CAS system
Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system
... immune system. The CRISPR/Cas system allows targeted cleavage of genomic DNA guided by a customizable small noncoding RNA, resulting in gene modifications by both non-homologous end joining (NHEJ) ...
10
An Attenuated CRISPR Cas System in Enterococcus faecalis Permits DNA Acquisition
... we introduced pheS* to allow for counterselection on para-chloro-phenylalanine (p-Cl- Phe) (36). The resulting plasmid was designated pGR-ermB (GenBank accession number MF948287) and has advantages over its parent ...
19
Cloning free CRISPR/Cas system facilitates functional cassette knock in in mice
... CRISPR/Cas system consisting of three components in- cluding Cas9, crRNA and tracrRNA (dual ...CRISPR/Cas system when combined with Cas9 ...CRISPR/Cas system, we tried to ...
11
Escherichia Coli CRISPR/CAS System Evolved with Bacteriophage: A Case Report
... In this case study, we demonstrated the results of sequence analysis which can help understanding the presence of CRISPR-me- diated anti-phage ability in pathogenic and non-pathogenic strains of E. coli . We collected E. ...
7
Cas6 specificity and CRISPR RNA loading in a complex CRISPR Cas system
... CRISPR-Cas system, and the ge- nomic CRISPR locus is protected against cleavage by effec- tor complexes due to detection of a perfect match between the genomic locus and the CRISPR sequence flanking the ...
10
Functional Characterization of CRISPR Cas System in the Ethanologenic Bacterium Zymomonas mobilis ZM4
... CRISPR-Cas system has been proved as an adaptive immune mechanism against mobile DNA elements like viruses and plasmids ...by Cas proteins to generate mature crRNA species ...the Cas proteins ...
12
<p>How CRISPR-Cas System Could Be Used to Combat Antimicrobial Resistance</p>
... CRISPR-Cas system with spacers that target genes of the Lambda phage, displayed decreased sensi- tivity to Lambda ...CRISPR-Cas system, which suggests a broader and more critical role for the ...
11
Advances in Engineering the Fly Genome with the CRISPR-Cas System
... premier system for understanding complex bi- ological processes at the molecular, cellular, and organismal ...(CRISPR)-Cas system, the researcher need only provide a small targeting RNA, which is ...
18
A New Group of Phage Anti CRISPR Genes Inhibits the Type I E CRISPR Cas System of Pseudomonas aeruginosa
... strain SMC4386. However, phage DMS3m also has an active type I-E anti-CRISPR gene (gene 30), encoding ACR3-30 (Fig. 3C), that is expected to protect it from inhibition by the CRISPR-Cas system. As shown in ...
7
Function of the CRISPR Cas System of the Human Pathogen Clostridium difficile
... CRISPR- Cas system is found in clostridia as well as in methanogenic and halophilic archaea and is defined by a subtype-specific protein ...I-B system in Cascade targeting to invader DNA has been ...
16
Mechanism of spacer integration links the CRISPR/Cas system to transposition as a form of mobile DNA
... (Cas) system, present in approximately 90% of archaea and approximately 50% of bacteria, is an adaptive immune system (reviewed in [1]) that generates small RNAs (known as crRNAs) transcribed from ...
5
Programmable RNA Shredding by the Type III-A CRISPR-Cas System of Streptococcus thermophilus
... the length of crRNA) and a 40-nt crRNA in the complex isolated via Csm2 (further termed Csm-40 complex). The MS analysis of the 72-nt crRNA is consistent with the pre-CRISPR cleavage at the base of the CRISPR RNA hairpin ...
67
CRISPR/Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery
... CRISPR/Cas system originates, it functions as an adaptive immune system that acts against bacteriophages and other invading nucleic acids ...CRISPR/Cas system found in Streptococcus ...
104
CRISPR-Cas Targeting and Escape in Lactobacillus gasseri.
... CRISPR-Cas system is provided with a self-targeting spacer sequence, CRISPR-Cas will destroy its own host’s ...CRISPR-Cas system. If the strain of interest contains a native ...
159
IoT based Facial Recognition Biometric Attendance
... A database of all the students will be created using python and open cv. It is a onetime process so that we will have a real time database to train our system and to match the captured faces. For creating a ...
8
On Solving Centrosymmetric Linear Systems
... linear system using Gauss or Gauss-Gordan algorithms is The motivation of the current paper is to develop efficient algorithms for solving any centrosymmetric linear system having equations and unknowns ...
12
Short and Long Term Hemodynamic and Clinical Effects of Carotid Artery Stenting
... after CAS, and evaluated the coherence of hemo- dynamic effects between 1 week and 1 year after CAS, empha- sizing that 1-week hemodynamic follow-up has great value for predicting long-term effects and can ...
7
Road safety study in New Zealand: Road Crashes involving overseas Drivers
... to CAS data provided by the New Zealand Transport Agency (NZTA), the percentage of the road crashes involving foreign drivers has gradually increased from ...
18
Clinical Use of a Computer Assisted Reverse Total Shoulder Arthroplasty System: an Analysis of 1702 Cases
... Navigated cases took significantly longer than non-navigated cases. Anecdotal individual surgeon time data suggests a learning curve, where the surgeon takes longer at first to get used to the system before ...
5
Thermodynamic Assessment of an Integrated Solar-Biomass System for Quadruple Generation Purposes
... polygeneration system based on solar and biomass to produce power, heating, hot water and ...thermal system in many power plants in the world because of their high efficiency and low cost ...
6