Brucella abortus
19. Seroprevalance of Brucella abortus in cattle and buffaloes in district Rajanpur, Punjab, Pakistan
... Pakistan. Brucella abortus was found ...cattle Brucella abortus percentage was much higher at various farms in Punjab, results indicated that ...[26]. Brucella abortus, ...
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Interactions between Brucella abortus and phagocytic leukocytes
... Several fractions isolated from Brucella abortus were examined for their ability to generate chemotactic factors from normal bovine serum for granulocytes and monocytes... Three differen[r] ...
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THE CHARACTERIZATION OF GENE ENCODING SURFACE PROTEIN-31(BCSP-31) Brucella abortus FIELD ISOLATE BY PCR TECHNIQUE
... Culture and reidentification of Brucella abortus field isolates wich had been previously described by Alton (1988). The samples are being cultured in the Brucella Medium Base (Oxoid cat no. CM0169) ...
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Characterization of Brucella abortus mutant strain Δ22915, a potential vaccine candidate
... against Brucella infection in livestock in developing countries and in wildlife in developed ...the Brucella abortus (B. abortus) S2308 mutant strain Δ22915, in which the putative lytic ...
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Evaluation of Indirect Enzyme-Linked Immunosorbent Assays and IgG Avidity Assays Using a Protein A-Peroxidase Conjugate for Serological Distinction between Brucella abortus S19-Vaccinated and -Infected Cows
... with Brucella infection (22, ...B. abortus-spe- cific IgG2 antibody levels may be a valuable tool in identifying Brucella-infected ...virulent Brucella strains when they are living in areas ...
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Characterization and Protective Property of Brucella abortus cydC and looP Mutants
... Brucella abortus readily multiplies in professional or nonprofessional phagocytes in vitro and is highly virulent in ...B. abortus biovar 1 strain IVKB9007 lacking the ATP/GDP-binding protein motif A ...
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Recent advances in Brucella abortus vaccines
... Brucella abortus S19 was isolated in 1923 from milk of a Jersey cow by Dr John Buck ...B. abortus S19 was proved by experimental tests in cattle [47,51] and under field conditions ...B. ...
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The CO2-dependence of Brucella ovis and Brucella abortus biovars is caused by defective carbonic anhydrases
... of Brucella ovis and most Brucella abortus biovars 1–4 ...reflects Brucella metabolism, an area of active ...B. abortus in rich media or ...of Brucella metabolism and, in the ...
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Immunochemical identification of Brucella abortus lipopolysaccharide epitopes.
... Protection against Brucella melitensis or Brucella abortus in mice with immunoglobulin G (IgG), IgA, and IgM monoclonal antibodies specific for a common epitope shared by the Brucella A [r] ...
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Brucella abortus: determination of survival times and evaluation of methods for detection in several matrices
... Background: Brucella abortus is a highly pathogenic zoonotic agent, tempting for the development of a rapid diagnostic method to enable adequate treatment and prevent further ...of Brucella spp ...
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A 6 Nucleotide Regulatory Motif within the AbcR Small RNAs of Brucella abortus Mediates Host Pathogen Interactions
... B. abortus strains produced the corresponding AbcR ...in Brucella abortus. Brucella abortus 2308, ΔabcR1, ΔabcR2, and ΔabcR1/2 strains were grown in brucella broth to an ...
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Optimization of Brucella abortus Protocols for Downstream Molecular Applications
... ABSTRACT We compared the performances of various DNA extraction kits for their ability to recover Brucella abortus strain 19 inoculated into Brucella-free bovine tis- sues. Tissues were homogenized ...
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ISOLATION AND PURIFICATION OF MAJOR OUTER MEMBRANE PROTEINS FROM BRUCELLA ABORTUS S-99
... Brucella abortus is an intracellular bacterium which resides in the macrophages of humans and animals where they are inaccessible to antibodies and complement ...
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Brucella abortus Infection of Placental Trophoblasts Triggers Endoplasmic Reticulum Stress Mediated Cell Death and Fetal Loss via Type IV Secretion System Dependent Activation of CHOP
... with Brucella abortus, the etiologic agent of brucellosis, the un- folded protein response in the infected placenta contributes to placentitis and abor- tion, leading to pathogen ...B. abortus ...
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Development of a Differential PCR Assay for Detection of Brucella abortus and Brucella melitensis: an Analytical Approach for Monitoring of Brucella spp. in Foods of Animal Origin
... genus Brucella. Among all Brucella species, B. melitensis, B. abortus, and ...of Brucella species is isolation of the bacteria on culture ...of Brucella bacteria is a time- and ...
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Cattle serologically positive for Brucella abortus have antibodies to B. abortus Cu-Zn superoxide dismutase.
... Lane 1, amido black-stained blot of BCSP; lane 2, immunoblot incubated with positive control serum from an experimen- tally infected cow (cow 47); lane 3, immunoblot with a negative cont[r] ...
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Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse
... In Brucella, many genes reportedly associated with carbon metabolism are necessary for virulence, such as 6-phosphogluco- nate dehydrogenase (gnd), phosphoglucose isomerase (pgi), pyruvate carboxylase (pyc) and ...
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Regulation of Brucella abortus catalase as a defensive mechanism against oxidative stress
... abortus is regulated at the transcriptional level in response to external hydrogen peroxide, and a catalase deletion mutant is more susceptible than wild type to external H2O2... 1 Prima[r] ...
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Conditions for conducting the indirect hemolysis test for detection of antibodies to Brucella abortus
... The possibility was considered that the amount of complement used for testing was limiting despite our intention to use excess amounts, because limitation of complement could result in d[r] ...
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Detection of serum antibody to Brucella abortus in cattle by use of a quantitative fluorometric immunoassay
... All four serological tests also showed a significantly higher P < 0.05 mean antibody titer in animals that were nonvaccinated and culture positive than in those that were nonvaccinated a[r] ...
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