cecal ligation and puncture
Prognosis of sepsis induced by cecal ligation and puncture in mice improved by anti Clonorchis Sinensis cyclopholin a antibodies
... Methods: The mice model with sepsis was established with cecal ligation and puncture (CLP). Fifty mg/kg purified anti-CsCyPA antibodies were injected via the caudal vein 6 h after the CLP operation, ...
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Endothelial dysfunction is a potential contributor to multiple organ failure and mortality in aged mice subjected to septic shock: preclinical studies in a murine model of cecal ligation and puncture
... of cecal ligation and puncture-induced septic shock, whether aging increases the severity of endo- thelial dysfunction, leading to a worsening of oxidative stress, tissue inflammation and, ...
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Body temperature and mouse scoring systems as surrogate markers of death in cecal ligation and puncture sepsis
... Experimental animal models have been created and refined for over 80 years to investigate the development, management, and treatment of sepsis [1], and have contributed to many important advances in our understanding of ...
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Sepsis modeling in mice: ligation length is a major severity factor in cecal ligation and puncture
... for cecal puncture; the number of punctures, generally between 1 and 4; and the use of antibiotics and/or fluid resuscitation [10, ...of cecal ligation is often not described in CLP animal ...
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Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats
... This study describes the acute kidney injury and inflamma- tory response of sepsis following cecal ligation and puncture in d -gal-induced aging rats. The results showed that among the three groups ...
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Kinetics and protective role of autophagy in a mouse cecal ligation and puncture induced sepsis
... Figure 2 Co-localization of green fluorescent protein (GFP)-Microtubule-associated protein light chain 3 (LC3) dots with lysosome- associated membrane protein type 1 (LAMP1) in the liver after cecal ...
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Western diet enhances hepatic inflammation in mice exposed to cecal ligation and puncture
... to cecal ligation and puncture (CLP) to induce sepsis as described previously ...third). Cecal contents were manually extruded and spread with a sterile cotton ...without ligation and ...
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Pattern of cytokines and pharmacomodulation in sepsis induced by cecal ligation and puncture compared with that induced by endotoxin.
... The production of tumor necrosis factor alpha (TNF- a ), interleukin-1 b (IL-1 b ), and IL-6 and their phar- macomodulation were evaluated in a model of polymicrobial sepsis induced in mice by cecal ...
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Use of Tat-Hsp70 Fusion Protein to Attenuate Sepsis-Induced Lung Injury in Rodents With Acute Respiratory Distress Syndrome Caused by Cecal Ligation and Double Puncture (2clp)
... Background: Sepsis, a fatal syndrome of dysregulated inflammation, is the leading cause of death in the critically ill. The lung is the organ most often injured, developing into Acute Respiratory Distress Syndrome ...
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Effects of crystalloids and colloids on liver and intestine microcirculation and function in cecal ligation and puncture induced septic rodents
... For example, in the Scandinavian Starch for Severe Sepsis/Septic Shock (6S) trial, patients in the HES group received almost the same amount of volume when compared with patients in the [r] ...
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Original Article Protective effects of curcumin on hepatocytes in cecal ligation and puncture-induced sepsis in rats
... Sprague-Dawley rats were randomly divided into six groups of 20 rats each: animals under- going sham CLP Sham group, animals under- going CLP CLP group, animals undergoing CLP and treate[r] ...
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Changes in ceftriaxone pharmacokinetics/pharmacodynamics during the early phase of sepsis: a prospective, experimental study in the rat
... The SD rats were initially housed three per cage in a temperature (18–22 °C) and humidity-controlled room under a constant 12-h light/dark cycle and fed with standard rat chow and water ad libitum. After acclima- ...
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Role of the IL-33-ST2 axis in sepsis
... CLP: Cecal ligation and puncture; DAMPs: Danger-associated molecular patterns; DCs: Dendritic cells; GMC- SF: Granulocyte macrophage colony-stimulating factor; GRK2: G protein-coupled re- ceptor ...
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Adenoviral transfer of HSP 70 into pulmonary epithelium ameliorates experimental acute respiratory distress syndrome
... in cecal ligation and double puncture (2CLP), looking at liver and lung, have demonstrated an alteration in protein expression that suggests a potentially useful therapeutic approach ...after ...
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Galectin 9 prolongs the survival of septic mice by expanding tim 3 expressing natural killer T cells and PDCA 1+ CD11c+macrophages
... Figure 5 Effects of galectin (Gal)-9 on subpopulations of spleen T cells. Spleen cells were obtained from PBS- or Gal-9-treated cecal ligation and puncture (CLP) mice at 24 hours after a single ...
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Increased plasma levels of endozepines, endogenous ligands of benzodiazepine receptors, during systemic inflammation: a prospective observational study
... Sepsis was induced by cecal ligation and puncture (CLP) as previously described [18]. Rats were anesthetized with ketamine (80 mg/kg) and xylazine (10 mg/kg). A 2-cm midline incision was made; the ...
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The role of CXCL10 in the pathogenesis of experimental septic shock
... less systemic cytokine production, attenuated physiologic dysfunction and improved survival during severe sepsis caused by cecal ligation and puncture (CLP) [11]. Large numbers of CXCR3 + NK cells ...
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Receptor interacting protein kinase 3 deficiency inhibits immune cell infiltration and attenuates organ injury in sepsis
... Figure 2 Ripk3 −/− mice have attenuated lung damage after CLP. The lung tissues from WT and Ripk3 −/− mice were harvested 20 hours after sham- or CLP-operation. (A) Representative lung histology sections (H & E ...
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Granulocyte and monocyte adsorptive apheresis ameliorates sepsis in rats
... Conclusions: This study showed that selective granulocyte and monocyte adsorption with cellulose acetate beads might ameliorate cecal ligation and puncture (CLP)-induced sepsis and impro[r] ...
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Critical illness induces nutrient independent adipogenesis and accumulation of alternatively activated tissue macrophages
... a cecal ligation and puncture (CLP)-fed mouse (B), Proliferating Cell Nuclear Antigen (PCNA) gene expression (C), percentage stained area for peroxisome proliferator-activated receptor g (PPARg) (D), ...
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