cell culture-derived HCV
Blocking Hepatitis C Virus Infection with Recombinant Form of Envelope Protein 2 Ectodomain
... making HCV infection the leading cause of liver transplantation in developed ...The HCV envelope glycoproteins (E1 and E2) are involved in receptor binding, virus-cell fusion, and entry into the host ...
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Development of persistent HCV genotype 3a infection cell culture model in huh 7 cell
... against HCV there is need of a cell culture based system which supports HCV ...Huh-7 cell lines for viral inoculation experiments and found that Huh-7 cell line supported the ...
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Engrafted human stem cell–derived hepatocytes establish an infectious HCV murine model
... various cell lineages offers great prom- ise for in vitro study and cell therapy of various diseases (1, ...patient-specific cell types of all lineages ...vitro culture of PHHs (9). The in ...
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Extracellular Interactions between Hepatitis C Virus and Secreted Apolipoprotein E
... extracellular HCV particles in a manner dependent on the C-terminal domain of ...293T cell culture medium as well as recombinant ApoE (rApoE) protein expressed by ...concentrated HCV core ...
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Efficient Infectious Cell Culture Systems of the Hepatitis C Virus (HCV) Prototype Strains HCV-1 and H77
... were derived from Huh7 cells but are deficient in CD81 and thus support only HCV RNA rep- lication and virus particle assembly and release but not infection ...(43). HCV-1 genomes containing only ...
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Development of an Intergenotypic Hepatitis C Virus (HCV) Cell Culture Method To Assess Antiviral Susceptibilities and Resistance Development of HCV NS3 Protease Genes from HCV Genotypes 1 to 6
... and 2.5% nucleotide and amino acid sequence divergence, respectively, from each other, again representing the naturally occurring diversity of this genotype (20, 27, 57, 60) (see Fig. S1A in the supplemental material at ...
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A Novel Strategy To Develop a Robust Infectious Hepatitis C Virus Cell Culture System Directly from a Clinical Isolate
... the HCV field was greatly enhanced by constructing infectious cDNA clone of ...efficient HCV culture systems have been established by using consensus sequence-based ...infectious HCV cDNA ...
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Hepatitis C Virus Causes Uncoupling of Mitotic Checkpoint and Chromosomal Polyploidy through the Rb Pathway
... for HCV core protein expression. Primary hepatocytes were obtained from Cell Culture Core Facility at the University of Southern ...for HCV infection using the culture supernatant of an ...
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PubMedCentral-PMC5175173.pdf
... chronic HCV-induced advanced liver diseases such as ...chronic HCV–induced liver disease, and are generally derived from circulating inflammatory monocytes; however very little is known about their ...
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Hepatitis C Virus Attachment Mediated by Apolipoprotein E Binding to Cell Surface Heparan Sulfate
... purified HCV particles and that HCV infectivity was proportional to the abundance of apoE in infectious HCV ...purified HCV was found to be sensitive to protease digestion, suggesting its ...
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Adaptive Mutations Enhance Assembly and Cell-to-Cell Transmission of a High-Titer Hepatitis C Virus Genotype 5a Core-NS2 JFH1-Based Recombinant
... infectious HCV cell culture (HCVcc) system has been of great importance for HCV research since its develop- ment in 2005 ...recombinant culture sys- tems, as well as full-length ...
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Comparative Analysis of Anti-Hepatitis C Virus Activity and Gene Expression Mediated by Alpha, Beta, and Gamma Interferons
... of HCV replicon replication induced by IFN-␥ at all doses tested after 24 h was not reversible over a 6-day ...of HCV replicon replication after 24 h induced by IFN-␣ and - was reversible even when the ...
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Tissue culture and frost tolerance studies in Solanum
... 87 Callus cultures 87 Callus initiation Callus culture multiplication 93 103 Cell suspension cultures Tuber callus-derived cell suspensions 103 108 Stem callus-derived cell suspensions M[r] ...
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Phytochemical screening and antioxidant activity of leaves and cell suspension culture from seed derived callus of Spinacia Oleracae
... seed derived callus formation was maximum after 28 th day and satisfactory establishment of cell suspension culture was observed after the 25 th day of ...of cell culture extract was ...
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NS3 is a serine protease required for processing of hepatitis C virus polyprotein.
... To characterize the molecular aspects of HCV protein biogenesis and to clearly identify the protein products derived from the HCV genome, we have examined HCV polyprotein expression by u[r] ...
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Inosine Triphosphate Pyrophosphatase Dephosphorylates Ribavirin Triphosphate and Reduced Enzymatic Activity Potentiates Mutagenesis in Hepatitis C Virus
... inducing HCV mutagenesis at higher ...the HCV RNA-dependent RNA polymerase, which lacks proofreading, would cause the observed mutation pat- ...the HCV genome, incorrect incorporation of RTP into the ...
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Culture expansion of adipose derived stromal cells A closed automated Quantum Cell Expansion System compared with manual flask based culture
... manual cell culture to expansion of cells in Quantum systems demands biological coat- ing in order to allow attachment of ...for culture expansion of BM-MSCs, have used fibronectin as a coating mate- ...
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Promoting the expansion and function of human corneal endothelial cells with an orbital adipose-derived stem cell-conditioned medium
... critical cell density (<1000 cells/mm 2 ) due to a pathological status such as Fuchs endothelial corneal dystrophies, intraocular surgery, or trauma [1, ...
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Suppression of Viral RNA Binding and the Assembly of Infectious Hepatitis C Virus Particles In Vitro by Cyclophilin Inhibitors
... potent HCV inhibitor BMS-790052, which most likely tar- gets domain I of NS5A, had no significant effect on RNA ...with HCV RNA, presumably via the RNA-binding groove re- vealed by crystal ...bind ...
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DNA-based immunization with chimeric vectors for the induction of immune responses against the hepatitis C virus nucleocapsid.
... Isotyping of anti-HCV capsid sera. At 6 weeks p.i., sera from mice immunized with the chimeric vectors were tested to de- termine the major anticapsid isotype by using a mouse hybrid- oma subtyping kit (Boehringer ...
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