Baculovirus expression vector system
The effect of time and optimization of the baculovirus expression system for more efficient recombinant protein production of porcine epidemic diarrhea virus in insect cells
... The baculovirus expression vector system (BEVS) has many strengths, such as the capacity for large inserts of DNA and a high yield of recombinant ...titer baculovirus stock; (ii) ...
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Process development for pandemic influenza VLP vaccine production using a baculovirus expression system
... ent expression systems, including bacterial, yeast, insect, mammalian, and plant systems ...the baculovirus expression vector system (BEVS) [11], which provides a rapid and efficient ...
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Optimization of recombinant human transferrin expression in insect cells baculovirus system
... The baculovirus expression vector system (BEVS) is frequently a method of choice for the expression of recombinant mammalian proteins (O’Reilly et ...
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Expression and purification of biologically active v-sis/platelet-derived growth factor B protein by using a baculovirus vector system.
... The level of v-sis protein expressed in zinc-induced MMT-v-sis cells is the highest we have observed in mammalian cell transfectants when using a variety of promoters, including simian v[r] ...
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Optimization of canine interleukin 12 production using a baculovirus insect cell expression system
... To determine the secretion efficiency and integrity of the recombinant canine IL-12 produced by High-five cells infected with each of the four recombinant bacu- lovirus constructs at the best conditions, Western blot ...
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Evaluation of an Enzyme Linked Immunosorbent Assay with Recombinant Rhoptry Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle
... coli expression vector pGEMEX-2 (Promega ...The vector was designated pGEMEX/RAP-1 and was used to express the RAP-1 polypeptide as a fusion protein with the bacterio- phage T7 gene 10 leader peptide ...
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Expression of Sendai virus nucleocapsid protein in a baculovirus expression system and application to diagnostic assays for Sendai virus infection
... Construction of recombinant virus. Standard DNA manipulation techniques were used to construct recombinant plasmids (9). The amplified PCR product was cleaved with BamHI (Gibco BRL), resolved in a 1% agarose gel, and ...
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Expression of Aleutian mink disease parvovirus proteins in a baculovirus vector system.
... Coomassie blue-stained SDS-10% polyacrylamide gel A and immunoblot B analysis of total lysates of Sf9 cells infected with three different recombinant AcNPVs expressing the structural pro[r] ...
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Locust ion transport peptide (ITP): primary structure, cDNA and expression in a baculovirus system
... was cloned into the baculovirus transfer vector pVL1393 (Webb and Summers, 1990) and recombinant plasmids were selected as described previously and sequenced. Fresh cultures of Spodoptera frugiperda (Sf9) ...
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Designing a recombinant Bacmid construct of HCV core+1 in Baculovirus expression system
... Five μl of 10X PCR reaction buffer, 1 μl dNTP mix and 0.5 μl of PFU DNA polymerase (Fermentas Life Sceince, Lithuania), and 40.5 μl dDW. This reac- tion was amplified in a Master cycler Gradient (Ep- pendorf, Germany) ...
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Expression and purification of envelop capsid proteins of foot and mouth disease virus type o isolated in vietnamin baculovirus system for making virus like particle
... Dual vector which contains two multiplecloning sites ...entire expression cassettes between Tn7R and Tn7L were transferred from pFast-VP0-VP1-2A- VP3 to the Bacmid by site-specific transposition with the ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... Primer design. Oligonucleotide primers specific for genome segment no. 4, were designed according to rotavirus SA11 sequence data (accession number: D16345) [21]. To generate att (attachment sites) flanked VP4 PCR ...
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A baculovirus dual expression system based vaccine confers complete protection against lethal challenge with H9N2 avian influenza virus in mice
... The baculovirus vector contains a large gen- ome that enables insertion of large, foreign DNA frag- ments or the construction of multivalent ...this system using appropriate selection of HA genes ...
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Regulation of porcine circovirus type 2-like particles expressed in baculovirus expression system
... protein expression by two- to 20-fold and lower both the RNA level and the rate of transcription initiation from the promoter (Ooi et ...an expression vector surrounded by a reporter gene, the ...
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Self-Assembly of Epstein-Barr Virus Capsids
... the baculovirus expression vector (BEV) system was used to express the capsid shell proteins BcLF1 (major capsid protein), BORF1 (triplex protein), BDLF1 (triplex protein), and BFRF3 (small ...
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... of therapeutic protein in target cells [41]. Thus, improving the transfection efficiency. Finally finding appropriate animal models will help the scientific faculty to carry the research in right direction without ...
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Characterization of baculovirus-expressed herpes simplex virus type 1 glycoprotein K.
... We report here for the first time that i HSV-1 gK was expressed in a baculovirus expression system; ii the expressed gK was detected in a partially glycosylated precursor pgK form and a [r] ...
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Synthesis of viruslike particles by expression of the putative capsid protein of Leishmania RNA virus in a recombinant baculovirus expression system.
... Western blot analysis B and Coomassie blue staining A of proteins from lysates of 3 x 106 SF9 cells in 100-mm tissue culture dishes mock-infected lanes 2 or infected with wild-type AcMNP[r] ...
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Regulation of expression of a baculovirus ecdysteroid UDPglucosyltransferase gene.
... Characterization of an early gene accelerating expression of late genes of the baculovirus Autographa californica nuclear polyhedrosis virus.. Basic methods in molecular biology.[r] ...
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Structural Requirements of Astrovirus Virus-Like Particles Assembled in Insect Cells
... The baculovirus expression system has extensively been used to express capsid protein virus genes and to produce self- assembled VLPs which represent a good tool for the study of viral morphogenesis ...
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