Cell lysates
Rapid detection by a coagglutination test of heat labile enterotoxin in cell lysates from blood agar grown Escherichia coli
... Cell lysates from human and animal ETEC strains grown on blood agar were compared for LT production by the coa-test, with staphylococci coated with anti-LT antibodies, and by the CHO cel[r] ...
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Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
... The aim here was to apply the developed method for RNA expression monitoring of fermentation processes. Recent- ly, this was performed by Jürgen et al. [13] and Schweder et al. [17] by slot blot analysis, which is in ...
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RNase activity in erythroid cell lysates
... nucleotides in cell lysates. Competitive inhibition studies indicate that one enzyme system is capable of degrading both RNA and ribosomes, although the existence of more than one enzyme has not been ...
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Development of two novel high throughput assays to quantify ubiquitylated proteins in cell lysates: application to screening of new anti malarials
... Percentage of stimulation of iRBCs treated with the proteasome inhibitor MG132 or lactacystin (control 2) as well as of iRBCs without treatment (control 1), is rep- resented in Figure 6B. These results were obtained from ...
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Methods for the measurement of a bacterial enzyme activity in cell lysates and extracts
... freeze-thawing cell suspensions and separating the cell envelope and cytosolic fractions by high-speed ...of cell lysis has several advantages over other methods in the study of enzyme ...of ...
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Specific proteolytic fragmentation of p60v-src in transformed cell lysates.
... The enzyme preparation containing the proteolytic breakdown product of p60v-src more vigorously phosphorylated the antisrc IgG, whereas both casein and tubulin appeared to be phosphoryla[r] ...
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GMP 140, a platelet alpha granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel Palade bodies
... We used an immunoperoxidase procedure to examine the tissue distribution of the platelet alpha-granule membrane protein, GMP-140. In addition to its presence in megakaryocytes and platelets, GMP-140 antigen was found in ...
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Envelope glycoprotein and CD4 independence of vpu-facilitated human immunodeficiency virus type 1 capsid export.
... The amounts of the envelope glycoproteins gp160 and gp120 present in HeLa-T4 cell lysates transfected with HXBH10-vpu+/env+ and HXBH10-vpu-/env+ DNAs are similar in lysates of cells labe[r] ...
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Proteins associated with human parainfluenza virus type 3.
... The viral origin of the polypeptides was further confirmed by immunoprecipitation of infected cell lysates with rabbit hyperimmune antisera against the envelope and core fractions of pur[r] ...
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Self Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus Like Particles and Evaluation of Cross Reactivity of BoNV with Human Noroviruses
... from cell culture super- natants and cell lysates of Sf9 cells infected with rCV186-OH on CsCl density gradients and sucrose cushions and reacted with the hyperimmune antisera Bo9114 ...OH-infected ...
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Cross presentation of viral antigens in dribbles leads to efficient activation of virus specific human memory t cells
... or cell lysates derived from HEK293T or UbiLT3 cell lines expressing cytomegalovirus (CMV) pp65 protein or transfected with a plasmid encoding dominant HLA-A2 restricted CMV, Epstein-Barr virus ...
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Multicenter phase II study of matured dendritic cells pulsed with melanoma cell line lysates in patients with advanced melanoma
... tumor cell lysates administered with BCG and the other used a ganglioside vaccine administered with a strong KLH adjuvant ...T cell infiltrates in tumors [14], attesting to the immune nature of their ...
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REAP: A two minute cell fractionation method
... tic cell scraper and collected in ...and cell pellets were resuspended in 900 μ L of ice-cold ...“whole cell lysate” and 100 μL of 4 × Laemmli sample buffer was added to it, then kept on ice until ...
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Epizootic Hemorrhagic Disease Virus Induces and Benefits from Cell Stress, Autophagy, and Apoptosis
... extensive cell rounding ...OK cell cultures with UV-irradiated virus, separated cell lysates by SDS-PAGE, and probed for NS3, LC3, and ...
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Application of antibody to synthetic peptides for characterization of the intact and truncated alpha 22 protein specified by herpes simplex virus 1 and the R325 alpha 22- deletion mutant.
... Lane 1, Autoradiographic images of HSV-1F-infected F cell lysates used as markers; lane 2, autoradiographic images of mock-infected M HEp-2 cell lysates; lane 3, immunoreactivity of lane[r] ...
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Arenavirus Z-Glycoprotein Association Requires Z Myristoylation but Not Functional RING or Late Domains
... The cell lysates shown in panel B were subjected to Flag immunoprecipitation and Western blotting to detect Z WT strains and Z ...whole-cell lysates, the amount of Z mutants present in Flag ...
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Cell-Free Replication of the Hepatitis C Virus Subgenomic Replicon
... the lysates (see ...of lysates from transiently transfected Huh-7 cells with HCV subgenomic replicon RNA (BM4-5) and FCA4 cell lysates treated with micrococcal ...with lysates prepared ...
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The herpes simplex virus 1 protein kinase encoded by the US3 gene mediates posttranslational modification of the phosphoprotein encoded by the UL34 gene.
... 3B show that i in the infected cell lysates of R7306, the phosphoprotein profile was that of the wild-type HSV-1F and ii the phosphoprotein profile of the lysates of cells infected with [r] ...
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Reduction of circulating soluble Flt-1 alleviates preeclampsia-like symptoms in a mouse model
... in cell lysates, liver lysates or kidney ...from cell lysates and liver lysates after aden- ovirus treatment 1–6 mg protein was incubated over 2 hr (cell lysate) or 16 hr ...
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Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability
... crude cell lysates were mixed with the Ni-NTA resin and incubated at 4 °C for 2 ...surplus cell lysates flowed through the column while target protein was ...
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