Chloramphenicol acetyltransferase
Chloramphenicol acetyltransferase as a selection marker for chlamydial transformation
... these methods were established with plasmids carrying a β- lactamase, which degrades penicillin family antibiotics, and thus relieves their inhibitory effects of these drugs on the cytokinesis of RBs [8,9]. Since ...
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Rescue of a synthetic chloramphenicol acetyltransferase RNA into influenza virus-like particles obtained from recombinant plasmids.
... virus-like chloramphenicol acetyltransferase (CAT) RNA is achieved in cells infected with recombinant vaccinia virus vTF7-3, which expresses the T7 RNA polymerase, and trans- fected with four pGEM-derived ...
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Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables isobutyl acetate production directly from cellulose by Clostridium thermocellum at elevated temperatures
... Chloramphenicol acetyltransferase (E.C. 2.3.1.28, CAT) is another acetyltransferase class that has been found in various microbes ...confers chloramphenicol resistance in ...
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Virus-induced modification of the host cell is required for expression of the bacterial chloramphenicol acetyltransferase gene controlled by a late herpes simplex virus promoter (VP5).
... 19 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The requirements for expression of genes under the control of early alkaline exonuclease and late VP5 herpes simplex [r] ...
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A poliovirus replicon containing the chloramphenicol acetyltransferase gene can be used to study the replication and encapsidation of poliovirus RNA.
... B A CAT-specific protein with an Mr of 51,000 CAT-VP3 fusion indicated by the arrow is present in extracts of cells transfected with FLC/REP RNA lane 3 but is absent from mock-transfecte[r] ...
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Stimulation of chloramphenicol acetyltransferase mRNA translation by reovirus capsid polypeptide sigma 3 in cotransfected COS cells.
... Stimulation of CAT mRNA translation by the S4 gene product in cotransfected COS cells is at first sight paradoxical in light of the known segregation of the S4 gene with the serotype-dep[r] ...
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Induction of CD4 expression and human immunodeficiency virus type 1 replication by mutants of the interferon-inducible protein kinase PKR.
... To further substantiate the activation of NF-kB by PKRLS9, the chloramphenicol acetyltransferase CAT reporter gene under the control of the wt HIV-1 LTR ptzIIICAT [6] or a mutant form of[r] ...
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Arsenic trioxide inhibits nuclear receptor function via SEK1/JNK mediated RXRα phosphorylation
... CAT, chloramphenicol acetyltransferase; ER, estrogen receptor; LXR, liver X receptor; MEF, mouse embryo fibroblast; p-, phosphorylated; PML, promyelo- cytic leukemia; RA, all-trans retinoic acid; RAR, RA ...
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Inhibition of calcitriol receptor binding to vitamin D response elements by uremic toxins
... Abbreviations used in this paper: CAT, chloramphenicol acetyltransferase; EMSA, electrophoretic mobility shift assay; GH, growth hormone; Nm..,, maximal binding capacity; N-UF, normal ul[r] ...
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Identification of the hepatitis B virus factor that inhibits expression of the beta interferon gene.
... The chloramphenicol acetyltransferase CAT-containing plasmids that were used in this study were i pTWU54, in which the cat gene is under the control of the 353-bp HindIll-HincIl DNA regi[r] ...
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The CAGT motif functions as an initiator element during early transcription of the baculovirus transregulator ie-1.
... To determine the function of the CAGT motif during ie-1 expression, we analyzed mutated ie-1 promoters that were fused to the chloramphenicol acetyltransferase CAT reporter gene by using[r] ...
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Localization of sequences responsible for trans-activation of the equine infectious anemia virus long terminal repeat.
... 120 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We used the Escherichia coli chloramphenicol acetyltransferase gene cat to study sequences that influence expression[r] ...
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Activation of human papillomavirus type 18 gene expression by herpes simplex virus type 1 viral transactivators and a phorbol ester.
... A plasmid containing the HPV-18 noncoding region placed upstream of the chloramphenicol acetyltransferase reporter gene was cotransfected with different herpes simplex virus type 1 HSV-1[r] ...
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Negative regulatory element associated with potentially functional promoter and enhancer elements in the long terminal repeats of endogenous murine leukemia virus-related proviral sequences.
... 2746 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Three series of recombinant DNA clones were constructed, with the bacterial chloramphenicol acetyltransferase CAT g[r] ...
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The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure.
... Using constructs which direct synthesis of RNA containing a chloramphenicol acetyltransferase CAT gene between mutagenized influenza virus noncoding sequences derived from the NS genome [r] ...
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Identification of upstream promoter elements mediating early transcription from the 35,000-molecular-weight protein gene of Autographa californica nuclear polyhedrosis virus.
... The promoter fragment, extending from positions -226 to +12 relative to the early RNA start site position +1, was fused to the reporter gene encoding chloramphenicol acetyltransferase CA[r] ...
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Influenza B viruses with site-specific mutations introduced into the HA gene.
... the chloramphenicol acetyltransferase gene flanked by the noncoding sequences of the HA or NS genes of influenza B virus were transfected into cells which were previously infected with an influenza B helper ...
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Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization.
... GB viruses A and C (GBV-A and GBV-C) are two recently described RNA viruses which appear to be members of the Flaviviridae. Although both viruses appear to contain long 5 * nontranslated regions, the sites of polyprotein ...
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A single regulatory region modulates both cis activation and trans activation of the herpes simplex virus VP5 promoter in transient-expression assays in vivo.
... 460 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest A detailed analysis of the expression of the bacterial chloramphenicol acetyltransferase gene controlled by the herp[r] ...
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Tumor necrosis factor alpha and interferon gamma suppress the activation of human type I collagen gene expression by transforming growth factor beta 1 Evidence for two distinct mechanisms of inhibition at the transcriptional and posttranscriptional levels
... (chloramphenicol acetyltransferase) construct, while posttranscriptional regulation was assessed by parallel determinations of type I procollagen mRNA steady-state ...
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