Cell-culture
Identification of molecular determinants of cell culture growth characteristics of Enterovirus 71
... In addition, we genetically modified the EV-A71 sub- genotype B4 virus by substituting a single nucleotide at either position 475, 486, or 487 based on the fact that the corresponding nucleotides are responsible for the ...
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Mammary epithelial cell transformation: insights from cell culture and mouse models
... in cell culture by serial introduction of SV40 large T and small t, hTERT, and activated Ras ...in cell culture scheme suggests that perturbation of p53, pRb, protein phosphate 2A, telomerase, ...
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Setting up a Cell Culture Laboratory
... the culture output of several ...flask culture vessels may also require special incubators or ...General cell culture incubators require the ambient temperature to be usually 5–10°C colder ...
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Influence of cell type and cell culture media on the propagation of foot and mouth disease virus with regard to vaccine quality
... suspension cell culture to expand the virus to large ...both cell culture systems ...serum-containing cell culture media, need special ...
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Integrated Microfluidic Devices for Cell Culture and Assay
... the cell culture level with low shear ...of cell (10 µm) is around ...other cell types to further simulate the various characteristics of the in vivo cellular ...As cell- based assays ...
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Replication of West Nile virus, Rabensburg lineage in mammalian cells is restricted by temperature
... avian cell cultures, house sparrows or chickens, but the virus efficiently infected mosquito cells [14], ...mosquito cell cul- ture and reports of RABV replicating on vertebrate cell culture ...
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Effect of Faecalibacterium prausnitzii on intestinal barrier function and immune homeostasis : a dissertation presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Nutritional Science, Massey University, Manawatū, New Zealand
... For their technical assistance, I would like to thank Kelly Armstrong, Leigh Ryan and Stacey Burton, for always taking the time to answer my questions and helping out with the cell culture in busy times. ...
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Purification of Dengue Virus Particles by One Step Ceramic Hydroxyapatite Chromatography
... of culture fluid from dengue virus type 2 were loaded onto the column with 10 mM NaPB pH ...the culture medium and the cell culture fluid without virus were also loaded onto the ...
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Diagnosis of Tick Borne Encephalitis by a μ Capture Immunoglobulin M Enzyme Immunoassay Based on Secreted Recombinant Antigen Produced in Insect Cells
... concentrated cell culture superna- tant by five different monoclonal antibodies directed against TBEV E protein and in diluted (1:5) cell culture supernatant by 48 TBEV IgM-positive human ...
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Cell culture adaptation of Puumala hantavirus changes the infectivity for its natural reservoir, Clethrionomys glareolus, and leads to accumulation of mutants with altered genomic RNA S segment.
... the cell culture experiments, in which the E6 strains replicated to a much higher ...the cell culture adaptation process varies from one trial to another, although the same inoculum was used: ...
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INFLUENCE OF SOME PRECURSORS SUPPLEMENT ON GROWTH AND PRODCUTION OF ALPHATOCOPHEROL AND PIGMENTS IN CALLUS AND CELL SUSPENSION CULTURES OF CARTHAMUS TINCTORIUS L
... and cell suspension cultures. The callus and cell suspension growth and alphatocopherol and pigment contents improved significantly on Murashige and Skoog (MS) medium (solid and liquid) containing ...in ...
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The retention of differentiated function in cell culture : a study of the UDCK cell line
... the cell monolayers were rinsed briefly (4x) with ice-cold Krebs* solution (twenty seconds) to remove any extracellular ...of cell suspension prepared by trypsinisation as described ...above). ...
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In silico characterization of cell–cell interactions using a cellular automata model of cell culture
... of cell population ...include cell–cell adhesion effects to simulate cultured cells in 2D and 3D [19, 20, ...the cell–cell interaction properties of cultured ...the ...
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Mechanisms of platelet stimulated colon cancer invasion: role of clusterin and thrombospondin 1 in regulation of the P38MAPK MMP 9 pathway
... platelet–tumour cell interactions were ...and cell inva- sion ...platelet–cancer cell interactions was investi- gated using stable isotope labelling by amino acids in cell culture ...
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A Bioengineered Three Dimensional Cell Culture Platform Integrated with Microfluidics To Address Antimicrobial Resistance in Tuberculosis
... broth culture, where bacilli are rapidly dividing under optimal growth ...host cell biology is modulated by three-dimensional (3D) extracellular matrix interactions, regulating key processes in the ...
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Assessment of Biological Properties of Mouse Embryonic Stem Cells Characteristics Prior To Differentiation
... ES cell proliferation con- ditions were optimized for different cell culture systems, which included the optimization of cell culture surface, cell seeding density and presence ...
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Sequence Variability of Borna Disease Virus: Resistance to Superinfection May Contribute to High Genome Stability in Persistently Infected Cells
... standard cell culture con- ditions, it is possible to isolate stable genetic virus variants from these ...uninfected cell cultures yielded 1 culture out of 30 in which a virus variant ...
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Advances in crustacean cell culture
... The second investigation involved optimisation of in vitro methods using the Australian freshwater crayfish, Cherax quadricarinatus (Chapters 6 and 7). The approach included scientifically optimising the culture ...
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Advances in crustacean cell culture
... for cell line establishment because of the numerous mitotically active germ stem cells (Luedeman and Lightner, ...immortal cell cultures using neuronal and haematopoietic stem cells of the crayfish, ...
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Tissue culture adaptation of foot-and-mouth disease virus selects viruses that bind to heparin and are attenuated in cattle.
... utilize cell surface HS to gain entry to these ...(a cell line typically used to propagate this virus) occurs more slowly and appears to result from the selection of viruses that can compete more ...
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