Broad-range PCR
Broad range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian patients with reactive and undifferentiated arthritis
... for broad-spectrum PCR to detect bacterial DNA in SF samples allowing the identification of the species ...the PCR-positive samples revealed the presence of a wide spectrum of bacterial DNA in SF ...
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High genotypic diversity and a novel variant of human cytomegalovirus revealed by combined UL33/UL55 genotyping with broad range PCR
... developed PCR assays with degenerate primers targeting two variable HCMV genes, glycoprotein B (gB, UL55) and the G- protein-coupled receptor gene ...variants, PCR assays were supplemented with ...
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Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad range PCR, cloning and sequencing
... Propionibacterium acnes sequences were detected in syno- vial samples of one ReA patient with pustular lesions and two UA patients in whom we did not detect any other causative bacteria derived DNA. Thus, the presence of ...
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Real Time Quantitative Broad Range PCR Assay for Detection of the 16S rRNA Gene Followed by Sequencing for Species Identification
... by broad-range PCR allows subsequent identification of the or- ganism, as the PCR product contains variable bacterial species- specific ...
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Identification of Bacterial Pathogens in Ascitic Fluids from Patients with Suspected Spontaneous Bacterial Peritonitis by Use of Broad Range PCR (16S PCR) Coupled with High Resolution Melt Analysis
... published broad-range PCR (16S PCR) coupled with high-resolution melt analysis (HRMA) was compared with standard culture techniques for diagnosis of SBP in 106 peritoneal fluid samples from ...
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Comparison of two commercial broad range PCR and sequencing assays for identification of bacteria in culture negative clinical samples
... A broad-range PCR assay was implemented seven years ago in the department in order to identify pathogens in culture-negative samples from patients with a suspected ...by broad-range 16S ...
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Prosthetic Joint Infection Diagnosis Using Broad Range PCR of Biofilms Dislodged from Knee and Hip Arthroplasty Surfaces Using Sonication
... Broad-range PCR is a very sensitive method, prone to false- positive results from contaminating ...other PCR reagents may be contaminated with bacterial DNA ...inhibit PCR. Despite ...
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Molecular diagnosis of bacterial endocarditis by broad range PCR amplification and direct sequencing
... conclusion, broad-range PCR amplification followed by direct sequencing is a relatively easy and reliable method that gives accurate results when applied to surgically removed heart valves of ...
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Comparative Analysis of Two Broad Range PCR Assays for Pathogen Detection in Positive Blood Culture Bottles: PCR–High Resolution Melting Analysis versus PCR Mass Spectrometry
... wide range of species- specific internal segments can be amplified using relatively few primer pairs ...differentiate PCR products for pathogen diagnosis but can be slow and is prone to contamination, which ...
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Broad Range PCR Electrospray Ionization Mass Spectrometry for Detection and Typing of Adenovirus and Other Opportunistic Viruses in Stem Cell Transplant Patients
... real-time PCR tests, mainly for viruses in the families Herpesviridae and Adenoviri- dae that contribute to a high morbidity, especially in pediatric ...combines broad- range PCR with ...
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Broad Range PCR Coupled with Electrospray Ionization Time of Flight Mass Spectrometry for Detection of Bacteremia and Fungemia in Patients with Neutropenic Fever
... and PCR setup were automatically performed by a single instrument using prefilled individual disposable sample preparation cartridges and prefilled 16-well PCR ...a broad range of bacteria and ...
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Optimal DNA Isolation Method for Detection of Bacteria in Clinical Specimens by Broad Range PCR
... bacterial PCR were divided in ...rDNA-targeted PCR described above was used to screen for the presence of bacteria in the ...rDNA PCR-positive sample was identified by sequencing the 16S and/or 23S ...
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Risk Assessment Models and Contamination Management: Implications for Broad Range Ribosomal DNA PCR as a Diagnostic Tool in Medical Bacteriology
... eubacteria. Broad-range PCR primers may then be designed to recognize these conserved bacterial 16S rRNA gene sequences and used to amplify inter- vening, variable, or diagnostic regions (15, ...17). ...
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First report of Sneathia sanguinegens together with Mycoplasma hominis in postpartum prosthetic valve infective endocarditis: a case report
... Negative blood cultures but positive 16S rRNA broad-range PCR in valve material and/or serology has previously been published in M. hominis IE [5–7]. Sneathia sanguinegens (formerly known as ...
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Identification and Differentiation of Clinically Relevant Mycobacterium Species Directly from Acid Fast Bacillus Positive Culture Broth
... incorporated broad-range PCR followed by suspension array hybridization to identify 17 clinically relevant mycobacterial complexes, groups, and species in one single ...
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Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... a broad-range real-time PCR assay tar- geting clinical relevant fungal pathogens besides Candida and Aspergillus in one assay is currently not ...of broad-range PCR assays for ...
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Utility of PCR in Diagnosis of Invasive Fungal Infections: Real Life Data from a Multicenter Study
... of broad-range PCR using the internal transcribed spacer region (ITS) for diag- nosis of microscopy-negative fungal infections in nonselected patient populations are ...gene PCR compared with ...
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Monitoring of Patients Supported by Extracorporeal Membrane Oxygenation for Systemic Infections by Broad Range rRNA Gene PCR Amplification and Sequence Analysis
... gene PCR followed by sequencing identifies the broadest range of pathogens ...a broad-range PCR test, SepsiTest (6, 10–14), for the monitoring of patients for DNA of pathogens in the ...
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Evaluation of Universal Probes and Primer Sets for Assessing Total Bacterial Load in Clinical Samples: General Implications and Practical Use in Endodontic Antimicrobial Therapy
... Access to the pulp chamber and sample collection (before and after endodontic procedures) were performed by the pro- tocol described by Jacinto et al. (6). The initial samples were collected and transported to the ...
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Detection of hydrogen peroxide producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV 1 seropositive women
... additional broad range PCR testing: one sample from each participant with ≤2 eligible samples (n = 12) and 4 from the participant with 9 eligible ...by broad range PCR is ...
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