Baculovirus expression
Designing a recombinant Bacmid construct of HCV core+1 in Baculovirus expression system
... Background and Objectives: Hepatitis C virus (HCV) chronically infects around 200 million people worldwide and fre- quently causes liver cirrhosis and hepatocellular carcinoma. Rapid detection of this virus results in ...
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Expression of the Herpes Simplex Virus Type 2 Glycoprotein D in Baculovirus Expression System and Evaluation of Its Immunogenicity in Guinea Pigs
... eukaryotic expression systems have been used for in vitro production of the subunit vaccines from foreign ...Eukaryotic expression system is the first choice for production of proteins that undergo ...
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The effect of time and optimization of the baculovirus expression system for more efficient recombinant protein production of porcine epidemic diarrhea virus in insect cells
... The baculovirus expression vector system (BEVS) has many strengths, such as the capacity for large inserts of DNA and a high yield of recombinant ...titer baculovirus stock; (ii) determination of the ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... the Baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) downstream of the strong polyhedrin ...cells. Expression of VP4 in the Sf9 cells was confirmed by the immunofluorescence test using ...
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Expression and characterization of hepatitis B virus surface antigen polypeptides in insect cells with a baculovirus expression system.
... The expression of two S polypeptides by recombinant viruses 373S and 106FS suggested that the polypeptides represented the unglycosylated p25 and glycosylated gp29 forms of the small HBs[r] ...
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Expression of Sindbis virus 26S cDNA in Spodoptera frugiperda (Sf9) cells, using a baculovirus expression vector.
... By immunoblot analysis with antisera directed against whole Sindbis virus and the individual structural proteins capsid, E2, and El, we have shown that polypeptides similar in size and a[r] ...
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Synthesis of functional human immunodeficiency virus tat protein in baculovirus as determined by a cell-cell fusion assay.
... Abundant synthesis of functional human T-cell leukemia virus type I p4Y protein in eucaryotic cells by using a baculovirus expression vector.. A baculovirus vector can express intron-con[r] ...
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Expression, processing, and assembly of foot-and-mouth disease virus capsid structures in heterologous systems: induction of a neutralizing antibody response in guinea pigs.
... Synthesis of foot-and-mouth disease virus capsid proteins in insect cells using baculovirus expression vectors.. T7 RNA polymerase can direct expression of influenza virus cap-binding pr[r] ...
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Optimization of recombinant human transferrin expression in insect cells baculovirus system
... The baculovirus expression vector system (BEVS) is frequently a method of choice for the expression of recombinant mammalian proteins (O’Reilly et ...
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Recombinant virus vaccine for bluetongue disease in sheep.
... Bluetongue virus tubules made in insect cells by recombinant baculovirus: expression of NS1 gene of bluetongue virus serotype 10.. Structure of the bluetongue virus capsid.[r] ...
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Structural Requirements of Astrovirus Virus-Like Particles Assembled in Insect Cells
... The baculovirus expression system has extensively been used to express capsid protein virus genes and to produce self- assembled VLPs which represent a good tool for the study of viral morphogenesis (7, 11, ...
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Serodiagnosis of Mice Minute Virus and Mouse Parvovirus Infections in Mice by Enzyme-Linked Immunosorbent Assay with Baculovirus-Expressed Recombinant VP2 Proteins
... In this report, we describe the development of ELISAs that detect anti-MMV or anti-MPV antibodies in the sera of par- vovirus-infected mice. The MMV ELISA and the MPV ELISA displayed high sensitivities and high ...
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Yellow Fever Virus Replicons as an Expression System for Hepatitis C Virus Structural Proteins
... by expression of the HCV structural pro- teins in the baculovirus expression system (3) or by Semliki Forest virus replicons ...the baculovirus and Semliki Forest virus expres- sion systems ...
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Identification and characterisation of the interaction between Wiskott-Aldrich syndrome protein (WASP) and c-Src kinases
... I initially was unable to co-immunoprecipitate endogenous WASP from cell extracts, with any of its potential binding partners. A variety of cell lines, subjected to different stimuli were used, together with various ...
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A Cellular Drosophila melanogaster Protein with Similarity to Baculovirus F Envelope Fusion Proteins
... like baculovirus F proteins, Dm-cF and the proteins encoded by the f genes from ...the baculovirus F proteins from group I NPVs and GVs (those considered “fusion competent”) (22, 39, 42, 48, 49), the F ...
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Molecular Cloning of a Babesia caballi Gene Encoding the 134-Kilodalton Protein and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay
... antigen could clearly distinguish the B. caballi-infected horse sera from noninfected or B. equi-infected horse sera (10). However, in order to cover all sera infected with various types of field strains, it is necessary ...
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Identification and expression of acid beta glucosidase mutations causing severe type 1 and neurologic type 2 Gaucher disease in non Jewish patients
... ologous expression in Sf9 cells using the baculovirus system revealed that the missense mutations, R48W and V352L, had 14 and 7%, respectively, of the specific activity based on cross-reacting immunologic ...
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Autographa californica Nucleopolyhedrovirus orf69 Encodes an RNA Cap (Nucleoside-2′-O)-Methyltransferase
... vivo expression of ...the expression pattern of MTase1, polyclonal antibodies were first prepared by immuni- zation of rabbits with MTase1 purified from ...maximal expression was reached at 36 h ...
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Formation of Wild-Type and Chimeric Influenza Virus-Like Particles following Simultaneous Expression of Only Four Structural Proteins
... quadruple baculovirus recombinant that simultaneously expresses in Sf9 cells the hemagglutinin (HA), neur- aminidase (NA), matrix (M1), and M2 proteins of influenza virus A/Udorn/72 ...gene baculovirus ...
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Factors regulating baculovirus late and very late gene expression in transient-expression assays.
... late expression factor genes (lefs) are unknown, although two of them, lef-8 and lef-9, encode polypeptides with sequence motifs conserved in many RNA polymerases (27, ...gene expression because it ...
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