Beta-galactosidase
Selective noncompetitive assimilation of bovine testicular beta galactosidase and bovine liver beta glucuronidase by generalized gangliosidosis fibroblasts
... liver beta-glucuronidase and testicular beta-galactosidase were assimilated by generalized gangliosidosis fibroblasts at respectively rates of 90 and 464 times the rate of assimilation of horseradish ...
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PURIFICATION OF BETA GALACTOSIDASE ENZYME FROM DAIRY EFFLUENT BACILLUS SPECIES
... Beta-galactosidases are the highly demanded enzymes in the diary industries for the hydrolysis of lactose into its constituent monosaccharides. Thermostable beta-galactosidases producing Bacillus sp D4 can ...
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The 67 kD elastin/laminin binding protein is related to an enzymatically inactive, alternatively spliced form of beta galactosidase
... of beta-galactosidase produced by alternative splicing demonstrates immunological and functional similarity and sequence homology to the 67-kD EBP, suggesting that the two might be the ...human ...
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BETA-GALACTOSIDASE AND BETA-GLUCURONIDASE ACTIVITIES IN INTESTINAL MUCOSA OF INFANTS AND CHILDREN
... fibrosis ( Table II ) showed a mean value. for lactase of 18.0 as compared to the con-[r] ...
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Effect of genomic location on expression of beta-galactosidase mRNA controlled by the herpes simplex virus type 1 UL38 promoter.
... Finally, we used deletional analysis to demonstrate that no more than 29 bases of DNA sequence 5' of the mRNA cap site are required for promoter activity in either location; this result [r] ...
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Three novel beta-galactosidase gene mutations in Han Chinese patients with GM1 gangliosidosis are correlated with disease severity
... Both c.495_497delTCT and p.G494V mutations identi- fied in patient 2 led to absence of GLB1 enzyme activity in transient expression studies, correlating with lack of in vivo residual enzyme activity (<1%). Two ...
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Production of Transgenic Chickens to Express Bacterial beta-Galactosidase and the Subsequent Utilization of Lactose as a Feed Stuff
... Endogenous lactase activity in chickens is very low. Only trace amounts of lactase activity can be detected at brush border membranes. Utilization of dietary lactose in chickens is limited to bacterial fermentation. ...
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Construction of fowlpox virus vectors with intergenic insertions: expression of the beta-galactosidase gene and the measles virus fusion gene.
... Each of the two plasmids generated had a BamHI site at the 3' end of the second promoter into which the 1.9-kbp measles virus gene encoding the fusion protein 8, 11 was inserted.. First,[r] ...
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Vaccinia virus recombinants expressing an 11-kilodalton beta-galactosidase fusion protein incorporate active beta-galactosidase in virus particles.
... When we examined p-galactosidase p-gal synthesis in cells infected with recombinant virus containing a late promoter region upstream of the lacZ gene, we observed that p-gal synthesis wa[r] ...
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Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the beta-galactosidase gene.
... 37-50 0022-538X/90/010037-14$02.00/0 Copyright C 1990, American Society for Microbiology Synthesis of the Membrane Fusion and Hemagglutinin Proteins of Measles Virus, Using a Novel Bacul[r] ...
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Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene.
... "Numbers of blue cells per milliliter of cell-free virus stock after infection of the CD4-LTR/,B-gal indicator cells.. 102 I LTRI/-gal indicator cells.[r] ...
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A herpes simplex virus mutant in which glycoprotein D sequences are replaced by beta-galactosidase sequences binds to but is unable to penetrate into cells.
... To determine whether F-gD, virions lacking gD could initiate certain of the initial stages of virus replication, namely induction of early viral polypeptides, Vero cells were infected wi[r] ...
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Transgenic mice ubiquitously expressing human placental alkaline phosphatase (PLAP): an additional reporter gene for use in tandem with beta galactosidase (lacZ)
... In recent years, the use of reporter genes in transgenic mice has underpinned much of the analysis of gene expression. The primary requirements of reporter genes are that their gene products are readily detectable, with ...
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Use of simian virus 40 large T-beta-galactosidase fusion proteins in an immunochemical analysis of simian virus 40 large T antigen.
... Inhibition of binding of '25I-labeled pure large T antigen to the immobilized antibody in the presence of the fusion protein cell extract containing ,-galactosidase alone was used as a c[r] ...
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Activation of a beta-galactosidase recombinant provirus: application to titration of human immunodeficiency virus (HIV) and HIV-infected cells.
... They are as follows: i direct titration of viral particles, ii direct titration of cells producing the virus and/or expressing a provirus, and iii indirect detection of the virus followi[r] ...
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Beta galactosidase activity in psychrotrophic microorganisms and their potential use in food industry
... Twenty-one psychrotrophic resp. psychrophilic bacterial strains were screened for presence of β-galactosidase activity which showed 8 of them. β-Galactosidase activity of these strains was determined for 2 ...
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LEUKOCYTE BETA-GALACTOSIDASE ACTIVITY IN THE DIAGNOSIS OF GENERALIZED GM, GANGLIOSIDOSIS
... Enzymatic activity in the mucopolysac- charidoses patients showed a mean activity of 6.7 which falls within the normal range. (5.3 to 11.8)[r] ...
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Assembly of gag-beta-galactosidase proteins into retrovirus particles.
... In any case, the physical presence of p-gal proteins in the supernatant of Psi2 cells expressing the GBG 1560 construct correlates with our results on p-gal activities Table 1 and indica[r] ...
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Beta-Galactosidase Production from Streptococcus thermophilus Isolated from Milk Yoghurt
... In Lactobacillus delbrueckii subsp. bulgaricus as well as in Streptococcus thermophilus, lactose is known to be transported by the secondary transport system. These lactose transporters turn out to be specific not only ...
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Effects of amino acid substitutions at the active site in Escherichia coli beta-galactosidase.
... This approach to protein engineering has been employed previously, using five of the naturally occurring amber suppres- sors (MILLER et al. T h e addition of seven [r] ...
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