bacterial artificial chromosomes
Oncogenicity of Virulent Marek's Disease Virus Cloned as Bacterial Artificial Chromosomes
... Bacterial artificial chromosomes (BAC) containing full- length genomes of several herpesviruses have enabled the ap- plication of rapid mutagenesis strategies to identify functions of individual ...
5
An easy and versatile 2 step protocol for targeted modification and subcloning of DNA from bacterial artificial chromosomes using non commercial plasmids
... Bacterial artificial chromosomes (BACs) are low copy plasmids based on the F-plasmid of E. coli. They can carry up to 300 kb of foreign DNA. The large insert size has made BACs an important source ...
6
Combined use of bacterial artificial chromosomes-on-beads with karyotype detection improves prenatal diagnosis
... the bacterial artificial chromosomes (BACs)-on-Beads (BoBs ™ ) technology for the detection of common aneu- ploidies and specific microdeletion syndromes [1, 9 – ...
8
High-Resolution Pachytene Chromosome Mapping of Bacterial Artificial Chromosomes Anchored by Genetic Markers Reveals the Centromere Location and the Distribution of Genetic Recombination Along Chromosome 10 of Rice
... Large-scale physical mapping has been a major challenge for plant geneticists due to the lack of techniques that are widely affordable and can be applied to different species. Here we present a physical map of rice ...
9
Genetic Linkage Maps of the Red Flour Beetle, Tribolium castaneum, Based on Bacterial Artificial Chromosomes and Expressed Sequence Tags
... Significance of the Tribolium map: An important ap- plication of the genetic map described here will be its integration with the emerging physical map and ge- nome sequence. The physical map will be based on comparison ...
8
A Molecular Cytogenetic Map of Sorghum Chromosome 1: Fluorescence in Situ Hybridization Analysis With Mapped Bacterial Artificial Chromosomes
... to chromosomes and markers to chromosome arms, by localizing the positions of centromeres on linkage groups, by resolving the order of closely linked markers (and associated BAC clones), and by confirming the ...
10
Sequence Conservation of Homeologous Bacterial Artificial Chromosomes and Transcription of Homeologous Genes in Soybean (Glycine max L. Merr.)
... While there may be a bias toward the types of genes retained in the homeologous BACs analyzed, these findings show that retention of duplicate genes in homeologous regions in soybean may be common. Paleopolyploids are ...
12
The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
... using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene ...
18
Capture Hi C identifies a novel causal gene, IL20RA, in the pan autoimmune genetic susceptibility region 6q23
... of bacterial artificial chromosomes (BACs) (Life Technolo- gies) spanning the region of interest with minimal overlap (Additional file 1: Table ...
15
Cloning of Bovine herpesvirus type 1 and type 5 as infectious bacterial artifical chromosomes
... as bacterial artificial chromosomes ...of bacterial genetics [8], allowing facili- tated generation of recombinant viruses in future ...
6
Loss of SETDB1 decompacts the inactive X chromosome in part through reactivation of an enhancer in the IL1RAPL1 gene
... BAC: bacterial artificial chromosomes; ChIP-Seq: chromatin immunoprecipita- tion coupled with high-throughput sequencing; DHS: DNase I hypersensi- tive site; EZH2: enhancer of Zeste 2; FISH: ...
20
Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red®/ET® Recombination
... The general mechanism of rpsL counter-selection is illus- trated in Fig. 1. A prerequisite for the strain to be used is a chromosomal encoded resistance to streptomycin con- ferred by a mutation in rpsL, which implies ...
8
The development and characterisation of a bacterial artificial chromosome library for Fragaria vesca
... terial artificial chromosome (BAC) libraries have become the vector of choice for the development of DNA libraries in many plant species, including Malus, Prunus and Rosa in the Rosaceae ...
5
Annotated bacterial chromosomes from frame-shift-corrected long-read metagenomic data
... Second-generation sequencing has been the work-horse of metagenomic analysis of microbiomes, with typical studies based on hundreds of millions of short reads [1, 2]. While the taxonomic and functional binning of short ...
13
Comparison of Plant and Microbial Communities between an Artificial Restoration and a Natural Restoration Topsoil in Coal Mining Subsidence Area
... The bacterial community diversity and plant species in the artificial restoration area were higher. than those in the natural area[r] ...
38
A yeast artificial chromosome clone map of the Drosophila genome.
... We describe the mapping of 979 randomly selected large yeast artificial chromosome (YAC) clones of Drosophila DNA by in situ hybridization to polytene chromosomes.. Eight h[r] ...
15
The Impact of Organic Acids and pH on the Virulence Factor Expression of E. coli O157:H7.
... accounting for an estimated 20,000 infections each year (Boyce et al., 1995). Three major virulence genes in E. coli O157:H7 purportedly contribute to its ability to cause disease: genes encoding Shiga toxin (Stx), ...
138
THE CHROMOSOME OF BACILLUS SUBTILIS. II: A GENERAL SOLUTION FOR THE AGE DISTRIBUTION FUNCTION OF BACTERIAL CHROMOSOMES
... When the origin of replication and the order of replication is fixed, as in the bacterial chromosome (YOSHIKAWA and SUEOKA 1963; CAIRNS 1963), the fre- quency g ( X ) of [r] ...
10
12_lecture_cell_cycle.ppt
... Intact nuclear envelope Chromosomes Microtubules Intact nuclear envelope Kinetochore microtubule Kinetochore microtubule Fragments of nuclear envelope Bacterial chromosome. (c) Diatoms a[r] ...
97
A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host
... It can be seen from this simple linear analysis that the stability of S 1 and S 2 depends only upon the bacterial reproductive ratios ρ c and ρ R (of B c and R respectively) and the plant host’s carrying capacity ...
17