cDNA clones
Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae
... recombinant cDNA clones expressing antigens found in Onchocerca volvulus infective larvae is ...such clones, an expression cDNA library constructed from adult ...
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Recovery of Infectious Pariacoto Virus from cDNA Clones and Identification of Susceptible Cell Lines
... molecular clones, full-length cDNAs of PaV RNAs 1 and 2 were cotranscribed by T7 RNA poly- merase in baby hamster kidney cells that expressed T7 RNA ...infectious cDNA clones, cell culture ...
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Castor bean lectins : construction and sequencing of cDNA clones
... Small-scale plasmid preparations were performed on all the colonies which hybridised with the oligomer, and their sizes were crudely estimated by comparison with those of supercoiled pBR322 and pAT153. The eight largest ...
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Isolation of cDNA clones for genes that are expressed in the tail region of the ascidian tailbud embryo
... Int J U "lIiol ~I h91 h98 119971 691 {)ri~ill(/I Arlirll' Isolation of cDNA clones for genes that are expressed in the tail region of the ascidian tailbud embryo HIROKI TAKAHASHI', KOUICHIISHIDA, KAZU[.] ...
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Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones
... high-throughput cDNA sequencing projects (Ota et ...of clones, the number of libraries, major tissue origins, methods, and URLs of cDNA clones for each cDNA project are summarized in ...
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Construction of rubella virus genome-length cDNA clones and synthesis of infectious RNA transcripts.
... In the communication in which the sequence of the RUB genomic RNA was reported 7, dideoxy sequencing using polyA+ RNA extracted from RUB-infected cells as a template and oligonucleotide [r] ...
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Infectious cDNA clones of the DA strain of Theiler's murine encephalomyelitis virus.
... Construction of pDAFL3, an infectious cDNA clone DA strain in a transcription vector, in which pDAFL1 was reengineered so that the 5' end of the DA genome was placed two nucleotides down[r] ...
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Development of infectious cDNA clones of Salmonid alphavirus subtype 3
... Recovery of infectious recombinant SAV from cDNA The ability of the full-length clone to express viral structural proteins was addressed using the obtained antiserum against E2. IFAT with anti-E2 on trans- fected ...
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In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14.
... Previous studies with poliovirus 11, 13, 14 have demonstrated that a full-length cDNA clone, representing the entire genome RNA, could be used to generate progeny virus upon transfection[r] ...
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Unusual, stable replicating viruses generated from mumps virus cDNA clones
... Plasmids that expressed the N, P and L proteins with the authentic sequence of the MuV G09 virus were generated to act as helper plasmids in the rescue experiments. Plasmids were also generated that represented the full ...
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Temporal regulation of baculovirus RNA: overlapping early and late transcripts.
... To investigate the mechanisms involved in the regulation of AcNPV gene expression, we have hybridized recombinant clones of small genomic restriction fragments or cDNA clones of viral mR[r] ...
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Characterization of a Marek's disease virus BamHI-L-specific cDNA clone obtained from a Marek's disease lymphoblastoid cell line.
... 1191 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Two Marek's disease MD virus BamHI-L-specific cDNA clones were isolated from a cDNA library constructed from polyA+ [r] ...
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Hepatitis E virus: identification of type-common epitopes.
... The two cDNA clones, 406.4-2M and 406.3-2M, were identified directly from human stool by using human sera and therefore represent not only the initial isolation of HEVM strain clones but[r] ...
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Detection of candidal antigens in autoimmune polyglandular syndrome type I.
... candidal cDNA expression library with patient sera and found four cDNA clones encoding the immunopositive proteins enolase, heat shock protein 90, pyruvate kinase, and alcohol ...
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Molecular cloning and characterization of human cardiac alpha and beta form myosin heavy chain complementary DNA clones Regulation of expression during development and pressure overload in human atrium
... (MHC) cDNA clones (pHMHC2, pHMHC5) from a fetal human heart cDNA ...these cDNA clones, we demonstrate that pHMHC2 is exclusively transcribed in the atrium, whereas the mRNA for pHMHC5 ...
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Isolation of a complementary DNA clone for the human complement protein C2 and its use in the identification of a restriction fragment length polymorphism
... (cDNA) clones corresponding to the major histocompatibility (MHC) class III antigen, complement protein C2, have been isolated from human liver cDNA libraries with the use of a complex mixture of ...
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Characterization, cloning and immunogenicity of antigens released by lung-stage larvae of Schistosoma mansoni
... Clone NLSL19 was found to have significant sequence homology to the S5a subunit of the 26S proteasome of different species. The 26S proteasome is composed of 2 subcomplexes, the 20S proteasome (or multicatalytic ...
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EVALUATING THE OUTCOME OF TRANSFORMING GENETICALLY MODIFIED E COLI CELLS WITH HUMAN COAGULANT FACTOR IX CDNA
... creating cDNA library with efficient amplification of the gene of ...of cDNA clones before amplification to be more specific when optimising PCR ...desired cDNA is present and to ascertain the ...
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A CDNA selection approach to isolate Y-linked genes expressed in testis
... the cDNA clones m llS g l and m l2 2 h l0 are positioned in three overlapping genomic fragm ents AC006335, ACO 10891 and A C017019 that m ake up this ...TTY cDNA has been m apped w ithin the AZFc ...
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Autonomous expression of RTVL-H endogenous retroviruslike elements in human cells.
... Characterization of cDNA clones corresponding to transcripts present in NTera2Dl cells indicates that the complex transcription pattern observed in these cells is generated by the follow[r] ...
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