CFP-10-ESAT-6 complex
Assessment of Cross-Reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-Cell Epitope Level
... (MAI) complex (for example, 54% of the NTM isolates in Great Britain from 2004 to ...for ESAT-6 homologues (4) and most likely also genes for CFP-10 homo- ...MAI complex (46%) ...
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Selected Pool of Peptides from ESAT 6 and CFP 10 Proteins for Detection of Mycobacterium tuberculosis Infection
... isolates with the exception of M. kansasii, M. marinum, M. szulgai, M. flavescens, and M. gastrii (6, 19, 31, 38). An ESAT-6 homologue has also been identified in the genome of M. leprae (18). Both ...
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A T cell based assay using specific Mycobacterium tuberculosis antigens, ESAT-6 and CFP 10, for the diagnosis of Tuberculous Meningitis.
... The development of cell-mediated immunity against M tuberculosis is associated with the development of a positive result in the tuberculin skin test. At the cellular level, an effective host immune response occurs as ...
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Use of Synthetic Peptides Derived from the Antigens ESAT-6 and CFP-10 for Differential Diagnosis of Bovine Tuberculosis in Cattle
... from ESAT-6, MPB70, MPB83, and MPB64 differentiates between infected and BCG-vacci- nated ...antigens ESAT-6 and CFP-10. ESAT-6 is a well-studied antigen found in ...
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Assessment of a Protein Cocktail-Based Skin Test for Bovine Tuberculosis in a Double-Blind Field Test in Cattle
... of CFP-10 and ESAT-6 (6, ...and ESAT-6 in order to strengthen the sensitivity of the skin ...tuberculosis complex and Mycobacterium avium com- plex (MAC), and can ...
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Construction of Eukaryotic Expression Vectors Encoding CFP-10 and ESAT-6 Genes and Their Potential in Lymphocyte Proliferation
... The 10-kDa culture filtrate protein (CFP-10) and 6-kDa early-secreted target antigen (ESAT-6) are two low molecular weight secretory proteins encoded by the Rv3874 and Rv3875 ...
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Rapid Identification of the Mycobacterium tuberculosis Complex by Combining the ESAT 6/CFP 10 Immunochromatographic Assay and Smear Morphology
... the ESAT-6/CFP-10 ICA, all of the 10 false- negative and 7 false-positive cultures were assayed again 1 week after the initial ...secrete ESAT-6 and CFP-10, ...
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Immune Responses to Defined Antigens of Mycobacterium bovis in Cattle Experimentally Infected with Mycobacterium kansasii
... Molecular and traditional microbiological methods are readily available to distinguish between M. bovis and M. kan- sasii infection upon collection of tissues at necropsy. The iso- lation of nontuberculous mycobacteria ...
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Development of a Skin Test for Bovine Tuberculosis for Differentiating Infected from Vaccinated Animals
... as ESAT-6 and CFP-10 for DIVA has so far been realized largely through their application in the blood-based gamma interferon release ...tuberculosis complex recombinant protein antigens ...
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Serodiagnosis Efficacy and Immunogenicity of the Fusion Protein of Mycobacterium tuberculosis Composed of the 10-Kilodalton Culture Filtrate Protein, ESAT-6, and the Extracellular Domain Fragment of PPE68
... the 6-kDa early secretory antigenic target (ESAT-6) (2), Ag85B (17), MPT64 (12), PPE68 (22), and culture-filtered protein 10 (CFP- 10) (5), isolated from short-term culture ...
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Prospective Evaluation of a Whole-Blood Test Using Mycobacterium tuberculosis-Specific Antigens ESAT-6 and CFP-10 for Diagnosis of Active Tuberculosis
... antigens ESAT-6 and CFP-10 (4, 5, 26) has led to the development of new specific diagnostic tests for infection with ...(2). ESAT-6 and CFP-10 are contained within ...
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Lymphocyte proliferation to mycobacterial antigens is detectable across a spectrum of HIV associated tuberculosis
... vs. 26.6%, P < 0.0001; Ag85, 38.6% vs. 24.8%, P < 0.0001; WCL, 78.4% vs. 42.8%, P < 0.0001), but IFN-γ responses were not consistently different between subjects with and without active TB (ESAT-6, ...
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Genomics and the evolution, pathogenesis, and diagnosis of tuberculosis
... tuberculosis complex The Mycobacterium tuberculosis complex presently consists of seven species and subspecies that include ...tuberculosis complex behaves as an ecotype, with preferred mam- malian ...
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Response to M tuberculosisselected RD1 peptides in Ugandan HIV infected patients with smear positive pulmonary tuberculosis: a pilot study
... A recent breakthrough in the diagnosis of M. tuberculosis infection has been the development of T-cell-based inter- feron(IFN)-gamma release assays (IGRAs) that use anti- gens belonging to M. tuberculosis region of ...
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Evaluation of Gamma Interferon Release Assays Using Mycobacterium tuberculosis Antigens for Diagnosis of Latent and Active Tuberculosis in Mycobacterium bovis BCG-Vaccinated Populations
... to ESAT-6 and ...to ESAT-6 detected by the ELISPOT (P ⫽ ...to ESAT-6 and CFP-10 detected by the ELISPOT (both P ⬍ ...
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IL 17 and IL 22 production in HIV+ individuals with latent and active tuberculosis
... Fig. 6 Mean values of IL-23R, ICOS and FoxP3 expression on CD4 + T cells after PD-1 neutralization. Freshly isolated PBMC (8 donors in each group) from HIV + LTBI+ individuals, and HIV + TB patients were cultured ...
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Specificity of the Tuberculin Skin Test Is Modified by Use of a Protein Cocktail Containing ESAT-6 and CFP-10 in Cattle Naturally Infected with Mycobacterium bovis
... protein cocktail; however, the best results were obtained using 20 g of the cocktail with 72 h as the time selected for reading. Our results are consistent with those reported by Whelan et al. (29); they identified ...
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Kinetics of Mycobacterium tuberculosis-specific IFN-gamma responses and sputum bacillary clearance in HIV-infected adults during treatment of pulmonary tuberculosis
... PPD, ESAT-6 and ...0.05; CFP-10 19 vs. 74 SFU, p ¼ 0.01). ESAT-6-speci fi c IFN- g secreting cells and sputum bacillary load declined progressively during treatment in both ...
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Kinetics of Mycobacterium tuberculosis specific IFN γ responses and sputum bacillary clearance in HIV infected adults during treatment of pulmonary tuberculosis
... 0.25 10 6 PBMCs at 10 m g/ml puri fi ed protein derivative (PPD) (Statens Serum Institute, Copenhagen, Denmark), 5 m g/ml CFP-10, 5 m g/ml ESAT-6 (Peptide and Protein ...
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The Secreted Protein Rv1860 of Mycobacterium tuberculosis Stimulates Human Polyfunctional CD8+ T Cells
... We previously reported that Rv1860 protein from Mycobacterium tuberculosis stimulated CD4 ⴙ and CD8 ⴙ T cells secreting gamma interferon (IFN-␥) in healthy purified protein derivative (PPD)-positive individuals and ...
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