B. abortus
Immune Responses and Protection against Experimental Brucella suis Biovar 1 Challenge in Nonvaccinated or B. abortus Strain RB51-Vaccinated Cattle
... In conclusion, our study suggests that RB51 vaccination of cattle will not protect against infection after exposure to B. suis bv. 1. Although it is unlikely that economic losses due to abortion will occur, cattle ...
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Determination of stability of Brucella abortus RB51 by use of genomic fingerprint, oxidative metabolism, and colonial morphology and differentiation of strain RB51 from B abortus isolates from bison and elk
... Brucella abortus RB51 and isolates from cattle, bison, and elk were characterized by pulsed-field gel electrophoresis and standard techniques for biotyping Brucella species, which included biochemical, morpho- ...
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Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9.
... a B. abortus-soluble fraction was also used as a solid-phase antigen in ELISA tests (11, 24), it contained LPS, which may lead to the undesired reactivities mentioned ...
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Brucella abortus conjugated with a gp120 or V3 loop peptide derived from human immunodeficiency virus (HIV) type 1 induces neutralizing anti-HIV antibodies, and the V3-B. abortus conjugate is effective even after CD4+ T-cell depletion.
... when B. abortus or KLH was used as carriers suggests that these carriers stim- ulate different cytokines in the immunized mice, probably as a consequence of stimulating different subsets of Th ...
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Influenza viral vectors expressing the Brucella OMP16 or L7/L12 proteins as vaccines against B abortus infection
... immune responses to both (L7/L12 or Omp16) protein of B. abortus . The optimal T-cell immune responses were achieved by the c. immunization route, despite the fact that influenza viruses primarily reproduce ...
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Isolation and molecular detection of brucella melitensis from disease outbreak in sheep and b abortus from cattle farm by is711 and omp2a gene based pcr
... 2006). B. melitensis, B. suis and B. abortus are pathogenic for man and are listed as potential bio-weapons by the Centers for Disease Control and Prevention in the ...are B. melitensis ...
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DETECTION OF B ABORTUS S19 ON UTERINE RAT (RATTUS NORVEGICUS) WITH IMMUNOHISTOCHEMICAL METHOD
... Results: B. abortus S19 cell to the rat show the presence of inflammatory cells and bleeding in the uterine ...B. abortus S19. Rabbit antibody to 36 kDa OMP can detect B. abortus ...
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Cattle serologically positive for Brucella abortus have antibodies to B. abortus Cu-Zn superoxide dismutase.
... Lane 1, amido black-stained blot of BCSP; lane 2, immunoblot incubated with positive control serum from an experimen- tally infected cow (cow 47); lane 3, immunoblot with a negative cont[r] ...
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Sero Prevalence of Brucellosis among Nomadic Herdsmen, Abattoir and Livestock Workers in Niger Delta Region, Nigeria
... Brucella is gram-negative bacteria that are pathogenic to humans and a variety of livestock animals and wildlife. The genus Brucella has six recognized species on the basis of host specificity. While all six species ...
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THE CHARACTERIZATION OF GENE ENCODING SURFACE PROTEIN-31(BCSP-31) Brucella abortus FIELD ISOLATE BY PCR TECHNIQUE
... cells B. abortus S19 are being used in the serological test and making it is quite difficult to distinguish the antigen reactivity with antibodies from the vaccine and ...
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Optimization of Brucella abortus Protocols for Downstream Molecular Applications
... ABSTRACT We compared the performances of various DNA extraction kits for their ability to recover Brucella abortus strain 19 inoculated into Brucella-free bovine tis- sues. Tissues were homogenized in a FastPrep ...
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Brucellosis Vaccines: An Overview
... 25 k dalton outer membrane protein, which is introduced for usage in this kind of vaccine (72). DNAK as a cytoplasmic protein and SurA as a Periplasmic peptidyl prolyl cis -trans isomerase have been also evaluated in ...
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Characterization and Protective Property of Brucella abortus cydC and looP Mutants
... Brucella abortus readily multiplies in professional or nonprofessional phagocytes in vitro and is highly virulent in ...of B. abortus biovar 1 strain IVKB9007 lacking the ATP/GDP-binding protein ...
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Assessment of Fluorescence Polarization Titres as a Prediction Indicator of Which Brucella Spp. Circulate in a Domestic Ruminant Animal Populations
... Brucellosis is a bacterial zoonotic disease that affects domestic and wild animals, including humans [4, 10, 11, 12]. The disease is caused by various bacteria in the family Brucella, which tend to infect specific animal ...
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VirB12 Is a Serological Marker of Brucella Infection in Experimental and Natural Hosts
... The Brucella species type IV secretion system, encoded by the virB1-12 locus, is required for intracellular replication and persistent infection in vivo. The requirement of VirB proteins for infection suggests that they ...
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Recent advances in Brucella abortus vaccines
... of B. abortus vaccines ...virulent B. abortus strain at the most susceptible period (mid-gestation), and the protection is measure by the ability of the vaccine in preventing abor- tion ...
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A 6 Nucleotide Regulatory Motif within the AbcR Small RNAs of Brucella abortus Mediates Host Pathogen Interactions
... in B. abortus, but, importantly, the data suggested that a nonlethal C-terminal truncation of RNase E could be constructed in ...B. abortus (Xavier DeBolle, personal ...in B. ...
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Characterization of Brucella abortus mutant strain Δ22915, a potential vaccine candidate
... Brucellosis, caused by Brucella spp., is an important zoonosis worldwide. Vaccination is an effective strategy for protec- tion against Brucella infection in livestock in developing countries and in wildlife in developed ...
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Protection Provided by an Encapsulated Live Attenuated ΔabcBA Strain of Brucella ovis against Experimental Challenge in a Murine Model
... S19 vjbR mutant encapsulated with alginate conferred greater pro- tection than nonencapsulated B. abortus S19 in mice (33). Simi- larly, a live attenuated B. melitensis vjbR mutant strain provided ...
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EVALUATION OF IMMUNE RESPONSE AGAINST REDUCED DOSE OF Brucella abortus STRAIN 19 VACCINE ADMINISTERED THROUGH CONJUNCTIVAL ROUTE IN CATTLE
... Whole blood samples were collected from jugular vein of animals using 18 gauge needle attached to a vacutainer coated with heparin (Beckton Dickinson, Cat. No. 367874). on 0, 21 st , 60 th , 90 th , 120 th , 150 th and ...
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