B. burgdorferi
Three species of Borrelia burgdorferi sensu lato (B burgdorferi sensu stricto, B afzelii, and B garinii) identified from cerebrospinal fluid isolates by pulsed field gel electrophoresis and PCR
... Borrelia burgdorferi sensu lato cerebrospinal fluid isolates (mainly from southern Germany) were analyzed by pulsed-field gel electrophoresis (PFGE) for large restriction fragment pattern (LRFP) and linear plasmid ...
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Innate Immune Responses to Infection by the Pathogenic Spirochetes Leptospira interrogans and Borrelia burgdoferi.
... Myd88 3/3 !BMDMs.!BMDMs(were(stimulated( with(media,( B.*burgdorferi* (Bb)(at(an(MOI(=(10,(RNA(from( B.*burgdorferi* (Bb(RNA)(at( 1ug,(or(the(TLR7(agonist(Imiquimod((R848)(at(1ug(for(24([r] ...
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Infection Prevalence of Borrelia burgdorferi in Ticks Collected from Songbirds in Far Western Canada
... Borrelia burgdorferi, the Lyme disease ...with B. burgdorferi. Transstadial transmission of B. burgdorferi occurred from larva to nymph, plus nymph to adult, in the avian coastal tick, ...
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Recombinant BBK32 Protein in Serodiagnosis of Early and Late Lyme Borreliosis
... Borrelia burgdorferi, which is char- acterized by multistage skin, joint, neurologic, and cardiac manifestations ...of B. burgdorferi sensu lato as causes of human LB: ...B. burgdorferi ...
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Scored Antibody Reactivity Determined by Immunoblotting Shows an Association between Clinical Manifestations and Presence of Borrelia burgdorferi sensu stricto, B garinii, B afzelii, and B Valaisianain Humans
... of B. burgdorferi sensu lato obtained in North America and Europe has demonstrated that OspA has antigenic variability and that several distinct groups can be serologically defined (45, ...
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Phenotypic analysis of outer surface protein C (OspC) of Borrelia burgdorferi sensu lato by monoclonal antibodies: relationship to genospecies and OspA serotype
... that B-cell epitopes recognized by humans and mice might be ...OspC-specific B-cell epitopes recognized by ...of B. burgdorferi sensu ...
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Possible role of glial cells in the onset and progression of Lyme neuroborreliosis
... CSF samples were maintained on ice after collection until processed. Total cell numbers were determined using a Reichert Bright-Line metalized hemocytometer (Thermo Fisher Scientific, Waltham, MA) within 30 min of ...
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Molecular typing of Borrelia burgdorferi sensu lato by PCR restriction fragment length polymorphism analysis
... from B. burgdorferi sensu stricto, and additional studies will be neces- sary to ascertain whether atypical isolates are truly unique or only appear that way because of limited population ...for B. ...
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Improved immunoglobulin M serodiagnosis in Lyme borreliosis by using a mu capture enzyme linked immunosorbent assay with biotinylated Borrelia burgdorferi flagella
... A ,u-capture enzyme-linked immunosorbent assay ELISA for detection of serum immunoglobulin M IgM antibodies to Borrelia burgdorferi by using biotinylated purified B.. burgdorferi flagell[r] ...
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Graebsch, Almut (2010): Crystal structure, nucleic-acid binding properties, and dimerization model of Pur-alpha. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... The crystallized PUR domain (D. melanogaster Pur-α repeats I-II) was found to be a functional nucleic-acid domain by EMSAs and filter binding assays (section 2.2.1.3). Assessment of the degree of conservation and the ...
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Michel, Holger (2005): Entwicklung und Evaluierung molekularbiologioscher Nachweismethoden zur Spezies- und OspA-Typ-Differenzierung von Borrelia burgdorferi sensu lato. Dissertation, LMU München: Medizinische Fakultät
... jedoch eine ausgeprägte Heterogenität (79,6-100 % Überseinstimmung), wobei sich, entsprechend den OspA-Typen von B. garinii [215], homogene Gruppen bilden lassen [204]. Ausgangspunkt bei der Entwicklung der ...
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Direct Molecular Typing of Borrelia burgdorferi Sensu Lato Species in Synovial Samples from Patients with Lyme Arthritis
... Borrelia burgdorferi, have been isolated from synovial ...of B. burgdorferi sensu lato, which is able to differentiate seven different Borrelia ...samples: B. burgdorferi sensu stricto ...
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Genetic Diversity of Borrelia burgdorferi and Detection of B bissettii Like DNA in Serum of North Coastal California Residents
... Borrelia burgdorferi sensu lato ...americana, B. andersonii, B. bissettii, B. burgdorferi sensu stricto, ...only B. burgdorferi sensu stricto (hereinafter referred to as ...
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Species Specific Serodiagnosis of Lyme Arthritis and Neuroborreliosis Due to Borrelia burgdorferi Sensu Stricto, B afzelii, and B garinii by Using Decorin Binding Protein A
... Culturing B. burgdorferi from clinical samples other than erythema migrans lesions is difficult (43), and the PCR-based methods seem to be too insensitive for routine laboratory testing for LB (29, 37), ...
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Lyme disease and current aspects of immunization
... complete avoidance of wooded areas, simple physical measures such as wearing long sleeves and long pants that are tucked into the socks, and drastic measures such as area application of insecticides [82]. Tick and insect ...
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Analysis and comparison of plasmid profiles of Borrelia burgdorferi sensu lato strains
... of B. burgdorferi sensu stricto was statisti- cally significantly smaller (50 kb) than the equivalent plasmids (55 to 56 kb) of ...and B. afzelii. We also found that large plasmids of B. ...
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Serodiagnosis of Lyme borreliosis by Borrelia burgdorferi sensu stricto, B garinii, and B afzelii western blots (immunoblots)
... the performance and clinical utility of B. burgdorferi serological assays. Magnarelli et al. (21) concluded that the use of antigens from local strains was not critical to their enzyme immunoas- say’s ...
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Anaplasma phagocytophilum-Borrelia burgdorferi Coinfection Enhances Chemokine, Cytokine, and Matrix Metalloprotease Expression by Human Brain Microvascular Endothelial Cells
... phils are markedly activated for the production of chemokines (IL-8) and cytokines (IL-6) (15, 26), biologically active com- pounds with multiple effects, including enhanced changes in vascular permeability related to ...
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Identification of Ehrlichia spp andBorrelia burgdorferi in Ixodes Ticks in the Baltic Regions of Russia
... at 99°C, and cooled rapidly on ice. The slots of the miniblotter were filled with the denatured PCR product, and hybridization was performed for 1 h at 42°C. The membrane was removed from the miniblotter and washed twice ...
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When a patient suspected with juvenile idiopathic arthritis turns out to be diagnosed with an infectious disease – a review of Lyme arthritis in children
... The B. burgdorferi genome has a unique ...disseminated B. burgdorferi infection, whereas RST2 and RST3 strains are less frequently detected ...
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