BMV, brome mosaic virus
Complete genome sequence of a Brome Mosaic Virus isolate from the Czech Republic
... between BMV-CZ and the Russian isolate (up to ...the BMV-CZ isolate with the other BMV isolates highlighted that diversity was distributed along the genomes of RNA-1 and RNA-2 (Table 1 and Figure ...
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Enhancer-Like Activity of a Brome Mosaic Virus RNA Promoter
... in BMV mi- nus-strand synthesis is provided by SLC, which features a clamped adenine ...the BMV replicase, a single-stranded sequence is ...the BMV minus-strand ...
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Phosphorylation of the Brome Mosaic Virus Capsid Regulates the Timing of Viral Infection
... the BMV CP could affect the localization of the N-terminal arm inside and outside the ...the BMV genome (Fig. 6D). The BMV CP has been shown to directly contact these RNA motifs to differentially ...
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Brome Mosaic Virus Polymerase-Like Protein 2a Is Directed to the Endoplasmic Reticulum by Helicase-Like Viral Protein 1a
... As shown by representative images of such cells in Fig. 3A, 1a and GFP-2a displayed almost perfect colocalization, usually in the shape of a partial or complete ring. DNA staining (Fig. 3A) showed that these rings ...
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Brome Mosaic Virus RNA Syntheses In Vitro and in Barley Protoplasts
... 6. BMV RNA replication and RNA synthesis by the BMV replicase as a function of ...with BMV RNAs synthesized in transfected ...minimal BMV promoter templates used to assess minus-strand RNA and ...
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Structure-based rationale for the rescue of systemic movement of brome mosaic virus by spontaneous second-site mutations in the coat protein gene.
... In our experiments we used a variety of C. hybridum which has green stems and petioles. This green variety supports both local lesion formation and systemic spread of BMV (16). In contrast, the variety with purple ...
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Mutations in the Capsid Protein of Brome Mosaic Virus Affecting Encapsidation Eliminate Vesicle Induction In Planta: Implications for Virus Cell-to-Cell Spread
... wt BMV CP in ...wt BMV and those infiltrated with the empty vector (pCASS) alone served as appropriate posi- tive and negative controls, ...of BMV could be purified from leaves expressing wt B4, ...
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Putative RNA Capping Activities Encoded by Brome Mosaic Virus: Methylation and Covalent Binding of Guanylate by Replicase Protein 1a
... isolation. BMV 1a protein expressed in the yeast ...complete BMV RNA replication and subgenomic mRNA syn- thesis in vivo ...other BMV components were spheroplasted, lysed, and total membranes were ...
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Subcellular Localization and Rearrangement of Endoplasmic Reticulum by Brome Mosaic Virus Capsid Protein
... licated BMV progeny RNA was first detected (Fig. 2A), accu- mulation of a large cluster of vesicles into membrane sacs, which we refer to as vesicle packets (VPs), was readily ob- served (Fig. 2E). These VPs are ...
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Emergence of Distinct Brome Mosaic Virus Recombinants Is Determined by the Polarity of the Inoculum RNA
... (52), BMV replication is asymmetric, resulting in a (⫹)/(⫺)-strand ratio of 100:1 (30, ...robust BMV replication can be initiated as early as 2 dpi (10); and (iv) BMV infection initiated by ...
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Analysis of the interaction of viral RNA replication proteins by using the yeast two-hybrid assay.
... The brome mosaic virus (BMV) system has been characterized extensively both genetically and biochemically, providing numerous mutations in the BMV 1a helicase-like and 2a polymerase- ...
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A mathematical model of RNA3 recruitment in the replication cycle of Brome mosaic virus
... the brome mosaic virus (BMV) and hepatitis C virus, utilize a replication cycle which involves the recruitment of RNA genomes from the cellu- lar translation machinery to the viral ...
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Interactions between Brome Mosaic Virus RNAs and Cytoplasmic Processing Bodies
... of brome mo- saic virus (BMV), which has a tripartite segmented genome consisting of RNA1, RNA2, and RNA3 and normally infects ...The BMV life cycle has been shown to occur in the yeast ...
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Regulation of Brome Mosaic Virus Gene Expression by Restriction of Initiation of Protein Synthesis
... 330 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The translation of total and individual brome mosaic virus BMV RNAs was examined in a wheat germ cell-free system in[r] ...
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Biological activities of hybrid RNAs generated by 3'-end exchanges between tobacco mosaic and brome mosaic viruses.
... 3451 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Sequences within the conserved, aminoacylatable 3' noncoding regions of brome mosaic virus BMV genomic RNAs 1, 2, a[r] ...
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Recognition of the Core RNA Promoter for Minus-Strand RNA Synthesis by the Replicases of Brome Mosaic Virus andCucumber Mosaic Virus
... SLC-like sequence in CMV isolates within subgroup I. Un- like BMV, of which only a limited number of isolates have been identified, many CMV isolates have been identified and sequenced. Based on serological ...
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Analysis of the role of brome mosaic virus 1a protein domains in RNA replication, using linker insertion mutagenesis.
... 6110 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Brome mosaic virus BMV belongs to a "superfamily" of plant and animal positive-strand RNA viruses that share, among[r] ...
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In vivo DNA expression of functional brome mosaic virus RNA replicons in Saccharomyces cerevisiae.
... on BMV RNA replication. The potential to initiate BMV RNA replication from DNA in vivo was first illustrated by Mori et ...that BMV cDNAs fused to the cauliflower mosaic virus 35S ...
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Bromovirus RNA Replication Compartment Formation Requires Concerted Action of 1a's Self-Interacting RNA Capping and Helicase Domains
... vesicles. Brome mosaic virus (BMV) RNA synthesis occurs in vesicular endoplasmic reticulum (ER) mem- brane invaginations, each induced by many copies of viral replication protein 1a, which has ...
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Deletion of the C-terminal 33 amino acids of cucumber mosaic virus movement protein enables a chimeric brome mosaic virus to move from cell to cell.
... of BMV by creating and testing chimeric viruses. BMV and CMV are taxonomically related (both are members of the family Bromoviridae), and the MPs of BMV and CMV show significant amino acid sequence ...
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