cDNA library screening and plaque purification
Increasing the library size in cDNA display by optimizing purification procedures
... For nearly two decades, evolutionary molecular engin- eering (directed evolution) has played a role as a com- plementary partner to rational protein design in protein engineering [1]. In evolutionary molecular ...
5
Identification of immune protective genes of Eimeria maxima through cDNA expression library screening
... The effective antigens also could be masked by the dilu- tion effect during cDELI. For example, if one sub-library contains 100 plasmids during cDELI, each individual plasmid will be delivered in 1/100th of the ...
10
Purification and cDNA sequencing of C4l: An actin-associated protein
... in screening has been successful, there is considerable uncertainty in the hybridisation conditions because the binding of the oligonucleotides depends on the length of the hybrid formed and the ...when ...
267
Screening lambda cdna or genomic libraries
... for cDNA library, ~10 hrs for a genomic ...the library to be plated for screening at a density such that the plaques can grow to about 1 mm in diameter before they begin to just barely touch ...
10
Screening for plant transporter function by expressing a normalized Arabidopsis full-length cDNA library in Xenopus oocytes
... a library which is less likely to contain possible deleterious plant genes that might affect oocyte ...the library with full-length transporter cDNAs as they become released, and eventually to saturate the ...
9
Identification of stroke associated antigens via screening of recombinant proteins from the human expression cDNA library (SEREX)
... Toshio Machida 1 , Motoo Kubota 2 , Eiichi Kobayashi 3 , Yasuo Iwadate 3 , Naokatsu Saeki 3 , Akira Yamaura 4 , Fumio Nomura 5 , Masaki Takiguchi 6 and Takaki Hiwasa 6* Abstract Background: Because circulating antibodies ...
11
Screening of an endothelial cDNA library identifies the C terminal region of Nedd5 as a novel autoantigen in systemic lupus erythematosus with psychiatric manifestations
... endothelial cDNA expression library and we screened it with a serum with an ele- vated AECA titer, we found no significant correlation between the presence of AECAs and the presence of anti-Nedd5 anti- ...
8
Construction and analysis of cotton (Gossypium arboreumL ) drought related cDNA library
... subtracted cDNA library is obtained, it is impor- tant to confirm that individual clones indeed represent differentially expressed ...tial screening of the subtracted library helps to ...
8
Effective Procedure for Development of EST SSR Markers Using cDNA Library
... 2.4. cDNA Library Transformation to Vector The cDNA librarywas purified using a PCR purification kit ...and cDNA PCR products were adjusted to the ...
6
Yeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
... Arabidopsis cDNA library insertion, but only 35 colonies out of that were able to show blue staining in X-gal ...of cDNA expression it might be concluded that a few proteins have a potential for true ...
6
Liu, Zhijie (2010): Identification of Novel Genes for the Development of a Rapid Diagnostic Test for Theileria uilenbergi Infection by Screening of a Merozoite cDNA Library. Dissertation, LMU München: Tierärztliche Fakultät
... the cDNA library led to the discovery of 12 single clones, among which Clone 2, 9 and 26 exhibited a high degree of identity, especially at the 3' terminus and 3' untranslated region, indicating that they ...
81
Purification and cDNA cloning of the ovigerous hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir
... doi:10.1242/jeb.00785 The egg attachment system of an estuarine crab Sesarma haematocheir is formed on the maternal ovigerous hairs just after egg laying, and slips off these hairs just after hatching. The stripping is ...
12
An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L
... RNA purification and cDNA ...RNA purification, cDNA synthesis, cDNA library construction, RT-PCR, and Northern ...the cDNA library seems to be a good source of ...
6
Optimization and comparison of different methods for RNA isolation for cDNA library construction from the reindeer lichen Cladonia rangiferina
... After purification with the RNeasy clean- up kit, which includes a DNase I treatment, the DNA con- tamination was no longer visible following denaturing gel electrophoresis (figure 1), but there was a marked ...
5
Soliman, Hatem Mohamed Touhan (2005): Construction and Screening of an Expression cDNA Library from the Triactinomyxon Spores of Myxobolus cerebralis, the causative agent of Salmonid Whirling Diseases. Dissertation, LMU München: Tierärztliche Fakultät
... construction, cDNA libraries are typically amplified to provide a near limitless source of cDNA clones, this was not done for the triactinomyxon cDNA library in order to preserve accurate ...
89
The effect of column purification on cDNA indirect labelling for microarrays
... the cDNA and oligonucleotide microarrays, Cy3 and Cy5 are commonly used fluorescent dyes that are exited by different wavelengths of ...labeled cDNA samples and hybridizing to a microarray ...successful ...
8
2 was GCGCGCAAGCTTCCNGTNGGNGAYGAYT- screen a HeLa cdna library for isolation of the full-length
... Reconstitution of p53-ubiquitinylation reactions from purified components: The role of human ubiquitin-conjugating enzyme UBC4 and E6-associated protein (E6AP).. (human papilloma virus/c[r] ...
5
Screening for candidate genes related to breast cancer with cDNA microarray analysis
... occurrence as well as development of malignant diseases. CXCR4, a 352-amino acid rhodopsin-like G protein- coupled receptor (GPCR), which can selectively bind to the CXC chemokine stromal cell-derived factor 1 (SDF-1), ...
8
Characterization of an Atlantic cod (Gadus morhua) embryonic stem cell cDNA library
... post-blastula transcriptional drop. In addition, the detec- tion of another ES cell specific gene, an ortholog of the mammalian Pou5f1, validated the authenticity of the blastula-stage cDNA library. Two ...
8
Protocol v001 Page 1 of 1 AGENCOURT RNACLEAN XP IN VITRO PRODUCED RNA AND CDNA PURIFICATION
... Calculation of Percent Recovery: To gauge percent recovery of RNA or cDNA, analyses of the samples pre-purification and post- purification are necessary. For RNA, Agencourt recommends either a ...
14