Bordetella species
Evaluation of a Multitarget Real Time PCR Assay for Detection of Bordetella Species during a Pertussis Outbreak in New Hampshire in 2011
... hIS1001, pIS1001, and ptxS1 targets. The PCR results of these nine specimens were interpreted as indeterminate. High occurrence (18.6%) of indeterminate PCR results in Bordetella PCR testing has been reported ...
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Comparative study of hemolytic activity of Bordetella species
... bulbs. Bordetella species were isolated from the specimen (n=50) collected from nasopharynx region of suspected whooping cough patients using calcium alginate swabs during the period of April 2006 to April ...
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Evaluation of Level of Agreement in Bordetella Species Identification in Three U S Laboratories during a Period of Increased Pertussis
... detecting Bordetella pertussis in the United States, most laboratories use inser- tion sequence 481 (IS481), which is not specific for ...other Bordetella species is not ...other Bordetella ...
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Novel Multitarget Real Time PCR Assay for Rapid Detection of Bordetella Species in Clinical Specimens
... Coinfections of B. parapertussis and B. pertussis have been documented (14). In our clinical specimens, two coinfections of B. pertussis and B. parapertussis were potentially identified, with IS481 C T values of less ...
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Comparison of Western Immunobloting to an Enzyme-Linked Immunosorbent Assay for the Determination of Anti-Bordetella pertussis Antibodies
... anti-PT antibodies are highly specific for B. pertussis infection (18, 20). Anti-FHA antibodies are less specific than PT anti- bodies and may be produced following infection with Myco- plasma pneumoniae, Chlamydia ...
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Evaluation of the Aries Bordetella Assay for Detection and Identification of Bordetella pertussis in Nasopharyngeal Swab Specimens
... Aries Bordetella assay (Aries BA) uses Luminex Corporation’s MultiCode real-time PCR chemistry in combination with the Aries ...Aries Bordetella assay can directly detect and differentiate ...Aries ...
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Laboratory diagnosis of pertussis: state of the art in 1997
... PCR results with clinical samples. The results obtained by various investigators with different PCR systems performed with clinical samples are presented in Table 1. All PCR meth- ods proved to be very specific, although ...
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The BvgAS Regulon of Bordetella pertussis
... in Bordetella species have identified numerous BvgA-regulated ...both species in the Bvg( ⫹ ) mode, while more species-specific differences were observed in the Bvg( ⫺ ) ...a Bordetella ...
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Bordetella holmesii sp nov , a new gram negative species associated with septicemia
... from Bordetella species and other phenotypically similar bacteria are listed in Table ...Acinetobacter species and CDC group NO-1 ...Acinetobacter species that produce a brown soluble pig- ...
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Harmonization of Bordetella pertussis Real Time PCR Diagnostics in the United States in 2012
... detect Bordetella species in nasopharyngeal swabs or aspirates without the added complication of multiple laboratories and PCR proto- cols will be required to definitively resolve this ...
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Immunomagnetic separation and solid phase detection of Bordetella pertussis
... In the present study, novel solid-phase methods were used for both sample preparation and PCR detection of Bordetella pertussis. The sample preparation was performed by immunomagnetic separation with paramag- ...
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Evaluation of PCR for Diagnosis of Bordetella pertussis and Bordetella parapertussisInfections
... The present study showed that PCR, using the primers PIp1 and PIp2, was specific for B. pertussis with one exception: B. holmesii gave rise to a band indistinguishable from that in- duced by B. pertussis. This band also ...
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Prevalence and Sequence Variants of IS481 in Bordetella bronchiseptica: Implications for IS481 Based Detection of Bordetella pertussis
... the PvuII ribotype of MBORD669 is unique among our IS481- positive isolates (40; K. B. Register, unpublished data). A com- parative evaluation of MBORD669 DNA obtained from each laboratory could perhaps assist in ...
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Evaluation of Real Time PCR for Detection of and Discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for Clinical Diagnosis
... The clinical significance of detecting B. holmesii is not yet fully understood, but the bacterium has been isolated from the respiratory tract (16, 35) and been reported to cause disease (24). However, because of the ...
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Nucleic Acid Amplification Tests for Diagnosis of Bordetella Infections
... other Bordetella species may require a genus-specific real-time PCR format, followed by a species-specific ...and Bordetella PCR will be just one of many applications to be run on mech- anized ...
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Hemagglutination by Bordetella bronchiseptica
... A total of 53 isolates of Bordetella bronchiseptica from dogs and pigs were tested for their ability to agglutinate chicken, horse, sheep, dog, pig, and guinea pig erythrocytes.. No diff[r] ...
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Multiplex LightCycler PCR Assay for Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis in Nasopharyngeal Specimens
... differentiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was ...for Bordetella culture and/or direct fluorescent antibody testing and a second LightCycler PCR assay ...
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Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.
... The regions of IS481 and BP0026, targeted in our assay are sensitive with LLOD of 1 and 10 B. per- tussis genomic equivalent per reaction, respectively. The region of IS1001 targeted in our assay is sensitive with LLOD ...
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Comparison of Ribotyping and Restriction Enzyme Analysis for Inter and Intraspecies Discrimination of Bordetella avium and Bordetella hinzii
... sheep and turkey erythrocytes by B. avium and B. hinzii and found that the outcome is affected by the method used (Reg- ister, unpublished data). Most B. hinzii isolates are weakly positive when a 96-well plate procedure ...
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Maternal and infant Bordetella pertussis infection
... The diagnosis of B. pertussis infection in the mother was confirmed on day 19 of her illness by the detection of B. pertussis IgM and IgA antibodies in serum.. The diagnosis was [r] ...
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