Broadly neutralizing antibody
Predicting the broadly neutralizing antibody susceptibility of the HIV reservoir
... of antibody neutralization activity has been mainly used for vaccine design to develop immunogens for elicitation of bNAbs, and these models have primarily focused on Env protein genetic ...CD4bs antibody ...
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Honing a harder hitting hammerhead improves broadly neutralizing antibody breadth and potency
... lacking. Broadly neutralizing monoclonal antibodies (bnmAbs) with the capacity to neutralize multiple HIV-1 variants have been isolated from HIV-1–infected individuals, and there has been a great effort to ...
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The V1V2 Domain and an N-Linked Glycosylation Site in the V3 Loop of the HIV-1 Envelope Glycoprotein Modulate Neutralization Sensitivity to the Human Broadly Neutralizing Antibody 2G12
... Molecular modeling of gp120 monomers and trimers. A three-dimensional (3D) model of the monomeric gp120 of 5008CL2 and 11005CL7 was built by comparative modeling techniques using the SwissModel modeling tool (http: ...
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Identification of broadly neutralizing antibody epitopes in the HIV 1 envelope glycoprotein using evolutionary models
... Our computational approach to identifying sites targeted by broadly neutralizing antibodies is an extension of the method of Lacerda et al. [42], which was originally devel- oped to predict T cell epitopes ...
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Cross-protective immune responses induced in rhesus macaques by immunization with attenuated macrophage-tropic simian immunodeficiency virus.
... correlated broadly neutralizing antibody responses to SIV and HIV-1 with conformation-de- pendent antibodies, we sought to examine the conformation dependence of envelope-specific antibody ...
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Engineering Recombinant Reoviruses To Display gp41 Membrane-Proximal External-Region Epitopes from HIV-1
... ABSTRACT The gp41 membrane-proximal external region (MPER) is a target for broadly neutralizing antibody responses against human immunodeficiency virus type 1 (HIV-1). However, replication-defective ...
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Structure-Based Design of Hepatitis C Virus Vaccines That Elicit Neutralizing Antibody Responses to a Conserved Epitope
... a broadly neutralizing antibody in complex with a conserved linear epitope from the HCV E2 envelope glycoprotein (residues 412 to 423; epitope I), we performed structure-based design of immunogens to ...
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Cell surface ectodomain integrity of a subset of functional HIV 1 envelopes is dependent on a conserved hydrophilic domain containing region in their C terminal tail
... only broadly neutralizing antibody (bNAb) epitopes while occlud- ing the epitopes targeted by non-neutralizing antibodies (non-NAbs) ...only broadly neutral- izing epitopes on the cell ...
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Mutations in Influenza A Virus Neuraminidase and Hemagglutinin Confer Resistance against a Broadly Neutralizing Hemagglutinin Stem Antibody
... FIG 2 Mutations inferred to be evolving under positive selection in the presence of the broadly neutralizing antibody F10. (A) Trajectories of select mutations elicited by viral passaging with F10, ...
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Dissecting the Neutralizing Antibody Specificities of Broadly Neutralizing Sera from Human Immunodeficiency Virus Type 1-Infected Donors
... elicit broadly neutralizing antibody responses by human immunodeficiency virus type 1 (HIV-1) vaccine antigens have been met with limited ...the neutralizing antibody specificities ...
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Yeast-Elicited Cross-Reactive Antibodies to HIV Env Glycans Efficiently Neutralize Virions Expressing Exclusively High-Mannose N-Linked Glycans
... The broadly neutralizing antibody 2G12, which binds a specific cluster of high-mannose glycans on HIV Env, shows that the glycan shield can also serve as a target for neutralizing ...and ...
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A Single Amino Acid Deletion in the Matrix Protein of Porcine Reproductive and Respiratory Syndrome Virus Confers Resistance to a Polyclonal Swine Antibody with Broadly Neutralizing Activity
... of broadly neutralizing antibody with a previously characterized linear epitope located between amino acids 43 and 51 in GP5 (16) (see ...of broadly neutralizing antibody could ...
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Anti-gp41 Antibodies Cloned from HIV-Infected Patients with Broadly Neutralizing Serologic Activity
... display antibody libraries that were constructed from patients and that were selected on recombinant gp41 showed a much lower frequency of cluster I binders (5 out of 25) ...However, antibody cloning by ...
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Antibody Response to Hypervariable Region 1 Interferes with Broadly Neutralizing Antibodies to Hepatitis C Virus
... HVR1 antibody inhibited the binding and neutralization of both sets of HC33 ...other broadly neutralizing HCV HMAbs that have contact residues outside aa 412 to ...of broadly neutral- izing ...
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Structure of a Classical Broadly Neutralizing Stem Antibody in Complex with a Pandemic H2 Influenza Virus Hemagglutinin
... To elucidate the molecular basis for C179 cross-reactivity, we determined the crystal structure of Fab C179 in complex with the HA from a pandemic H2N2 influenza virus [A/Japan/305/1957 (H2N2); Jap57/H2] at 2.90-Å ...
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The expanding array of HIV broadly neutralizing antibodies
... Simultaneous with the description of PGT151 addi- tional bnAbs were found to target the subunit interface region of vulnerability but each via distinctive Env con- tact sites. Notably, many were also identified by a neu- ...
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Characterization of a Broadly Neutralizing Monoclonal Antibody That Targets the Fusion Domain of Group 2 Influenza A Virus Hemagglutinin
... stalk-directed broadly neutralizing monoclonal antibodies (MAbs) that have been isolated and characterized to date bind and neutralize group 1 influenza A viruses ...first antibody described to have ...
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The Potent and Broadly Neutralizing Human Dengue Virus Specific Monoclonal Antibody 1C19 Reveals a Unique Cross Reactive Epitope on the bc Loop of Domain II of the Envelope Protein
... Temperature-dependent in vitro neutralization assays. Focus re- duction neutralization titer (FRNT) assays were performed with the dif- ferent DENV strains and MAbs on Vero cells. Purified MAbs were mixed with 100 ...
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Protective Capacity of the Human Anamnestic Antibody Response during Acute Dengue Virus Infection
... the antibody response, two smaller groups of antibodies bound to previously undescribed epitopes in domain II of the E ...anamnestic antibody response has a protective capacity despite moderate ...
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Mechanism of HIV-1 Neutralization by Antibodies Targeting a Membrane-Proximal Region of gp41
... are likely to neutralize by a common mechanism: targeting the fusion-intermediate state of gp41 with the help of their lipid bind- ing activity. The greater neutralization potency of 10E8 than of 4E10 highlights the ...
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