Broad-host-range plasmids
Destruction of Escherichia coli and Broad Host Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions
... of broad-host-range plasmid and chromosomal DNA in simulated ...IncP broad-host-range plasmids (trfA) were monitored using real-time quantitative polymerase chain reaction ...
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Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory
... expression plasmids constructed in this study are shown in Table ...RK2 broad host range plasmid-based chassis developed by the group of Victor de Lorenzo (in preparation) carrying either the ...
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Oncoretrovirus and Lentivirus Vectors Pseudotyped with Lymphocytic Choriomeningitis Virus Glycoprotein: Generation, Concentration, and Broad Host Range
... Recombinant oncoretroviral and lentiviral vector particles with LCMV-GP were compared with the widely used A-MLVenv- and VSV-G-pseudotyped vectors. Vector titers after transient production were in the same order of ...
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Broad host range of human T-cell leukemia virus type 1 demonstrated with an improved pseudotyping system.
... Transfections of plasmids were done as described in the text. pHTLV-1-env- rre is driven by the SV40 origin-promoter (18), and pcDNA3-HTLV-1-env-rre has the cDNA insert from pHTLV-1-env-rre driven by the ...
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Development of a gene silencing DNA vector derived from a broad host range geminivirus
... the host cell nucleus through dsDNA ...very broad host range that includes many important crop ...the host range of individual begomoviruses is quite ...genome plasmids ...
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Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production
... Plasmids used as templates or constructs that were gen- erated in this study are listed in Table 1. DNA sequences of the PCR primers used during cloning steps described below are presented in Table 2. Plasmid ...
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Genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain NZP2037
... a broad host range and is one of very few ...any plasmids and contains a single scaffold of size 7,462,792 bp which encodes 7,318 protein-coding genes and 70 RNA-only encoding ...
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Characteristics of PRD1, a Plasmid-Dependent Broad Host Range DNA Bacteriophage
... However, these results, when considered with those in Table 2, show that the host range of PRD1 extends beyond a single compatibility group and that those plasmids that confer PRD1 sensi[r] ...
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Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants
... tech, Palo Alto, CA, U.S.A.), pECFP (Clontech), and pEYFP (Clontech) using unique XbaI and BamHI restriction sites into similarly digested pBBR1MCS-5 (Kovach et al. 1995). The en- suing plasmids (pMP2444 and ...
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Small-Molecule Probes Targeting the Viral PPxY-Host Nedd4 Interface Block Egress of a Broad Range of RNA Viruses
... with plasmids expressing either mVP40 or eVP40 in the absence (DMSO alone) or presence of compound 2 ...and host GAPDH in cell extracts ...and host GAPDH in cell extracts ...
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Identification of virulence associated loci in the emerging broad host range plant pathogen Pseudomonas fuscovaginae
... Biosciences); plasmids were puri- fied using the EuroGold plasmid columns (Euro Clone) or with the alkaline lysis method [67]; total DNA from Pfv strains were isolated by Sarkosyl/Pronase lysis as described ...
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Complete Genome sequence of Burkholderia phymatum STM815T, a broad host range and efficient nitrogen-fixing symbiont of Mimosa species
... The genome includes two chromosomes and two plasmids, for a total size of 8,676,562 bp (62.3% GC content). Chromosome 1 is 3.48 Mb in size (63.0% GC), chromosome 2 is 2.69 Mb (62.3% GC), plasmid 1 is 1.90 Mb ...
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Broad Host Range Expression Reveals Native and Host Regulatory Elements That Influence Heterologous Antibiotic Production in Gram Negative Bacteria
... cloning, broad-host-range expression vector for proteobacterial ...Gram-negative broad-host-range ele- ments for heterologous expression ...1A). ...
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Triterpenoids Manipulate a Broad Range of Virus-Host Fusion via Wrapping the HR2 Domain Prevalent in Viral Envelopes
... China). Plasmids expressing the wild-type HIV-1 envelope protein were obtained by inserting HIV-1 gp160 cDNA (strain NDK) between the KpnI and XbaI sites of the PCMV3 mammalian expression ...Other plasmids ...
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An Analysis of Emotional Maturity of Adolescent students in relation to Parent-Child Interaction of Fazilka District (Punjab)
... its host bacterium but also was able to infect ...a broad host range of ...a range of bacterial species ...of broad- host- range bacteriophages ...
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Genome sequence of the clover-nodulating Rhizobium leguminosarum bv. trifolii strain TA1
... a broad host range for nodulation and nitrogen fixation across annual and perennial clovers originating from the European and Mediterranean centre of origin of clovers ...
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Broad host range species in specialised pathogen groups should be treated with suspicion – a case study on Entyloma infecting Ranunculus
... a broad host range, encom- passing 30, 26, and 5 species of Ranunculus, ...This broad host range is in contrast to the generally high host specificity assumed for species ...
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Development of a system of genetic analyses for Rhodomicrobium vannielii
... the plasmids of the Rhodospirillaceae are consistent with those of conjugative plasmids (generally >20 ...indigenous plasmids has not been demonstrated. If such plasmids are shown to ...
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Characteristics and Purification of PRR1, an RNA Phage Specific for the Broad Host Range Pseudomonas R1822 Drug Resistance Plasmid
... Characteristics and Purification of PRR1, an RNA Phage Specific for the Broad Host Range Pseudomonas R1822 Drug Resistance Plasmid Received for publication 17 July 1973 A new drug resist[r] ...
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Promoters of the broad host range plasmid RK2: analysis of transcription (initiation) in five species of gram-negative bacteria.
... Using this system, we cloned 12 DNA fragments with promoter activity from the broad host range plasmid RK2 and measured their activities in five species of Gram-n[r] ...
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