Cell-free system
A sea urchin cell free system to study male pronuclear assembly and activation
... a cell-free system derived from homogenates of activated sea urchin egg ...The cell-free system has provided several novel insights including requirements for sperm-specific ...
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Expression of Functional Plasmodium falciparum Enzymes Using a Wheat Germ Cell-Free System
... P. falciparum enzyme synthesis. All selected P. falciparum en- zymes were first generated in a batch reactor with radioactive leu- cine and a native malarial coding sequence (Fig. 1A). The majority of the proteins even ...
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The influence of the penetrating cryoprotectors on protein synthesis in the cell-free system of the rat liver
... tivity of postmitochondrial supernantant of the rat liver as well as on aminoacylation processes has been investigated. The addition of the penetrating cryoprotectors – ethylene glycol and DMSO – re sulted in the ...
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Pyridine nucleotide dependent superoxide production by a cell free system from human granulocytes
... prepared from human granulocytes formed O2- at a rate of 18. 4 +/- 4.6 SE nmol/ml reaction mixture per donor, but not with glucose-6-phosphate, 6-phosphogluconate, glyceraldehyde- 3-phosphate, sodium lactate, ...
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Folding of Hepatitis C Virus E1 Glycoprotein in a Cell-Free System
... mammalian cell expression systems have focused primarily on E2 and its folding, whereas knowledge of E1 folding remains ...a cell-free in vitro translation system to study E1 folding and asked ...
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Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system.
... The polypeptide molecular weight, 56,000, synthesized in a cell-free reaction, that comigrates with authentic NP has a tryptic peptide pattern identical to the tryptic peptides of NP fro[r] ...
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Translation and processing of mouse hepatitis virus virion RNA in a cell-free system.
... Either leupeptin or zinc chloride was added to the lysate before the addition of MHV-A59 mRNA, and translation reactions were incubated for 90 min in the presence of [35S]methionine: Lan[r] ...
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Phosphorylation of Simian Virus 40 Proteins in a Cell-Free System
... After phosphorylation in vitro of either empty capsids or infectious virus by beef heart kinase, the virus particles were banded in KBr and precipitated with 10% trichloroacetic acid.. S[r] ...
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Responding to chromosomal breakage during M-phase: insights from a cell-free system
... study cell cycle progression and DNA damage checkpoints. Recently this system has been successfully used to uncover an ATM and ATR dependent checkpoint affecting centrosome driven spindle ...mitotic ...
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Effects of temperature and lipophilic agents on poliovirus formation and RNA synthesis in a cell-free system.
... In view of the proposed role of membranes in poliovirus replication in vivo, and a report of an inhibitory effect of oleic acid 9-octadecenoic acid on poliovirus RNA replication 9, we ha[r] ...
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Human immunodeficiency virus integration in a cell-free system.
... To determine whether any nucleotides or other small molecules were required as cofactors for HIV integration, the crude cytoplasmic extract from infected cells was fractionated by gel ex[r] ...
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Inhibition of binding to initiation complexes of nascent reovirus mRNA by double-stranded RNA-dependent protein kinase.
... Of necessity, experiments designed to examine whether the binding of nascent reovirus mRNA to initiation complexes was preferentially inhibited in the coupled, cell-free system had to be[r] ...
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The membrane-associated and secreted forms of the respiratory syncytial virus attachment glycoprotein G are synthesized from alternative initiation codons.
... Analysis of the proteins synthesized from these cDNAs, either by translation of transcripts in a cell-free system or in cells infected with recombinant vaccinia viruses containing either[r] ...
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Stögbauer, Tobias Roland (2012): Experiment and quantitative modeling of cell-free gene expression dynamics. Dissertation, LMU München: Fakultät für Physik
... particular cell-free system which is generally not ...a cell-free system through a combination of a set of calibration experiments and a mathematical model is ...of ...
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Expression of a Sheep Pox Virus Gene in Plant Systems under the Control of Plant Viral Regulatory Elements and with Sub-Cellular Targeting
... Plasmids were linearized with EcoRI before in vitro synthesis of uncapped full-length mRNAs. After transcription, the mRNAs were isolated and checked for integrity by electrophoresis in 1 % agarose. Equal amounts of ...
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Protein synthesis in a cell free human reticulocyte system: ribosome function in thalassemia
... In the present investigation, a cell free system for protein synthesis has been developed from human reticulocytes and this latter hypothesis tested further by comparing the capacity for[r] ...
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Phosphate Acceptor Amino Acid Residues in Structural Proteins of Rhabdoviruses
... When vesicular stomatitis or rabies virus proteins were phosphorylated in a cell-free system by the virion-associated protein kinase and analyzed for the presence of phosphorylated amino[r] ...
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Translation of murine leukemia virus RNA in cell-free systems from animal cells.
... Fingerprint analysis of tryptic digests of polypeptide products formed in the cell-free system in B Polypeptides 60 and 70K synthesized in the L-cell-free system to 140,000-molecular-wei[r] ...
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Prototyping Diverse Synthetic Biological Circuits in a Cell Free Transcription Translation System
... solubilized cell membrane using detergents, liposomes, or other membrane-like ...transformation, cell growth, lysis, membrane solubilization, and purification are involved ...in cell free ...
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Cell free expression and in meso crystallisation of an integral membrane kinase for structure determination
... open system the protein can be harvested directly without the need for all that goes with culturing live cells and isolating membranes or inclusion bodies from harvested ...The cell-free synthesised ...
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