cDNA library
Generation and analysis of expressed sequence tags from a cDNA library of the fruiting body of Ganoderma lucidum
... this cDNA library were analyzed for similarities by perform- ing BLAST searches against public databases, including SwissProt [29], Nr [30] and Nt ...
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Construction and analysis of cotton (Gossypium arboreumL ) drought related cDNA library
... subtractive cDNA library containing water-stress induced transcripts in cotton seed- ...SSH cDNA library contained many genes related to drought- stress ...
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Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger
... unamplified library was ...the cDNA library attained to the requirements of a standard cDNA ...This library provided a useful platform for the functional genomic research of Siberian ...
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An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L
... acids [12-14]. During maturity, large amounts of fatty acids/lipids, several toxic compounds and other second- ary metabolites are found in jatropha seeds particularly in four to five-week-old seeds [15]. These compounds ...
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Rauber, Lucio (2008): Qualitative and quantitative analysis of porcine sperm transcripts and characterization of a normalized cDNA library. Dissertation, LMU München: Tierärztliche Fakultät
... a cDNA library and a microarray were ...normalized cDNA library the total RNA was extracted and first strand cDNA synthesis was carried out with an oligo(dT) ...resulting cDNA ...
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Characterization of an Atlantic cod (Gadus morhua) embryonic stem cell cDNA library
... importance for ES cells to maintain their pluripotency, explaining the high abundance of chromatin-related tran- scripts in the examined ES cell cDNA library. Also among the most abundant transcripts were ...
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Effective Procedure for Development of EST SSR Markers Using cDNA Library
... and 8.5 μl nuclear free water were mixed to give a final volume of 50 μl, after which PCR was performed by sub- jecting the samples tothe following conditions: 2 minutes at 94˚C followed, by 35 cycles of 30 seconds at ...
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Screening for plant transporter function by expressing a normalized Arabidopsis full-length cDNA library in Xenopus oocytes
... the library have no known ...the library and their corresponding predicted functions is included as supplementary information [see Additional file ...transporter cDNA library for glucose ...
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Functional cDNA library for efficient expression of measles virus-specific gene products in primate cells.
... COS cells infected with measles virus produced two species of P-related proteins, P and P2, which were immunoprecipitated by anti-measles virus serum as well as by monoclonal antiserum a[r] ...
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A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library
... of cDNA should be input into the PCR amplification step and (iii) PCR cycles should be optimised so that the amplification reaction is stopped whilst still in the linear phase (27–29 ...
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Characterization of a Marek's disease virus BamHI-L-specific cDNA clone obtained from a Marek's disease lymphoblastoid cell line.
... 1191 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Two Marek's disease MD virus BamHI-L-specific cDNA clones were isolated from a cDNA library constructed from polyA+ [r] ...
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Identification of immune protective genes of Eimeria maxima through cDNA expression library screening
... The cDNA entry library was constructed using Clone- Miner™ II cDNA Library Construction Kit (Invitrogen) following manufacturer’s protocol (see Additional file 1: Protocol for constructing ...
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Cytoplasmic leucyl-tRNA synthetase of Neurospora crassa is not specified by the leu-5 locus.
... We generated a Xgtl 1 Neurospora crassa cDNA library and screened the library for the cytoplasmic leucyl-tRNA synthetase (cyto LeuRS) clones using cyto LeuRS specific antib[r] ...
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Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line
... BIN-67 cDNA library was screened using a cDNA probe from a porcine renal CTR (pCTR) that we recently ...this cDNA into COS cells resulted in expression of receptors with high affinity for ...
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A second superoxide dismutase gene in the medfly, Ceratitis capitata.
... was isolated from a medfly third-instar cDNA library, using as a probe a PCR fragment amplified from adult. cDNA[r] ...
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ANTIBODY TO MITOCHONDRIAL COMPLEX-I IN SOME PATIENTS WITH MULTIPLE SCLEROSIS
... To locate new autoantigens in MS, a control pool of 5 samples ofigG from patients with MS was thoroughly adsorbed with the E. coli host (YI 090) which was used for expression of proteins in a AgII cDNA ...
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Structural and Immunological Characteristics of a 28-Kilodalton Cruzipain-Like Cysteine Protease of Paragonimus westermani Expressed in the Definitive Host Stage
... complete cDNA sequence encoding a 28-kDa cruzipain-like cysteine protease of adult Paragonimus wester- mani, termed Pw28CCP, was isolated from an adult cDNA ...The cDNA contained a single open ...
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Molecular genetics of sulphate assimilation in 'Arabidopsis thaliana'
... Functional complementation of the Escherichia coli cysteine auxotrophic strain JM15 serine acetyltransferase deficient using an Arabidopsis thaliana cDNA library in the expression.. memb[r] ...
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Identification and Characterization of a Differentially Expressed Gene (07E12) in the Infective Larvae of the Parasitic Nematode Ascaris suum.
... It has been suggested that neuropeptides in nematodes play widespread and varied roles in the nervous system via cellular expression pat- terns and genetic analysis. Because the ana- tomical comparison of C. elegans with ...
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Molecular cloning and characterization of pigeon (Columba liva) ubiquitin and ubiquitin-conjugating enzyme genes from pituitary gland library
... full-length cDNA li- brary was constructed using the pituitary gland of white king pigeon with SMART LD-PCR techniques ...the cDNA sequence revealed that the protein is ubiquitin, a highly conserved protein ...
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