cell-cell fusion
The hallmarks of cell cell fusion
... for cell-cell fusion in ...hypodermal fusion (top) in an elongating ...epithelial fusion failure phenotype in an eff-1 null mutant demonstrates that EFF-1 is required for ...
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Previously Unrecognized Amino Acid Substitutions in the Hemagglutinin and Fusion Proteins of Measles Virus Modulate Cell-Cell Fusion, Hemadsorption, Virus Growth, and Penetration Rate
... small-cell-cell fusion, by using GeneJuice (Novagen, Gibbstown, NJ) and Lipofectamine 2000 reagent (Invitrogen), respec- ...of fusion-inhibiting peptide (FIP; Z- D -Phe-Phe-Gly) (Peptide ...
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Detection of Cell-Cell Fusion Mediated by Ebola Virus Glycoproteins
... of cell-cell fusion mediated by a filovirus GP is unprecedented to our ...the fusion process, as well as issues related to the cytotoxicity of these viral proteins or to the predominant ex- ...
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Characterization of Human Cytomegalovirus Glycoprotein-Induced Cell-Cell Fusion
... Cell-cell fusion assays have been used as a tool to identify the fusion machinery of several other ...inducing cell-cell fusion ...herpesvirus fusion machines, it ...
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Amphotericin B derivative blocks human immunodeficiency virus type 1 entry after CD4 binding: effect on virus-cell fusion but not on cell-cell fusion.
... and cell-cell fusion had the same mechanism, drugs blocking HIV-1 entry but not syncytium formation, such as bicyclams (10) or hyperi- cin (11, 17, 20), have been proposed to act on a postfusion step ...
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Induction of cell cell fusion by ectromelia virus is not inhibited by its fusion inhibitory complex
... lent to >100pfu/cell) at neutral pH. In both conditions, already at 2 hpi the cultured cells were fused and syncytia dominated the entire culture exhibiting giant polykaryo- cytes consisting tens to hundreds ...
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Antibodies to CD9, a Tetraspan Transmembrane Protein, Inhibit Canine Distemper Virus-Induced Cell-Cell Fusion but Not Virus-Cell Fusion
... Interesting cell surface proteins which play a role in cell-cell fusion induced by viruses have been identified as fusion regu- lation protein 1 (FRP-1) and ...duced ...
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Antigenic Properties of the Human Immunodeficiency Virus Envelope during Cell-Cell Fusion
... fusing cell interface indicates that neutralizing humoral responses to the coreceptor-binding site may be extremely difficult, if not impossible, to ...of cell-cell ...of cell-cell ...
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Induction of Cell-Cell Fusion from Without by Human Herpesvirus 6B
... C). Fusion was detectable in both cell lines at 2 hpi, and widespread cell-cell fusion was observed at 4 hpi ...The fusion between cells that formed contact occurred within an ...
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Localization of a Region in the Fusion Protein of Avian Metapneumovirus That Modulates Cell-Cell Fusion
... to cell surface receptors triggers Paramyxovirinae F proteins, although the detailed molecular mechanism underlying fusion triggering is poorly ...the fusion of hMPV F protein through protonation of ...
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Membrane Fusion-Mediated Autophagy Induction Enhances Morbillivirus Cell-to-Cell Spread
... or cell-cell fu- ...similar cell-associated virus titers obtained in con- trol and Atg7-knockdown ...reduced cell-cell fusion and spread of the virus from the initially infected ...
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Monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein.
... The antiVP 70 antibody neutralized virus without complement and inhibited cell-cell fusion of previously infected HEp-2 cells, thus identifying VP 70 as the fusion protein.. such as a he[r] ...
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Attachment of Sendai virus particles to cell membranes: dissociation of adsorbed virus particles with dithiothreitol.
... When Sendai virus SV particles are incubated with human erythrocytes HE, they cause agglutination at 4°C virus-cell binding and induce hemolysis virus-cell fusion and cell-cell fusion at[r] ...
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Epstein-Barr Virus Fusion with Epithelial Cells Triggered by gB Is Restricted by a gL Glycosylation Site
... protein-based cell-cell fusion assay by monitoring reassembly of Renilla luminescence versus GFP fluorescence in a time-dependent ...core fusion machinery composed of gB and gH/gL ...epithelial ...
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Hydrophobic α-Helices 1 and 2 of Herpes Simplex Virus gH Interact with Lipids, and Their Mimetic Peptides Enhance Virus Infection and Fusion
... the cell differs from that of well-known viruses, in that it requires a multicomponent fusion ...multicomponent fusion machinery, enters target cells by fusion of the envelope with either the ...
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Maintenance of Mating Cell Integrity Requires the Adhesin Fig2p
... Mating cell morpho- genesis and zygotic spindle alignment can be rescued by ele- vated osmolarity, while cell-cell fusion, agglutination control, and cell integrity differences in fig2⌬ ...
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Events in the endoplasmic reticulum abrogate the temperature sensitivity of Sindbis virus mutant ts23.
... Temperature-sensitive mutations in proteins produced at or heated to a nonpermissive temperature render the proteins defective in some aspect of their maturation into functional entities. The characterization of ...
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Reovirus FAST Protein Transmembrane Domains Function in a Modular, Primary Sequence-Independent Manner To Mediate Cell-Cell Membrane Fusion
... virus fusion proteins in the transition from hemifusion to stable pore formation (29, 30, 35, ...hemi- fusion mutants of the FAST proteins, using the diversity of approaches applied to analyze hemifusion ...
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Cytotrophoblast stem cell lines derived from human embryonic stem cells and their capacity to mimic invasive implantation events
... Our cell lines continued prolifera- tive capacity without HESCs or feeder cells, strongly indicating the presence of a subpopulation of ...the cell line to revert to HESCs or pluripotency (other than ...
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Antiviral properties of two trimeric recombinant gp41 proteins
... In this study, we present the design of two soluble gp41- derived trimeric recombinant proteins produced in E. coli, which were termed Rgp41A and Rgp41B. Each of these two proteins are constituted of an N-domain spanning ...
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