bipartite nuclear localization signal
The human T-cell lymphotropic virus type 1 Tof protein contains a bipartite nuclear localization signal that is able to functionally replace the amino-terminal domain of Rex.
... T-antigen signal to direct binding to or activation of the RXRE provides indirect evidence that prop- erties observed for the Tof NLS are indeed specific and rele- vant to the function of the ...
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Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport.
... in nuclear transport ...complete nuclear translocation of the protein ...background nuclear staining seen with b -Gal alone was found ...the bipartite config- uration was necessary for ...
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The Bovine Immunodeficiency Virus Rev Protein: Identification of a Novel Lentiviral Bipartite Nuclear Localization Signal Harboring an Atypical Spacer Sequence
... Rev nuclear export activity was quantified in transient-transfection assays using a pDM138-based BIV Rev chloramphenicol acetyltransferase (CAT) reporter ...
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Characterization of the Interaction between the Interferon-Induced Protein P56 and the Int6 Protein Encoded by a Locus of Insertion of the Mouse Mammary Tumor Virus
... of nuclear Int6 upon coexpression of ...a nuclear protein, we investigated the structural basis of this ...a bipartite nuclear localization signal and a nuclear export ...
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Human foamy virus Bel1 transactivator contains a bipartite nuclear localization determinant which is sensitive to protein context and triple multimerization domains.
... the nuclear tar- geting signal of Bel1 is bipartite, comprising two interdepen- dent clusters of basic amino acids separated by an intervening ...in nuclear transport, or one of the two basic ...
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Differential Intracellular Compartmentalization of Herpetic Thymidine Kinases (TKs) in TK Gene-Transfected Tumor Cells: Molecular Characterization of the Nuclear Localization Signal of Herpes Simplex Virus Type 1 TK
... Many nuclear targeting sequences have been ...for nuclear localization signals (for an overview, see reference 23): (i) they usually consist of short amino acid sequences, contain- ing a high ...
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Biologic and Immunologic Effects of Knockout of Human Cytomegalovirus pp65 Nuclear Localization Signal
... prototypic nuclear localization signal (NLS) in which arginine and lysine predominate within a bipartite motif in which short regions of basic amino acids are separated by 10 or more ...
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A nuclear targeting system in Plasmodium falciparum
... a bipartite signal sequence to facilitate apicoplast targeting and membrane translocation ...a nuclear targeting technique in ...a nuclear carrier ...A nuclear localiza- tion ...
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The ORF012 Gene of Marek's Disease Virus Type 1 Produces a Spliced Transcript and Encodes a Novel Nuclear Phosphoprotein Essential for Virus Growth
... control nuclear import was provided by our infection experiments using a set of mutant viruses carrying mutations in the ...virus, nuclear localization was completely abrogated in cells infected with ...
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Identification of nuclear localization signal and nuclear export signal of VP1 from the chicken anemia virus and effects on VP2 shuttling in cells
... subcellular localization of viral proteins, especially in the nucleus, may contribute to cell apoptosis, viral replication or host cell proliferation [9, ...the nuclear localization signal ...
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The role of nuclear localization signal in parvovirus life cycle
... Five BRs were found in the VP1u of porcine parvovirus (PPV), named BR1 (3PPAKRAR9), BR2 (86RAKR89), BR3 (110RRSPRK115), BR4 (124KR125), and BR5 (133KKKAK137) [35] (Fig. 1b iii). Both BR1 and the combination of BR4 and ...
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Functional Characterization of the Nuclear Localization Signal for a Suppressor of Posttranscriptional Gene Silencing
... Geminiviruses are a family of unique small circular single- stranded DNA (ssDNA) viruses that replicate via double- stranded (ds) DNA intermediates by a rolling circle mecha- nism in plant cell nuclei (18). The ...
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Identification of a non-canonical nuclear localization signal (NLS) in BRCA1 that could mediate nuclear localization of splice variants lacking the classical NLS
... non-classical signal sequences of experimentally proven nuclear ...a nuclear localization signal, we constructed two different fusion proteins using the vector enhanced green ...
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Interaction of the Vp3 Nuclear Localization Signal with the Importin α2/β Heterodimer Directs Nuclear Entry of Infecting Simian Virus 40
... Mutant NO-SV40 DNA was then transfected into the cells, and DNA that had entered the nucleus was allowed to initiate a single infection cycle. Provided that the mutation did not affect virion assembly in the nucleus, ...
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Nuclear Import of Bovine Papillomavirus Type 1 E1 Protein Is Mediated by Multiple Alpha Importins and Is Negatively Regulated by Phosphorylation near a Nuclear Localization Signal
... aberrant nuclear envelope accumulation in transfected HeLa ...E1 nuclear uptake and are con- sistent with a previous study that examined the replication function of serine 109 mutants ...E1 nuclear ...
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Inhibition of HIV 1 integrase nuclear import and replication by a peptide bearing integrase putative nuclear localization signal
... This nuclear translocation machinery is highly conserved among lower and higher eukaryotes ...the nuclear envelope via the NPC ...the nuclear import of the HIV genome ...the nuclear import ...
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A Nuclear Localization Signal in Herpesvirus Protein VP1-2 Is Essential for Infection via Capsid Routing to the Nuclear Pore
... Growth characteristics of K.VP1-2 ⌬ NLS. We previously identi- fied a short, highly conserved basic motif in VP1-2, embedded within an otherwise poorly conserved region at the N terminus of the protein (Fig. 1a). We ...
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Regions of the Varicella-Zoster Virus Open Reading Frame 63 Latency-Associated Protein Important for Replication In Vitro Are Also Critical for Efficient Establishment of Latency
... the nuclear localization signal at residues 260 to 263, plasmid 63-BclI was cut with AatII (which cuts at VZV nucleotide 111366) and the linearized DNA was treated with Bal 31 nuclease for 15 to 60 ...
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The bovine papillomavirus type 1 E2 transactivator and repressor proteins use different nuclear localization signals.
... the nuclear membrane, and into the nucleus. Several nuclear transport models have been proposed (reviewed in references 10 and ...the nuclear pores, which has been estimated to be between 40 and 60 ...
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Nuclear Localization Signal of HIV-1 as a Novel Target for Therapeutic Intervention
... (1992) Active nuclear import of human immunodeficiency virus type 1 preintegra- tion complex. Stevenson M, Bukrinsky M, Haggerty S[r] ...
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