APC/C
APC/C-Cdh1-dependent anaphase and telophase progression during mitotic slippage
... from APC/C-Cdc20. It allows active APC/C- Cdc20 to drive cells into anaphase by inducing the degradation of securin and cyclin ...mitotic APC/C sub- strates, including securin, ...
15
Visualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C).
... the APC/C, thus competing effectively for binding sites on the ...lated APC/C at prometaphase, but ubiquitination of cyclin B1 is blocked by the ...better APC/C substrate in ...
27
Glucose and Ras Activity Influence the Ubiquitin Ligases APC/C and SCF in Saccharomyces cerevisiae
... the APC/C are suppressed by reduced Ras signaling activity, by a deletion of the RAS2 gene, by a cdc25 mutation, by elevated levels of PDE2, or by growth without ...
13
Identification of the APC/C co-factor FZR1 as a novel therapeutic target for multiple myeloma
... Multiple Myeloma (MM) is a haematological neoplasm characterised by the clonal proliferation of malignant plasma cells in the bone marrow. The success of proteasome inhibitors in the treatment of MM has highlighted the ...
13
The Mitotic Checkpoint Complex Requires an Evolutionary Conserved Cassette to Bind and Inhibit Active APC/C.
... apo-APC/C. APC/C immunoprecipitated from Cdc20-depleted mitotic HeLa cell extract was pre-incubated on ice with rMCC and recombinant ...37 C for 15 ...
11
SUMO targets the APC/C to regulate transition from metaphase to anaphase
... endogenous APC/C purified from HeLa cells. First, the purified APC/C was SUMOylated in ...endogenous APC/C with the SUMOylation machinery lacking the SUMO conjugating enzyme ...the ...
15
The APC/C activator FZR1 is essential for meiotic prophase I in mice
... cells we performed immunohistochemistry on D18 testis. In agreement with previous reports we observed strong immunoreactivity to cyclin B1 in the cytoplasm of spermatocytes from control mice (Fig. 6A) (Brandeis et al., ...
12
Suppression of APC/C Cdh1 has subtype specific biological effects in acute myeloid leukemia
... Cdh1 is an important regulator of proliferation and differentiation. While we found Cdh1 expression decreased in the vast majority of primary AMLs of all subtypes, Cdh1 deficiency contributes to the differentiation block ...
11
lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2
... yeast APC/C revealed two subcomplexes that are held together by the largest subunit, Apc1 ...other APC/C subcomplex contains Apc2, Apc10/Doc1 and Apc11 subunits, and connects to Apc1 through ...
17
The APC/C activator FZR1 coordinates the timing of meiotic resumption during prophase I arrest in mammalian oocytes
... the APC/C, which is an E3 ligase that regulates mitotic and meiotic progression through the ubiquitylation and subsequent degradation of key sets of substrates (Peters, ...the APC/C by ...
9
How eggs arrest at metaphase II: MPF stabilisation plus APC/C inhibition equals Cytostatic Factor
... the c-Mos/MEK/MAPK pathway in the metaphase arrest of the two most widely studied vertebrates, frog and ...to c-Mos ...specific APC/C inhibitor whose degradation is Ca 2+ dependent has greatly ...
7
Studies on the Contribution of Human Cytomegalovirus UL21a and UL97 to Viral Growth and Inactivation of the Anaphase-Promoting Complex/Cyclosome (APC/C) E3 Ubiquitin Ligase Reveal a Unique Cellular Mechanism for Downmodulation of the APC/C Subunits APC1, APC4, and APC5
... to APC/C function both in the absence of other viral proteins and in the context of infection with a mutant lacking both genes, we used a Cre recombination-based system to conditionally express UL21a and ...
12
Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor.
... Apc11 (modelled on Cul4a-Rbx1 of SCF) and Cdc27 (ref. 26 ). Only the N-terminal β-strand of Apc11 bound to the Apc2 CTD is modelled (orange). The two subunits of Cdc27 are shown in light and dark green. View in (a) shows ...
14
Quantitative framework for ordered degradation of APC/C substrates
... core APC/C subunits [15, 16]. Complete APC/C Cdc20 activation also requires inactivation of the spindle assembly checkpoint (SAC), which monitors proper attachment of sister chromatids to the ...
18
Running on a treadmill: dynamic inhibition of APC/C by the spindle checkpoint
... The steady state levels of MCC are determined by the rates of MCC formation and disassembly. Three possible sce- narios can explain the higher concentrations of MCC in mitosis. First, the rate of MCC formation increases ...
6
Insights into APC/C: from cellular function to diseases and therapeutics
... which APC/C recognizes, and catalyzes ubiquitination of the tar- geting proteins [13, ...the APC/C complex consists of three sub-complexes: the scaffolding subcom- plex platform, the catalytic ...
18
APC/C – the master controller of origin licensing?
... Loss of APC/C regulation has been implicated in cancers. The Evi5 chromosomal locus is frequently targeted for recombination in several cancers and a truncated Evi5 is expressed in a patient with ...
6
Meiotic control of the APC/C: similarities & differences from mitosis
... by APC/C Ama1 as cell exit meiosis II ...of APC/C dependent and inde- pendent mechanisms to accomplish this ...of APC/C Ama1 is mitigated prior to anaphase I, consistent with a ...
7
Delayed APC/C activation extends the first mitosis of mouse embryos.
... whether Emi2 was responsible for the delay in cyclin degradation and the prolonged zygotic 167.. M-phase, we depleted Emi2 with specific morpholinos (Emi2 MO), which had been shown to [r] ...
27
The Anaphase-Promoting Complex/Cyclosome Is Required for Anaphase Progression in Multinucleated Ashbya gossypii Cells
... between APC/C core subunits, are conserved compared to those in the budding yeast homologue ...have C boxes (conserved in Cdc20 family members), which are regions thought to link these tar- geting ...
16