Brain slices
A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
... Experimental papers often do not report about slice selection. Some researchers have pointed to difficulties, e.g., variation in PI-uptake, incomplete layers or pre- damage, but did not explain objective criteria ...
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L Serine Decreases Taurine Concentration in the Extracellular Fluid of Brain Slices
... L-Serine is considered a functional amino acid in the central nervous system, and induces sedation and hypnotic effects in some animal models of acute and chronic stress. Accordingly, while L-serine is a candidate ...
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Assessment of glutamatergic synaptic transmission and plasticity in brain slices: relevance to bioelectronic approaches
... and brain biochemistry for the imple- mentation of bioelectronic approaches in the ...hippocampal slices and electrophysiological ...Therefore, brain slices are a good system in which to ...
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Effects of anoxia on energy metabolism in crucian carp brain slices studied with microcalorimetry
... The slices were in good condition energetically, since after 20 h, their energy charge and adenylate levels were not significantly different from the in situ ...Turtle brain slices (Doll et ...only ...
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Targeting neurotransmitter receptors with nanoparticles in vivo allows single molecule tracking in acute brain slices
... organotypic slices, which provide the great advantage of an easy and direct access to superficial cells 15 ...acute brain slices has thus been a major challenge that has bogged down our understanding ...
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Organotypic brain slices: a model to study the neurovascular unit micro-environment in epilepsies
... Due to the presence of all cell types and their interactions, preservation of TJs between endothelial cells, as well as BBB carriers and transporters, brain slices provide a complete ex vivo model of the ...
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Plastination and Staining of Brain Slices Using Two Different Dehydration Methods
... [6]. Brain slices plastinated by the silicon10 (S10) technique require macroscopic staining to differentiate between fiber tracts (white matter) and cell bodies (gray ...studies, brain slices ...
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Role of Pre-Synaptic NMDA Receptors in the Modulation of Inhibitory Synaptic Transmission in Sensory-Motor and Visual Cortical Pyramidal Neurons in Brain Slices of Young Epileptic Mice
... human years (2, 3). A rat model (3) correlates with human years for the entire life span as 13.2 days in the rodent equals to one year in humans (13.2 rat days = one year human). The correlation is simply derived from ...
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Dense arrays of micro-needles for recording and electrical stimulation of neural activity in acute brain slices
... acute slices of brain tissue. Approach. Acute slices, arguably the closest in-vitro model of the brain, have a damaged surface ...cortical slices with a signal to noise ratio of up to ...
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A New Abnormality Detection Approach for T1-Weighted Magnetic Resonance Imaging Brain Slices Using Three Planes
... MRI slices have a similar technical specification because all of them for each patient case are obtained from the same MRI ...of slices. Thus, it is necessary to choose only those slices that contain ...
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Investigation of muscarinic and metabotropic (glutamate) response properties of adult and immature olfactory cortical brain slices in vitro.
... animal slices to mimic the effect of downregulation, and to assess a possible role in epileptogenesis in conjunction with ...the slices with 1 pM CGP 52432 alone resulted in an inhibition of the GABAg ...
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Optimized heterologous transfection of viable adult organotypic brain slices using an enhanced gene gun
... the brain imbedded in the medium- agarose matrix becomes easier to slice, and these subse- quent OTBS can easily be manipulated and this maintains the regional brain anatomy ...our slices indicated ...
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Sensory input is required for callosal axon targeting in the somatosensory cortex
... P9 brain slices prepared from mice with P5 unilateral or simultaneous bilateral removal of sensory ...vitro. Brain slices were incubated with 50 mM KCl for 10 min and c-Fos staining were ...
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Neuronal activity mediated regulation of glutamate transporter GLT-1 surface diffusion in rat astrocytes in dissociated and slice cultures.
... hippocampal brain slices, which exhibit functional local synaptic circuitry and preserved brain ...in slices, which could restrict GLT-1 ...organotypic slices, possibly due to a high ...
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Targeting the purine salvage pathway in in vitro models of cerebral ischemia
... Due to the high activities of ATPases in fresh samples, changes in the adenine nucleotide levels take place very quickly when ATPase enzymes are not inactivated immediately. Snap-freezing of tissues in liquid nitrogen is ...
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No oxygen? No problem! Intrinsic brain tolerance to hypoxia in vertebrates
... isolated brain slices from naked mole- rats are remarkably tolerant of acute hypoxic ...in brain slices from naked mole- rats compared with age-matched mice (Peterson et ...by slices ...
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The influence of heterocyclic compound-PAMAM dendrimer complexes on evoked electrical responses in slices of hypoxic brain tissue
... in brain tissue would affect the functioning of its signaling systems under hypoxic ...hippocampal slices as the in vitro model to test our ...in brain slices and in cell culture [10, ...
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A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578
... neurons, iPSC-CMs, neurons in organotypic rat brain slices, zebrafish RB cells, and mouse V1 neurons in vivo. Though not yet ideal by all criteria, K-GECO1 represents a step forward in the development of ...
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Daria, Anna (2018): Microglial phagocytosis of amyloid plaques in an ex vivo model of Alzheimer's Disease. Dissertation, LMU München: Medizinische Fakultät
... AD brain, their involvement in amyloid plaque clearance over the course of AD is still ...organotypic brain slices from aged, amyloid plaque-bearing mice (APPPS1) together with slices from ...
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A New Method of Brain Plastination
... plastinated brain sections with ...the brain introduced by Weber and Henry produced more detailed, durable, and easier to handle specimens with 4 mm thickness by P35 technique ...plastinated brain ...
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