic" primers derived from the core and ...
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Biomarker development in MET-targeted therapy
... gene amplification rates were detected depending on the detection method ...high amplification. In gastric cancer, MET gene amplification prevalence varies from 2 to 23% among studies limited by ...
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Evaluation of amplification refractory mutation system (ARMS) technique for quick and accurate prenatal gene diagnosis of <em>CHM</em> variant in choroideremia
... for amplification refractory mutation system (ARMS) were designed and PCR conditions were ...ARMS technique to identify if they inherited the pathogenic gene from parental generation; we used amniotic fluid ...
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Cross-amplification and characterization of microsatellite markers in Alcantarea patriae Versieux & Wand.
... The individuals were collected in the district of Vila Cruzeiro, in the municipality of Jerônimo Monteiro. Twelve markers were polymorphic and 10 monomorphic, with an amplification success rate of 71%. The ...
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