Cell-based high-throughput screening (HTS) assay
Development of a robust cell-based high-throughput screening assay to identify targets of HIV-1 viral protein R dimerization
... Imaging-based high content screening using automated fluorescence microscopy has several advantages over other screening ...a high content screen is the ...a cell-based ...
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Microfluidic Technologies for High-Throughput Screening Applications
... , screening such libraries for other properties such as catalytic activity has remained a ...The screening of a large cell population of the basis of biocatalytic activity primarily involves the ...
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Cell-based high-throughput screens for the discovery of chemotherapeutic agents
... human cell lines [27-30] led to enormous efforts beginning in the 1980s to generate inhibitors that could be used to reverse ...MDR cell lines to aid in the discovery of novel inhibitors of P-gp and other ...
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Assay development and high-throughput screening for small molecule inhibitors of a <em>Vibrio cholerae</em> stress response pathway
... In this study, we used a cell-based, HTS to target a con- served Gram-negative stress response pathway. Although we ultimately identified seven compounds that validated the expected phenotype after purchase ...
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A luciferase based viability assay for ATP detection in 384 well format for high throughput whole cell screening of Trypanosoma brucei brucei bloodstream form strain 427
... whole cell screening for HAT. A viability assay which has been extensively reported in the literature for all ...Blue assay for T.b.brucei whole cell viability esti- mation ...Blue ...
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High throughput screening in niche based assay identifies compounds to target preleukemic stem cells
... T-ALL cell lines, Danièle Gagné and Gael Dulude for assistance with flow cytometry, Valérie Villeneuve for sequencing, and Jean Duchaine for HTS and for the chemical ...
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Industrial scale high throughput screening delivers multiple fast acting macrofilaricides
... scale screening has delivered an enhanced portfolio of anti-Wolbachia chemotypes and identified 9 distinct chemical entities that represent excellent starting points for the discovery of novel drugs for the ...
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A high-throughput cellulase screening system based on droplet microfluidics
... the screening process. Classical screening uses microtiter plates (MTPs), where each well contains cells expressing a single type of mutant ...of screening is the bottle- neck in directed evolution, ...
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A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells
... Stable cell lines used in the TANGO and PRESTO -TANGO assays are generated by traditional transfection ...a cell line due to low efficiency and multiple rounds of transfection and antibiotic selection is ...
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High-content, high-throughput screening for the identification of cytotoxic compounds based on cell morphology and cell proliferation markers.
... novel high-content image analysis algorithm for use in primary screening assays to identify compounds with adverse cellular effects, by detecting abnormal- ities in cell morphology and mitotic ...
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A novel cell-free mitochondrial fusion assay amenable for high-throughput screenings of fusion modulators
... automated assay design to quan- tify mammalian mitochondrial fusion in vitro ...this assay perfectly suited for screening factors required for ...this assay are that we do not differentiate ...
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A cell based high throughput screening approach for the discovery of new inhibitors of respiratory syncytial virus
... To begin to probe the mechanism of action of the sulfonylpyrrolidines, the window of inhibitory activity in the cell-based assay was refined. Potency of compounds over time following infection was ...
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Robust High Throughput Real time Monitoring Assay for the Specific Screening of Bacterial Cell Envelope Inhibitors
... bacterial cell viability by classical microbiological methods include time consuming and labour intensive techniques such as growth assay by monitoring OD 600 and counting of colonies on the agar plate in ...
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A High-Throughput Assay for Screening of Natural Products that Enhanced Tumoricidal Activity of NK Cells
... NK cell-mediated killing of NSCLC cells, and this enhancement was ...NK cell immuno- therapy, and that the IFN-γ based high-throughput screening assay can be a reliable ...
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Identification of vitamin B1 metabolism as a tumor-specific radiosensitizing pathway using a high-throughput colony formation screen
... standard assay for determining reproductive cell death after radiation treatment, since effects on proliferation often do not reflect ...a high-throughput radiosensitivity screening ...
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A high throughput cell migration assay using scratch wound healing, a comparison of image based readout methods
... the assay by visual ...the assay in high-throughput format. However, screening large numbers of perturbations by vis- ual inspection is limited by subjectivity, operator fatigue, and ...
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Development of a high throughput cell based 384 well influenza A quantification assay for interpandemic and highly pathogenic avian strains
... a high-throughput format. Currently, several high throu- ghput assays to detect influenza virus exist which utilize reverse transcription polymerase chain reaction (RT-PCR) alone [3], and coupled ...
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R4 and R12 subfamily RGS proteins : structures, functions, and emerging chemical biology
... chips (Biacore) to densities of ~6000 RU as previously described [14]. In pilot studies, CCG- 4986 was observed to react with, and thereby irreversibly modify, the biosensor surface, preventing us from obtaining ...
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A Novel, Sensitive Assay for High Throughput Molecular Detection of Plasmodia for Active Screening of Malaria for Elimination
... hybridization assay. To develop an assay that can be used as an initial screening tool for malaria, we designed a set of 22 oligonucleotide probes (probe sequences available upon request) accord- ing ...
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Verdorfer, Tobias (2018): Multiplexed single-molecule force spectroscopy and activity studies on cellulosomes. Dissertation, LMU München: Fakultät für Physik
... assembly of molecular devices with designed properties. From a technical point of view, we were able to measure seven constructs using a single cantilever in two separate experiments by utilizing a fast and parallelized ...
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