CFU-F
Modulation of murine bone marrow-derived CFU-F and CFU-OB by in vivo bisphosphonate and fluoride treatment
... μg/kg/week; F 0 ppm; Group 2 = ALN 3 μg/kg/week; F 50 ppm; Group 3 = ALN 30 μg/kg/week; F 0 ppm; Group 4 = ALN 30μg/kg/week; F 50 ppm; Group 5 = ALN 150 μg/kg/week; F 0 ppm; Group 6 = ...
81
Hierarchy of mesenchymal stem cells: Comparison of multipotentmesenchymal stromal cells with fibroblast colony forming units
... and CFU-F. MMSC were presented by more immature cells than CFU-F and took up the higher position in hierarchical tree of mesenchy- mal stem ...and CFU-F ...
8
Does osteogenic potential of clonal human bone marrow mesenchymal stem/stromal cells correlate with their vascular supportive ability?
... CXCR4 on endothelial cells. This serves to enhance vas- cular network formation by allowing endothelial tip-like cells to extend towards the CXCL12 guidance cue on ad- jacent endothelial cells or by promoting the ...
15
The effect of acute respiratory distress syndrome on bone marrow-derived mesenchymal stem cells
... the metabolic activity of MSCs from all groups with a con- comitant increase in cell proliferation on day 7 (Fig. 3c). The same inverse relationship was observed when MSCs were cocultured with MNCs (Fig. 6a, b). It is ...
13
Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media
... In this study, 19 leftover lipoaspirates were processed for SVF extraction, with 14 lipoaspirates being taken from the abdomen and the remaining samples from the thigh or hip/flank. The first six lipoaspirates were imme- ...
16
Osteogenic differentiation of dental pulp stem cells under the influence of three different materials
... differentiation; CFU-F: Colony forming unit-fibroblast; DMEM: Dulbecco ’ s modified eagles medium (with high glucose, sodium pyrovate and L-glutamine); DNA: Deoxy-ribionucleic acid; DPSCs: Dental pulp stem ...
10
Tracing the destiny of mesenchymal stem cells from embryo to adult bone marrow and white adipose tissue via Pdgfrα expression
... (CFU- F) assays on the sorted cells (Fig. 1A, Fig. S1A). Colonies of CFU-F initially emerged at embryonic day ...increased CFU-F colony numbers during embryo development, the ...
9
Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
... albumin; CFU-F: colony formation units of fibroblastic cells; CNPase: 2 ’ , 3 ’ -cyclic nucleotide-3 ’ - phosphodiesterase; (D)MEM: (Dulbecco ’ s) modified Eagle ’ s medium; DPSCs: dental pulp stem cells; ...
11
Mesenchymal stromal cells: Biology of adult mesenchymal stem cells: regulation of niche, self renewal and differentiation
... αSMA = α-smooth muscle actin; bHLH = basic helix–loop–helix; BMP = bone morphogenetic protein; CFU-F = colony-forming unit-fibroblast; ECM = extracellular matrix; FGF = fibroblast growth[r] ...
10
Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: present and future
... blood; CFU-F, fibroblastoid colony-forming unit; EGF, epidermal growth factor; FBS, fetal bovine serum; GMP, Good Manufacturing Practices; GVHD, graft versus host disease; HGF, hepatocyte growth factor; ...
8
Exosomes of human placenta-derived mesenchymal stem cells stimulate angiogenesis
... DNA; CFU-F: Fibroblast colony-forming units; CM: Conditioned medium; DLS: Dynamic light scattering; D-MEM: Dulbecco ’ s modified Eagle ’ s medium; eNOS: Endothelial nitric oxide synthase; FBS: Fetal bovine ...
12
Efficacy of autologous stem cell-based therapy for osteonecrosis of the femoral head in sickle cell disease: a five-year follow-up study
... Our study did not allow study of the relationship be- tween the clinical outcome and the number or phenotypic profile of cells implanted per volume of lesion. We found substantial donor-dependent variations and high ...
18
Regulation of haematopoietic stem cycle (CFU S) proliferation in irradiated mice
... o f CFÜ-S however was more obvious when repeated ir r a d ia tio n was performed allow ing time; for repopulation between doses (Hendry and Lajtha, ...The CFU-S population in the femur and sp leen recovered ...
230
Effects of salubrinal on development of osteoclasts and osteoblasts from bone marrow derived cells
... the osteoporotic RANKL-injected mice than those isolated from the control mice? Because of the anticipated role of salubrinal that is potentially opposite to that of RANKL, we hypothesized that salubrinal is more ...
11
Quantitation of Candida CFU in Initial Positive Blood Cultures
... minimum CFU/ml in an adult is 0.1 CFU/ml (1 colony/10 ml); for an infant that value is ...1 CFU/ml (instead of 5 CFU/ml) demonstrated the highest association of pediatric age with organism ...
5
Tumor-derived extracellular vesicles inhibit osteogenesis and exacerbate myeloma bone disease
... BV/TV and Tb.Th were significantly lower in the MM+MM-EVs group than in the MM group. (Figures 4B-F). Representative 3D figures and section maps of the separated trabecular bones are shown in Figure 4G-I. In ...
14
DNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice
... Genes virB9 and virB12 from B. abortus, ligated into pET-47b plasmid (Novagen ® ), were introduced into competent E. coli TOP10 F’ for storage and culture. Stored strains were cultured in liquid LB broth and ...
14
Contribution of different pneumococcal virulence factors to experimental meningitis in mice
... Mice infected with the PspA-deficient strain FP262 showed increased survival and prolonged time-to-death for all bacterial inocula as well as lower viable counts in both blood (at the doses of 10 2 and 10 3 ...
11
Interaction of murine granulocyte macrophage progenitors and supporting stroma involves a recognition mechanism with galactosyl and mannosyl specificities
... (representing CFU- C bound to stroma) ...of CFU-C to supporting stroma. To determine if CFU-C possesses homing receptors with these specificities, we induced agglutination in marrow cell suspensions ...
9
Association between colony forming units granulocyte macrophage expression of Ia like (HLA DR) antigen and control of granulocyte and macrophage production A new role for prostaglandin E
... by CFU-GM is lost within 3-6 h of culture at 37 degrees C and occurs simultaneously with loss of responsiveness to inhibition by these ...marrow CFU-GM in a limited exposure suspension culture with 1 ...
12