Baculovirus vector
N-acetyl-beta-glucosaminidase accounts for differences in glycosylation of influenza virus hemagglutinin expressed in insect cells from a baculovirus vector.
... The hemagglutinin of fowl plague virus has been expressed in Spodoptera frugiperda (Sf9) cells and in Estigmene acrea cells by using a baculovirus vector. Structural analysis revealed that the ...
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Construction of a Baculovirus vector containing A subunit of Shiga toxin for protein delivery.
... recombinant baculovirus vector was designed carrying gene encoding A subunit of ...in baculovirus. Presumably, baculovirus expressions are along with co- and post translational of the ...
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Stable replication of the EBNA1/OriP mediated baculovirus vector and its application to anti HCV gene therapy
... using baculovirus-based shRNA- expressing vectors that contain the latent viral protein Epstein-Barr nuclear antigen 1 (EBNA1) and the origin of latent viral DNA replication (OriP) ...these ...
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Altered phosphorylation pattern of simian virus 40 T antigen expressed in insect cells by using a baculovirus vector.
... 4799 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The phosphorylation pattern of simian virus 40 SV40 large tumor T antigen purified from insect cells infected with [r] ...
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Intracellular localization and DNA-binding properties of human papillomavirus type 18 E6 protein expressed with a baculovirus vector.
... In mammalian cells, BPV-1 E6 protein is localized in equal proportions to both the nucleus and nonnuclear membranes 2, whereas the majority of cottontail rabbit papillomavirus CRPV E6 lo[r] ...
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Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the beta-galactosidase gene.
... 37-50 0022-538X/90/010037-14$02.00/0 Copyright C 1990, American Society for Microbiology Synthesis of the Membrane Fusion and Hemagglutinin Proteins of Measles Virus, Using a Novel Bacul[r] ...
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Expression and purification of biologically active v-sis/platelet-derived growth factor B protein by using a baculovirus vector system.
... The level of v-sis protein expressed in zinc-induced MMT-v-sis cells is the highest we have observed in mammalian cell transfectants when using a variety of promoters, including simian v[r] ...
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Efficient expression in insect cells of a soluble, active human insulin receptor protein-tyrosine kinase domain by use of a baculovirus vector.
... To determine whether this -48-kDa protein expressed in AchIRPTK 75.5-infected cells was indeed hIR PTK, cells were labeled metabolically at 48 h postinfection as described above, and cel[r] ...
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Expression of Aleutian mink disease parvovirus proteins in a baculovirus vector system.
... Coomassie blue-stained SDS-10% polyacrylamide gel A and immunoblot B analysis of total lysates of Sf9 cells infected with three different recombinant AcNPVs expressing the structural pro[r] ...
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Expression of polyomavirus large T antigen by using a baculovirus vector.
... An intronless construct of the PyTAg gene was obtained from plasmid pspLT5 28 by BamHI and XhoI digestion, purified by gel electrophoresis, and inserted into KpnI- and XhoI-digested pEV-[r] ...
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Nuclear assembly of polyomavirus capsids in insect cells expressing the major capsid protein VP1.
... As a test of the intrinsic in vivo assembly properties of a DNA virus coat protein, we expressed the polyomavirus capsid protein VP1 in insect cells using a baculovirus vector 9, 30 and [r] ...
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Synthesis of functional human immunodeficiency virus tat protein in baculovirus as determined by a cell-cell fusion assay.
... Abundant synthesis of functional human T-cell leukemia virus type I p4Y protein in eucaryotic cells by using a baculovirus expression vector.. A baculovirus vector can express intron-con[r] ...
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Surface displaying of swine IgG1 Fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction
... of baculovirus vectors displaying comple- ment interfering factors, of which a baculovirus vector displaying swine IgG1 Fc (pFc) showed the highest comple- ment antagonism ...the baculovirus ...
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Expression of Babesia equi Merozoite Antigen 1 in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme Linked Immunosorbent Assay
... EMA-1 was harvested and centrifuged at 100,000 ⫻ g for 2 h to remove the baculovirus. The supernatant was dialyzed against antigen coating buffer (0.05 M carbonate-bicarbonate buffer [pH 9.6]) and was then used ...
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Baculovirus Mediated Experimental Research on Targeted Egr1 Kringle 5 Gene Radiotherapy in Lung Adenocarcinoma
... the vector containing Egr1 promoter and its downstream K5 gene, an antiangiogenic agent gene, was constructed and tested by fluorescence microscopy for the ability of Egr1 promoter to regulate heterologous gene ...
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Site-Specific Integration in Mammalian Cells Mediated by a New Hybrid Baculovirus–Adeno-Associated Virus Vector
... with baculovirus replication since hybrid Bac- AAV viruses carrying the rep gene grow to titers comparable to those of other recombinant baculoviruses (data not ...upon baculovirus amplification in Sf9 ...
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Expression of Sindbis virus 26S cDNA in Spodoptera frugiperda (Sf9) cells, using a baculovirus expression vector.
... By immunoblot analysis with antisera directed against whole Sindbis virus and the individual structural proteins capsid, E2, and El, we have shown that polypeptides similar in size and a[r] ...
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Generation of mini-Tn7 transposone by cloning of CPV-VP2 into eukaryotic expression cassette, in E. coli
... The VP2 fragment, digested using BamHI/EcoRI restriction sites, was purified by low melting agarose gel using a DNA extraction kit (Vivantis-Korea). The purified product was ligated intopFastBac1 donor plasmid ...
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Site-specific glycosylation of the human cytomegalovirus tegument basic phosphoprotein (UL32) at serine 921 and serine 952.
... DISCUSSION The primary findings of this report are that i the full-length BPP from HCMV strain AD169 can be overexpressed as a recombinant baculovirus in Sf9 insect cells, ii baculovirus[r] ...
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Improved Adenovirus Type 5 Vector-Mediated Transduction of Resistant Cells by Piggybacking on Coxsackie B-Adenovirus Receptor-Pseudotyped Baculovirus
... Antibodies and proteins. Monoclonal anti-CAR antibody (clone E1.1 [19]) was obtained from Silvio Hemmi (University of Zu ¨rich, Zu ¨rich, Switzerland). The monoclonal anti-gp64 antibody clone AcV1 (Santa Cruz ...
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